CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication

Jan Dettmer; Robertas Ursache; Ana Campilho; Shunsuke Miyashima; Ilya Belevich; Seana O'Regan; Daniel Leroy Mullendore; Shri Ram Yadav; Christa Lanz; Luca Beverina; Antonio Papagni; Korbinian Schneeberger; Detlef Weigel; York-Dieter Stierhof; Thomas Moritz; Michael Knoblauch; Eija Jokitalo; Yka Helariutta

doi:10.1038/ncomms5276

CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication

Jan Dettmer, Robertas Ursache, Ana Campilho, Shunsuke Miyashima, Ilya Belevich, Seana O'Regan, Daniel Leroy Mullendore, Shri Ram Yadav, Christa Lanz, Luca Beverina, Antonio Papagni, Korbinian Schneeberger, Detlef Weigel, York-Dieter Stierhof, Thomas Moritz, Michael Knoblauch, Eija Jokitalo, Yka Helariutta

44 Citations (Scopus)

Abstract

Phloem, a plant tissue responsible for long-distance molecular transport, harbours specific junctions, sieve areas, between the conducting cells. To date, little is known about the molecular framework related to the biogenesis of these sieve areas. Here we identify mutations at the CHER1/AtCTL1 locus of Arabidopsis thaliana. The mutations cause several phenotypic abnormalities, including reduced pore density and altered pore structure in the sieve areas associated with impaired phloem function. CHER1 encodes a member of a poorly characterized choline transporter-like protein family in plants and animals. We show that CHER1 facilitates choline transport, localizes to the trans-Golgi network, and during cytokinesis is associated with the phragmoplast. Consistent with its function in the elaboration of the sieve areas, CHER1 has a sustained, polar localization in the forming sieve plates. Our results indicate that the regulation of choline levels is crucial for phloem development and conductivity in plants.

Original language	English
Journal	Nature Communications
Volume	5
ISSN	2041-1723
DOIs	https://doi.org/10.1038/ncomms5276
Publication status	Published - 10 Jul 2014
Externally published	Yes

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Dettmer, J., Ursache, R., Campilho, A., Miyashima, S., Belevich, I., O'Regan, S., Mullendore, D. L., Yadav, S. R., Lanz, C., Beverina, L., Papagni, A., Schneeberger, K., Weigel, D., Stierhof, Y-D., Moritz, T., Knoblauch, M., Jokitalo, E., & Helariutta, Y. (2014). CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication. Nature Communications, 5. https://doi.org/10.1038/ncomms5276

Dettmer, J, Ursache, R, Campilho, A, Miyashima, S, Belevich, I, O'Regan, S, Mullendore, DL, Yadav, SR, Lanz, C, Beverina, L, Papagni, A, Schneeberger, K, Weigel, D, Stierhof, Y-D, Moritz, T, Knoblauch, M, Jokitalo, E & Helariutta, Y 2014, 'CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication', Nature Communications, vol. 5. https://doi.org/10.1038/ncomms5276

@article{e958973e46574fd2bd8fbda8dab3f189,

title = "CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication",

abstract = "Phloem, a plant tissue responsible for long-distance molecular transport, harbours specific junctions, sieve areas, between the conducting cells. To date, little is known about the molecular framework related to the biogenesis of these sieve areas. Here we identify mutations at the CHER1/AtCTL1 locus of Arabidopsis thaliana. The mutations cause several phenotypic abnormalities, including reduced pore density and altered pore structure in the sieve areas associated with impaired phloem function. CHER1 encodes a member of a poorly characterized choline transporter-like protein family in plants and animals. We show that CHER1 facilitates choline transport, localizes to the trans-Golgi network, and during cytokinesis is associated with the phragmoplast. Consistent with its function in the elaboration of the sieve areas, CHER1 has a sustained, polar localization in the forming sieve plates. Our results indicate that the regulation of choline levels is crucial for phloem development and conductivity in plants.",

author = "Jan Dettmer and Robertas Ursache and Ana Campilho and Shunsuke Miyashima and Ilya Belevich and Seana O'Regan and Mullendore, {Daniel Leroy} and Yadav, {Shri Ram} and Christa Lanz and Luca Beverina and Antonio Papagni and Korbinian Schneeberger and Detlef Weigel and York-Dieter Stierhof and Thomas Moritz and Michael Knoblauch and Eija Jokitalo and Yka Helariutta",

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doi = "10.1038/ncomms5276",

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T1 - CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication

AU - Dettmer, Jan

AU - Ursache, Robertas

AU - Campilho, Ana

AU - Miyashima, Shunsuke

AU - Belevich, Ilya

AU - O'Regan, Seana

AU - Mullendore, Daniel Leroy

AU - Yadav, Shri Ram

AU - Lanz, Christa

AU - Beverina, Luca

AU - Papagni, Antonio

AU - Schneeberger, Korbinian

AU - Weigel, Detlef

AU - Stierhof, York-Dieter

AU - Moritz, Thomas

AU - Knoblauch, Michael

AU - Jokitalo, Eija

AU - Helariutta, Yka

PY - 2014/7/10

Y1 - 2014/7/10

N2 - Phloem, a plant tissue responsible for long-distance molecular transport, harbours specific junctions, sieve areas, between the conducting cells. To date, little is known about the molecular framework related to the biogenesis of these sieve areas. Here we identify mutations at the CHER1/AtCTL1 locus of Arabidopsis thaliana. The mutations cause several phenotypic abnormalities, including reduced pore density and altered pore structure in the sieve areas associated with impaired phloem function. CHER1 encodes a member of a poorly characterized choline transporter-like protein family in plants and animals. We show that CHER1 facilitates choline transport, localizes to the trans-Golgi network, and during cytokinesis is associated with the phragmoplast. Consistent with its function in the elaboration of the sieve areas, CHER1 has a sustained, polar localization in the forming sieve plates. Our results indicate that the regulation of choline levels is crucial for phloem development and conductivity in plants.

AB - Phloem, a plant tissue responsible for long-distance molecular transport, harbours specific junctions, sieve areas, between the conducting cells. To date, little is known about the molecular framework related to the biogenesis of these sieve areas. Here we identify mutations at the CHER1/AtCTL1 locus of Arabidopsis thaliana. The mutations cause several phenotypic abnormalities, including reduced pore density and altered pore structure in the sieve areas associated with impaired phloem function. CHER1 encodes a member of a poorly characterized choline transporter-like protein family in plants and animals. We show that CHER1 facilitates choline transport, localizes to the trans-Golgi network, and during cytokinesis is associated with the phragmoplast. Consistent with its function in the elaboration of the sieve areas, CHER1 has a sustained, polar localization in the forming sieve plates. Our results indicate that the regulation of choline levels is crucial for phloem development and conductivity in plants.

U2 - 10.1038/ncomms5276

DO - 10.1038/ncomms5276

M3 - Journal article

SN - 2041-1723

VL - 5

JO - Nature Communications

JF - Nature Communications

ER -

CHOLINE TRANSPORTER-LIKE1 is required for sieve plate development to mediate long-distance cell-to-cell communication

Abstract

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