TY - JOUR
T1 - Click chemistry mediated functionalization of vertical nanowires for biological applications
AU - Vutti, Surendra
AU - Schoffelen, Sanne
AU - Bolinsson, Jessica
AU - Buch-Månson, Nina
AU - Bovet, Nicolas Emile
AU - Nygård, Jesper
AU - Martinez, Karen Laurence
AU - Meldal, Morten Peter
N1 - [QDev]
PY - 2016
Y1 - 2016
N2 - Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the CuI-catalyzed alkyne-azide cycloaddition and its strain-promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.
AB - Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the CuI-catalyzed alkyne-azide cycloaddition and its strain-promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.
KW - alkyne-azide cycloaddition
KW - biosensors
KW - diazotransfer
KW - nanowires
KW - protein immobilization
U2 - 10.1002/chem.201504540
DO - 10.1002/chem.201504540
M3 - Letter
C2 - 26601641
AN - SCOPUS:84953282873
SN - 0947-6539
VL - 22
SP - 496
EP - 500
JO - Chemistry: A European Journal
JF - Chemistry: A European Journal
IS - 2
ER -