Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-D-galactotetraose

Mathias C.F. Andersen; Irene Boos; Susan E. Marcus; Stjepan Kresimir Kracun; Maja Gro Rydahl; William George Tycho Willats; J. Paul Knox; Mads H. Clausen

doi:10.1016/j.carres.2016.10.012

Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-D-galactotetraose

Mathias C.F. Andersen, Irene Boos, Susan E. Marcus, Stjepan Kresimir Kracun, Maja Gro Rydahl, William George Tycho Willats, J. Paul Knox, Mads H. Clausen^*

^*Corresponding author for this work

Section for Plant Glycobiology

15 Citations (Scopus)

Abstract

Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies (mAbs) for localization and visualization of glycans, using e.g. immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mAb LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogues of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.

Original language	English
Journal	Carbohydrate Research
Volume	436
Pages (from-to)	36-40
Number of pages	5
ISSN	0008-6215
DOIs	https://doi.org/10.1016/j.carres.2016.10.012
Publication status	Published - 2016

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10.1016/j.carres.2016.10.012

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@article{3427c3c2ceff45bdbd245730b3246cf8,

title = "Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-D-galactotetraose",

abstract = "Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies (mAbs) for localization and visualization of glycans, using e.g. immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mAb LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogues of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.",

author = "Andersen, {Mathias C.F.} and Irene Boos and Marcus, {Susan E.} and Kracun, {Stjepan Kresimir} and Rydahl, {Maja Gro} and Willats, {William George Tycho} and Knox, {J. Paul} and Clausen, {Mads H.}",

year = "2016",

doi = "10.1016/j.carres.2016.10.012",

language = "English",

volume = "436",

pages = "36--40",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Pergamon Press",

}

TY - JOUR

T1 - Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-D-galactotetraose

AU - Andersen, Mathias C.F.

AU - Boos, Irene

AU - Marcus, Susan E.

AU - Kracun, Stjepan Kresimir

AU - Rydahl, Maja Gro

AU - Willats, William George Tycho

AU - Knox, J. Paul

AU - Clausen, Mads H.

PY - 2016

Y1 - 2016

N2 - Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies (mAbs) for localization and visualization of glycans, using e.g. immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mAb LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogues of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.

AB - Plant cell wall glycans are important polymers that are crucial to plant development and serve as an important source of sustainable biomass. The study of polysaccharides in the plant cell wall relies heavily on monoclonal antibodies (mAbs) for localization and visualization of glycans, using e.g. immunofluorescent microscopy. Here, we describe the detailed epitope mapping of the mAb LM5 that is shown to bind to a minimum of three sugar residues at the non-reducing end of linear beta-1,4-linked galactan. The study uses de novo synthetic analogues of galactans combined with carbohydrate microarray and competitive inhibition ELISA for analysis of antibody-carbohydrate interactions.

U2 - 10.1016/j.carres.2016.10.012

DO - 10.1016/j.carres.2016.10.012

M3 - Journal article

C2 - 27855335

AN - SCOPUS:84995662311

SN - 0008-6215

VL - 436

SP - 36

EP - 40

JO - Carbohydrate Research

JF - Carbohydrate Research

ER -

Characterization of the LM5 pectic galactan epitope with synthetic analogues of β-1,4-D-galactotetraose

Abstract

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