Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection

Rita Sousa-Nunes; Amer Ahmed Rana; Ross Kettleborough; Joshua M Brickman; Melanie Clements; Alistair Forrest; Sean Grimmond; Philip Avner; James C Smith; Sally L Dunwoodie; Rosa S P Beddington

doi:10.1101/gr.1362303

Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection

Rita Sousa-Nunes, Amer Ahmed Rana, Ross Kettleborough, Joshua M Brickman, Melanie Clements, Alistair Forrest, Sean Grimmond, Philip Avner, James C Smith, Sally L Dunwoodie, Rosa S P Beddington

Stem Cell and developmental Biology - early embryonic lineage specification Lab

24 Citations (Scopus)

Abstract

This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

Original language	English
Journal	Genome Research
Volume	13
Issue number	12
Pages (from-to)	2609-20
Number of pages	12
ISSN	1088-9051
DOIs	https://doi.org/10.1101/gr.1362303
Publication status	Published - Dec 2003

Keywords

Animals
Crosses, Genetic
DNA, Complementary
Endoderm
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Library
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Sequence Analysis, DNA

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10.1101/gr.1362303

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title = "Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection",

abstract = "This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.",

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author = "Rita Sousa-Nunes and Rana, {Amer Ahmed} and Ross Kettleborough and Brickman, {Joshua M} and Melanie Clements and Alistair Forrest and Sean Grimmond and Philip Avner and Smith, {James C} and Dunwoodie, {Sally L} and Beddington, {Rosa S P}",

year = "2003",

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doi = "10.1101/gr.1362303",

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AU - Sousa-Nunes, Rita

AU - Rana, Amer Ahmed

AU - Kettleborough, Ross

AU - Brickman, Joshua M

AU - Clements, Melanie

AU - Forrest, Alistair

AU - Grimmond, Sean

AU - Avner, Philip

AU - Smith, James C

AU - Dunwoodie, Sally L

AU - Beddington, Rosa S P

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N2 - This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

AB - This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node-tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

KW - Animals

KW - Crosses, Genetic

KW - DNA, Complementary

KW - Endoderm

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Developmental

KW - Gene Library

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Inbred CBA

KW - Sequence Analysis, DNA

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DO - 10.1101/gr.1362303

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JF - Genome Research

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Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection

Abstract

Keywords

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