Characterization of two novel cutaneous human papillomaviruses, HPV93 and HPV96

Natasa Vasiljevic; Kristina Hazard; Linda Eliasson; Hoang Ly; Andreas Hunziker; Ethel-Michele de Villiers; Bodil Norrild; Joakim Dillner; Ola Forslund

doi:10.1099/vir.0.82679-0

Characterization of two novel cutaneous human papillomaviruses, HPV93 and HPV96

Natasa Vasiljevic, Kristina Hazard, Linda Eliasson, Hoang Ly, Andreas Hunziker, Ethel-Michele de Villiers, Bodil Norrild, Joakim Dillner, Ola Forslund

Department of Cellular and Molecular Medicine

28 Citations (Scopus)

Abstract

Two novel human papillomaviruses (HPVs), HPV93 and HPV96, with genomes of 7450 and 7438 bp, respectively, are described. The L1 open reading frame of HPV93 showed highest identity to HPV24 (79%) and that of HPV96 had highest identity to HPV92 (71%). Real-time PCR for HPV92, 93 and 96 on stripped biopsies from tumours and healthy skin from 269 immunocompetent patients found HPV DNA in 2.6% of tumours and in 0.4% of healthy skin samples. Double infections were observed in two tumours. HPV92 was detected in four, HPV93 in two and HPV96 in three tumours. The range of viral loads spanned from one copy per 45 cells to one copy per 10,000 cells. The E7 proteins of HPV92, 93 and 96 were found to bind the retinoblastoma protein (pRb). These results suggest a possible role for these HPV types in skin carcinogenesis that deserves further study.

Original language	English
Journal	Journal of General Virology
Volume	88
Issue number	Pt 5
Pages (from-to)	1479-83
Number of pages	4
ISSN	0022-1317
DOIs	https://doi.org/10.1099/vir.0.82679-0
Publication status	Published - 2007

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@article{e505d010a91e11debc73000ea68e967b,

title = "Characterization of two novel cutaneous human papillomaviruses, HPV93 and HPV96",

abstract = "Two novel human papillomaviruses (HPVs), HPV93 and HPV96, with genomes of 7450 and 7438 bp, respectively, are described. The L1 open reading frame of HPV93 showed highest identity to HPV24 (79%) and that of HPV96 had highest identity to HPV92 (71%). Real-time PCR for HPV92, 93 and 96 on stripped biopsies from tumours and healthy skin from 269 immunocompetent patients found HPV DNA in 2.6% of tumours and in 0.4% of healthy skin samples. Double infections were observed in two tumours. HPV92 was detected in four, HPV93 in two and HPV96 in three tumours. The range of viral loads spanned from one copy per 45 cells to one copy per 10,000 cells. The E7 proteins of HPV92, 93 and 96 were found to bind the retinoblastoma protein (pRb). These results suggest a possible role for these HPV types in skin carcinogenesis that deserves further study.",

author = "Natasa Vasiljevic and Kristina Hazard and Linda Eliasson and Hoang Ly and Andreas Hunziker and {de Villiers}, Ethel-Michele and Bodil Norrild and Joakim Dillner and Ola Forslund",

note = "Keywords: DNA Primers; DNA, Viral; Genome, Viral; Humans; Molecular Sequence Data; Open Reading Frames; Papillomaviridae; RNA, Messenger; RNA, Viral; Reference Values; Skin",

year = "2007",

doi = "10.1099/vir.0.82679-0",

language = "English",

volume = "88",

pages = "1479--83",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Society for General Microbiology",

number = "Pt 5",

}

TY - JOUR

T1 - Characterization of two novel cutaneous human papillomaviruses, HPV93 and HPV96

AU - Vasiljevic, Natasa

AU - Hazard, Kristina

AU - Eliasson, Linda

AU - Ly, Hoang

AU - Hunziker, Andreas

AU - de Villiers, Ethel-Michele

AU - Norrild, Bodil

AU - Dillner, Joakim

AU - Forslund, Ola

N1 - Keywords: DNA Primers; DNA, Viral; Genome, Viral; Humans; Molecular Sequence Data; Open Reading Frames; Papillomaviridae; RNA, Messenger; RNA, Viral; Reference Values; Skin

PY - 2007

Y1 - 2007

N2 - Two novel human papillomaviruses (HPVs), HPV93 and HPV96, with genomes of 7450 and 7438 bp, respectively, are described. The L1 open reading frame of HPV93 showed highest identity to HPV24 (79%) and that of HPV96 had highest identity to HPV92 (71%). Real-time PCR for HPV92, 93 and 96 on stripped biopsies from tumours and healthy skin from 269 immunocompetent patients found HPV DNA in 2.6% of tumours and in 0.4% of healthy skin samples. Double infections were observed in two tumours. HPV92 was detected in four, HPV93 in two and HPV96 in three tumours. The range of viral loads spanned from one copy per 45 cells to one copy per 10,000 cells. The E7 proteins of HPV92, 93 and 96 were found to bind the retinoblastoma protein (pRb). These results suggest a possible role for these HPV types in skin carcinogenesis that deserves further study.

AB - Two novel human papillomaviruses (HPVs), HPV93 and HPV96, with genomes of 7450 and 7438 bp, respectively, are described. The L1 open reading frame of HPV93 showed highest identity to HPV24 (79%) and that of HPV96 had highest identity to HPV92 (71%). Real-time PCR for HPV92, 93 and 96 on stripped biopsies from tumours and healthy skin from 269 immunocompetent patients found HPV DNA in 2.6% of tumours and in 0.4% of healthy skin samples. Double infections were observed in two tumours. HPV92 was detected in four, HPV93 in two and HPV96 in three tumours. The range of viral loads spanned from one copy per 45 cells to one copy per 10,000 cells. The E7 proteins of HPV92, 93 and 96 were found to bind the retinoblastoma protein (pRb). These results suggest a possible role for these HPV types in skin carcinogenesis that deserves further study.

U2 - 10.1099/vir.0.82679-0

DO - 10.1099/vir.0.82679-0

M3 - Journal article

C2 - 17412976

SN - 0022-1317

VL - 88

SP - 1479

EP - 1483

JO - Journal of General Virology

JF - Journal of General Virology

IS - Pt 5

ER -

Characterization of two novel cutaneous human papillomaviruses, HPV93 and HPV96

Abstract

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