Chromogranin A in the mammalian heart: Expression without secretion

Lasse H. Hansen; Stine Darkner; Jesper H. Svendsen; Kristoffer Henningsen; Steen Pehrson; Xu Chen; Sergey Y. Vakhrushev; Katrine T. Schjoldager; Jens P. Goetze

doi:10.2217/bmm-2017-0052

Chromogranin A in the mammalian heart: Expression without secretion

Lasse H. Hansen, Stine Darkner, Jesper H. Svendsen, Kristoffer Henningsen, Steen Pehrson, Xu Chen, Sergey Y. Vakhrushev, Katrine T. Schjoldager, Jens P. Goetze^*

^*Corresponding author for this work

3 Citations (Scopus)

Abstract

Aim: To investigate whether chromogranin A (CgA) is secreted from the heart into circulation.

Materials & methods: Porcine cardiac tissue was analyzed for the presence of CgA-derived glycopeptides using a global O-glycoproteomic strategy. Blood was sampled from the femoral vein, right atrium, coronary sinus and the left atrium from patients with predominantly atrial disease. The local concentration of proatrial natriuretic peptide and CgA was measured with immunoassays.

Results: We identified CgA-derived glycopeptides exclusively in the atrial tissue. Proatrial natriuretic peptide is secreted from the heart (coronary sinus [795 pmol/l] vs left atrium [678 pmol/l]; p < 0.01) whereas no CgA gradient across the heart could be established (p = 0.6366).

Conclusion: The cardiac atria express but do not secrete CgA into circulation in patients with atrial disease.

Original language	English
Journal	Biomarkers in Medicine
Volume	11
Issue number	7
Pages (from-to)	541-545
Number of pages	5
ISSN	1752-0363
DOIs	https://doi.org/10.2217/bmm-2017-0052
Publication status	Published - Jul 2017

Keywords

atrial fibrillation
atrial natriuretic peptide
chromogranin A
heart
secretion

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@article{12215df1d7de4b24bbf5576d86ed136b,

title = "Chromogranin A in the mammalian heart: Expression without secretion",

abstract = "Aim: To investigate whether chromogranin A (CgA) is secreted from the heart into circulation. Materials & methods: Porcine cardiac tissue was analyzed for the presence of CgA-derived glycopeptides using a global O-glycoproteomic strategy. Blood was sampled from the femoral vein, right atrium, coronary sinus and the left atrium from patients with predominantly atrial disease. The local concentration of proatrial natriuretic peptide and CgA was measured with immunoassays. Results: We identified CgA-derived glycopeptides exclusively in the atrial tissue. Proatrial natriuretic peptide is secreted from the heart (coronary sinus [795 pmol/l] vs left atrium [678 pmol/l]; p < 0.01) whereas no CgA gradient across the heart could be established (p = 0.6366). Conclusion: The cardiac atria express but do not secrete CgA into circulation in patients with atrial disease.",

keywords = "atrial fibrillation, atrial natriuretic peptide, chromogranin A, heart, secretion",

author = "Hansen, {Lasse H.} and Stine Darkner and Svendsen, {Jesper H.} and Kristoffer Henningsen and Steen Pehrson and Xu Chen and Vakhrushev, {Sergey Y.} and Schjoldager, {Katrine T.} and Goetze, {Jens P.}",

year = "2017",

month = jul,

doi = "10.2217/bmm-2017-0052",

language = "English",

volume = "11",

pages = "541--545",

journal = "Biomarkers in Medicine",

issn = "1752-0363",

publisher = "Future Medicine Ltd.",

number = "7",

}

TY - JOUR

T1 - Chromogranin A in the mammalian heart

T2 - Expression without secretion

AU - Hansen, Lasse H.

AU - Darkner, Stine

AU - Svendsen, Jesper H.

AU - Henningsen, Kristoffer

AU - Pehrson, Steen

AU - Chen, Xu

AU - Vakhrushev, Sergey Y.

AU - Schjoldager, Katrine T.

AU - Goetze, Jens P.

PY - 2017/7

Y1 - 2017/7

N2 - Aim: To investigate whether chromogranin A (CgA) is secreted from the heart into circulation. Materials & methods: Porcine cardiac tissue was analyzed for the presence of CgA-derived glycopeptides using a global O-glycoproteomic strategy. Blood was sampled from the femoral vein, right atrium, coronary sinus and the left atrium from patients with predominantly atrial disease. The local concentration of proatrial natriuretic peptide and CgA was measured with immunoassays. Results: We identified CgA-derived glycopeptides exclusively in the atrial tissue. Proatrial natriuretic peptide is secreted from the heart (coronary sinus [795 pmol/l] vs left atrium [678 pmol/l]; p < 0.01) whereas no CgA gradient across the heart could be established (p = 0.6366). Conclusion: The cardiac atria express but do not secrete CgA into circulation in patients with atrial disease.

AB - Aim: To investigate whether chromogranin A (CgA) is secreted from the heart into circulation. Materials & methods: Porcine cardiac tissue was analyzed for the presence of CgA-derived glycopeptides using a global O-glycoproteomic strategy. Blood was sampled from the femoral vein, right atrium, coronary sinus and the left atrium from patients with predominantly atrial disease. The local concentration of proatrial natriuretic peptide and CgA was measured with immunoassays. Results: We identified CgA-derived glycopeptides exclusively in the atrial tissue. Proatrial natriuretic peptide is secreted from the heart (coronary sinus [795 pmol/l] vs left atrium [678 pmol/l]; p < 0.01) whereas no CgA gradient across the heart could be established (p = 0.6366). Conclusion: The cardiac atria express but do not secrete CgA into circulation in patients with atrial disease.

KW - atrial fibrillation

KW - atrial natriuretic peptide

KW - chromogranin A

KW - heart

KW - secretion

U2 - 10.2217/bmm-2017-0052

DO - 10.2217/bmm-2017-0052

M3 - Letter

C2 - 28685598

AN - SCOPUS:85024365840

SN - 1752-0363

VL - 11

SP - 541

EP - 545

JO - Biomarkers in Medicine

JF - Biomarkers in Medicine

IS - 7

ER -

Chromogranin A in the mammalian heart: Expression without secretion

Abstract

Keywords

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