Chronic Infection with Pseudomonas aeruginosa in an animal model of oxidative stress: lessons for patients with cystic fibrosis

TY - CHAP

T1 - Chronic Infection with Pseudomonas aeruginosa in an animal model of oxidative stress: lessons for patients with cystic fibrosis

AU - Ciofu, Oana

PY - 2015

Y1 - 2015

N2 - In guinea pigs, the level of oxidative stress can be controlled by diet because, like humans, this species is not able to synthesize vitamin C (ASC), a water-soluble antioxidant. We investigated the role of oxidative stress in the outcome of infection in a guinea pig model of chronic Pseudomonas aeruginosa lung infection. Increased inflammation dominated by polymorphonuclear leukocytes (PMNs) that extensively consumed the ASC antioxidant, an increased oxidative burst of PMNs, and a poorer outcome from the infection was observed in animals experiencing diet-induced oxidative stress compared with animals with normal levels of antioxidants. These data suggest that interrupting the self-perpetuating vicious circle of infection and inflammation in patients with cystic fibrosis requires a decrease of oxidative stress. Interventions limiting reactive oxygen species production and antioxidant consumption at the site of lung inflammation, such as the prevention or treatment of P. aeruginosa infection, are highly relevant. It has been difficult to obtain evidence in randomized clinical trials in cystic fibrosis patients for the theoretical benefit of supply with antioxidants.

AB - In guinea pigs, the level of oxidative stress can be controlled by diet because, like humans, this species is not able to synthesize vitamin C (ASC), a water-soluble antioxidant. We investigated the role of oxidative stress in the outcome of infection in a guinea pig model of chronic Pseudomonas aeruginosa lung infection. Increased inflammation dominated by polymorphonuclear leukocytes (PMNs) that extensively consumed the ASC antioxidant, an increased oxidative burst of PMNs, and a poorer outcome from the infection was observed in animals experiencing diet-induced oxidative stress compared with animals with normal levels of antioxidants. These data suggest that interrupting the self-perpetuating vicious circle of infection and inflammation in patients with cystic fibrosis requires a decrease of oxidative stress. Interventions limiting reactive oxygen species production and antioxidant consumption at the site of lung inflammation, such as the prevention or treatment of P. aeruginosa infection, are highly relevant. It has been difficult to obtain evidence in randomized clinical trials in cystic fibrosis patients for the theoretical benefit of supply with antioxidants.

M3 - Book chapter

SN - 978-0-12-800051-9

SP - 171

EP - 176

BT - Diet and Exercise in Cystic Fibrosis

A2 - Ross Watson, Ronald

PB - Academic Press

CY - London

ER -