Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines.

TY - JOUR

T1 - Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines.

AU - Nielsen, Sonja K

AU - Møllgård, Kjeld

AU - Clement, Christian A

AU - Veland, Iben R

AU - Awan, Aashir

AU - Yoder, Bradley K

AU - Novak, Ivana

AU - Christensen, Søren Tvorup

N1 - Keywords: Adenocarcinoma; Animals; Carcinoma, Pancreatic Ductal; Cell Line, Tumor; Cilia; Epithelial Cells; Female; Fetus; Green Fluorescent Proteins; Hedgehog Proteins; Humans; Kruppel-Like Transcription Factors; Mice; NIH 3T3 Cells; Nuclear Proteins; Pancreas; Pancreatic Neoplasms; Pregnancy; Receptors, G-Protein-Coupled; Transfection

PY - 2008

Y1 - 2008

N2 - Hedgehog (Hh) signaling controls pancreatic development and homeostasis; aberrant Hh signaling is associated with several pancreatic diseases. Here we investigated the link between Hh signaling and primary cilia in the human developing pancreatic ducts and in cultures of human pancreatic duct adenocarcinoma cell lines, PANC-1 and CFPAC-1. We show that the onset of Hh signaling from human embryogenesis to fetal development is associated with accumulation of Hh signaling components Smo and Gli2 in duct primary cilia and a reduction of Gli3 in the duct epithelium. Smo, Ptc, and Gli2 localized to primary cilia of PANC-1 and CFPAC-1 cells, which may maintain high levels of nonstimulated Hh pathway activity. These findings indicate that primary cilia are involved in pancreatic development and postnatal tissue homeostasis.

AB - Hedgehog (Hh) signaling controls pancreatic development and homeostasis; aberrant Hh signaling is associated with several pancreatic diseases. Here we investigated the link between Hh signaling and primary cilia in the human developing pancreatic ducts and in cultures of human pancreatic duct adenocarcinoma cell lines, PANC-1 and CFPAC-1. We show that the onset of Hh signaling from human embryogenesis to fetal development is associated with accumulation of Hh signaling components Smo and Gli2 in duct primary cilia and a reduction of Gli3 in the duct epithelium. Smo, Ptc, and Gli2 localized to primary cilia of PANC-1 and CFPAC-1 cells, which may maintain high levels of nonstimulated Hh pathway activity. These findings indicate that primary cilia are involved in pancreatic development and postnatal tissue homeostasis.

U2 - 10.1002/dvdy.21610

DO - 10.1002/dvdy.21610

M3 - Journal article

C2 - 18629868

SN - 1058-8388

VL - 237

SP - 2039

EP - 2052

JO - Developmental Dynamics

JF - Developmental Dynamics

IS - 8

ER -