Characterization of murine melanocortin receptors mediating adipocyte lipolysis and examination of signalling pathways involved

Cathrine Laustrup Møller; Kirsten Raun; Marianne Lambert Jacobsen; Thomas Åskov Pedersen; Birgitte Holst; Kilian W Conde-Frieboes; Birgitte Schjellerup Wulff

doi:10.1016/j.mce.2011.03.010

Characterization of murine melanocortin receptors mediating adipocyte lipolysis and examination of signalling pathways involved

Cathrine Laustrup Møller, Kirsten Raun, Marianne Lambert Jacobsen, Thomas Åskov Pedersen, Birgitte Holst, Kilian W Conde-Frieboes, Birgitte Schjellerup Wulff

28 Citations (Scopus)

Abstract

The melanocortin receptors (MCRs) belong to the G-protein coupled receptors (family A). So far, 5 different subtypes have been described (MC1R-MC5R) and of these MC2R and MC5R have been proposed to act directly in adipocytes and regulate lipolysis in rodents. Using ACTH and α-melanocyte stimulating hormone (α-MSH) generated from proopiomelanocortin (POMC), as well as synthetic MSH analogues to stimulate lipolysis in murine 3T3-L1 adipocytes it is shown that MC2R and MC5R are lipolytic mediators in differentiated 3T3-L1 adipocytes. Involvement of cAMP, phosphorylated extracellular signal-regulated kinase (ERK) 1/2, protein kinase B (PKB), adenosine 5′ monophosphate activated protein kinase (AMPK) and Jun-amino-terminal kinase (JNK) in MCR mediated lipolysis were studied. Interestingly, results obtained in 3T3-L1 cells suggest that lipolysis stimulated by α-MSH, NDP-α-MSH, MT-II, SHU9119 and PG-901 is mediated through MC5R in a cAMP independent manner. Finally, we identify essential differences in MCR mediated lipolysis when using 3T3-L1 cells compared to primary adipocytes.

Original language	English
Journal	Molecular and Cellular Endocrinology
Volume	341
Issue number	1-2
Pages (from-to)	9-17
Number of pages	9
ISSN	0303-7207
DOIs	https://doi.org/10.1016/j.mce.2011.03.010
Publication status	Published - 20 Jul 2011

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Characterization of murine melanocortin receptors mediating adipocyte lipolysis and examination of signalling pathways involved. / Møller, Cathrine Laustrup; Raun, Kirsten; Jacobsen, Marianne Lambert et al.

In: Molecular and Cellular Endocrinology, Vol. 341, No. 1-2, 20.07.2011, p. 9-17.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Characterization of murine melanocortin receptors mediating adipocyte lipolysis and examination of signalling pathways involved",

abstract = "The melanocortin receptors (MCRs) belong to the G-protein coupled receptors (family A). So far, 5 different subtypes have been described (MC1R-MC5R) and of these MC2R and MC5R have been proposed to act directly in adipocytes and regulate lipolysis in rodents. Using ACTH and α-melanocyte stimulating hormone (α-MSH) generated from proopiomelanocortin (POMC), as well as synthetic MSH analogues to stimulate lipolysis in murine 3T3-L1 adipocytes it is shown that MC2R and MC5R are lipolytic mediators in differentiated 3T3-L1 adipocytes. Involvement of cAMP, phosphorylated extracellular signal-regulated kinase (ERK) 1/2, protein kinase B (PKB), adenosine 5′ monophosphate activated protein kinase (AMPK) and Jun-amino-terminal kinase (JNK) in MCR mediated lipolysis were studied. Interestingly, results obtained in 3T3-L1 cells suggest that lipolysis stimulated by α-MSH, NDP-α-MSH, MT-II, SHU9119 and PG-901 is mediated through MC5R in a cAMP independent manner. Finally, we identify essential differences in MCR mediated lipolysis when using 3T3-L1 cells compared to primary adipocytes.",

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T1 - Characterization of murine melanocortin receptors mediating adipocyte lipolysis and examination of signalling pathways involved

AU - Møller, Cathrine Laustrup

AU - Raun, Kirsten

AU - Jacobsen, Marianne Lambert

AU - Pedersen, Thomas Åskov

AU - Holst, Birgitte

AU - Conde-Frieboes, Kilian W

AU - Wulff, Birgitte Schjellerup

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N2 - The melanocortin receptors (MCRs) belong to the G-protein coupled receptors (family A). So far, 5 different subtypes have been described (MC1R-MC5R) and of these MC2R and MC5R have been proposed to act directly in adipocytes and regulate lipolysis in rodents. Using ACTH and α-melanocyte stimulating hormone (α-MSH) generated from proopiomelanocortin (POMC), as well as synthetic MSH analogues to stimulate lipolysis in murine 3T3-L1 adipocytes it is shown that MC2R and MC5R are lipolytic mediators in differentiated 3T3-L1 adipocytes. Involvement of cAMP, phosphorylated extracellular signal-regulated kinase (ERK) 1/2, protein kinase B (PKB), adenosine 5′ monophosphate activated protein kinase (AMPK) and Jun-amino-terminal kinase (JNK) in MCR mediated lipolysis were studied. Interestingly, results obtained in 3T3-L1 cells suggest that lipolysis stimulated by α-MSH, NDP-α-MSH, MT-II, SHU9119 and PG-901 is mediated through MC5R in a cAMP independent manner. Finally, we identify essential differences in MCR mediated lipolysis when using 3T3-L1 cells compared to primary adipocytes.

AB - The melanocortin receptors (MCRs) belong to the G-protein coupled receptors (family A). So far, 5 different subtypes have been described (MC1R-MC5R) and of these MC2R and MC5R have been proposed to act directly in adipocytes and regulate lipolysis in rodents. Using ACTH and α-melanocyte stimulating hormone (α-MSH) generated from proopiomelanocortin (POMC), as well as synthetic MSH analogues to stimulate lipolysis in murine 3T3-L1 adipocytes it is shown that MC2R and MC5R are lipolytic mediators in differentiated 3T3-L1 adipocytes. Involvement of cAMP, phosphorylated extracellular signal-regulated kinase (ERK) 1/2, protein kinase B (PKB), adenosine 5′ monophosphate activated protein kinase (AMPK) and Jun-amino-terminal kinase (JNK) in MCR mediated lipolysis were studied. Interestingly, results obtained in 3T3-L1 cells suggest that lipolysis stimulated by α-MSH, NDP-α-MSH, MT-II, SHU9119 and PG-901 is mediated through MC5R in a cAMP independent manner. Finally, we identify essential differences in MCR mediated lipolysis when using 3T3-L1 cells compared to primary adipocytes.

U2 - 10.1016/j.mce.2011.03.010

DO - 10.1016/j.mce.2011.03.010

M3 - Journal article

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JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

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ER -

Characterization of murine melanocortin receptors mediating adipocyte lipolysis and examination of signalling pathways involved

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