Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing

Stefano Stella; Pablo Alcón; Guillermo Montoya

doi:10.1038/nsmb.3486

Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing

Stefano Stella, Pablo Alcón, Guillermo Montoya

Protein Structure and Function Program

30 Citations (Scopus)

Abstract

CRISPR-Cas is a bacterial defense system against phage infection and nucleic acid invasion. Class 2 type II CRISPR-Cas9 has also been widely used for genome engineering. Here, we review novel insights into the CRISPR class 2 type V enzymes, specifically Cpf1 and C2c1, which display different DNA-recognition and cleavage characteristics than those of Cas9, the best-characterized member of class 2. Recent structures of these ribonucleoprotein complexes that capture several stages of the endonuclease reaction have provided molecular details of recognition, unzipping and cleavage of the target DNA, allowing their comparison with Cas9. A detailed understanding of these mechanisms is crucial for improving these genome engineering tools and expanding the genomic space that can be targeted.

Original language	English
Journal	Nature Structural and Molecular Biology
Volume	24
Issue number	11
Pages (from-to)	882-892
Number of pages	11
ISSN	1545-9993
DOIs	https://doi.org/10.1038/nsmb.3486
Publication status	Published - 1 Nov 2017

Keywords

CRISPR-Cas Systems
Endonucleases
Gene Editing
RNA
Recombinant Proteins

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https://www.nature.com/articles/nsmb.3486.pdf

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abstract = "CRISPR-Cas is a bacterial defense system against phage infection and nucleic acid invasion. Class 2 type II CRISPR-Cas9 has also been widely used for genome engineering. Here, we review novel insights into the CRISPR class 2 type V enzymes, specifically Cpf1 and C2c1, which display different DNA-recognition and cleavage characteristics than those of Cas9, the best-characterized member of class 2. Recent structures of these ribonucleoprotein complexes that capture several stages of the endonuclease reaction have provided molecular details of recognition, unzipping and cleavage of the target DNA, allowing their comparison with Cas9. A detailed understanding of these mechanisms is crucial for improving these genome engineering tools and expanding the genomic space that can be targeted.",

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T1 - Class 2 CRISPR-Cas RNA-guided endonucleases

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AU - Stella, Stefano

AU - Alcón, Pablo

AU - Montoya, Guillermo

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N2 - CRISPR-Cas is a bacterial defense system against phage infection and nucleic acid invasion. Class 2 type II CRISPR-Cas9 has also been widely used for genome engineering. Here, we review novel insights into the CRISPR class 2 type V enzymes, specifically Cpf1 and C2c1, which display different DNA-recognition and cleavage characteristics than those of Cas9, the best-characterized member of class 2. Recent structures of these ribonucleoprotein complexes that capture several stages of the endonuclease reaction have provided molecular details of recognition, unzipping and cleavage of the target DNA, allowing their comparison with Cas9. A detailed understanding of these mechanisms is crucial for improving these genome engineering tools and expanding the genomic space that can be targeted.

AB - CRISPR-Cas is a bacterial defense system against phage infection and nucleic acid invasion. Class 2 type II CRISPR-Cas9 has also been widely used for genome engineering. Here, we review novel insights into the CRISPR class 2 type V enzymes, specifically Cpf1 and C2c1, which display different DNA-recognition and cleavage characteristics than those of Cas9, the best-characterized member of class 2. Recent structures of these ribonucleoprotein complexes that capture several stages of the endonuclease reaction have provided molecular details of recognition, unzipping and cleavage of the target DNA, allowing their comparison with Cas9. A detailed understanding of these mechanisms is crucial for improving these genome engineering tools and expanding the genomic space that can be targeted.

KW - CRISPR-Cas Systems

KW - Endonucleases

KW - Gene Editing

KW - RNA

KW - Recombinant Proteins

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JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

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ER -

Class 2 CRISPR-Cas RNA-guided endonucleases: Swiss Army knives of genome editing

Abstract

Keywords

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