Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.

Lotte Bang Pedersen; T Murray; D L Popham; P Setlow

Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.

Lotte Bang Pedersen, T Murray, D L Popham, P Setlow

Cell Biology and Physiology

28 Citations (Scopus)

Abstract

The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed.
Udgivelsesdato: 1998-Sep

Original language	English
Journal	Journal of Bacteriology
Volume	180
Issue number	18
Pages (from-to)	4967-73
Number of pages	6
ISSN	0021-9193
Publication status	Published - 1998

Cite this

@article{44873ad0e07911dcbee902004c4f4f50,

title = "Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.",

abstract = "The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed. Udgivelsesdato: 1998-Sep",

author = "Pedersen, {Lotte Bang} and T Murray and Popham, {D L} and P Setlow",

note = "Keywords: Bacillus subtilis; Bacterial Proteins; Carrier Proteins; Escherichia coli; Genes, Bacterial; Hexosyltransferases; Lac Operon; Molecular Weight; Muramoylpentapeptide Carboxypeptidase; Mutation; Penicillin-Binding Proteins; Peptidyl Transferases; Transcription, Genetic",

year = "1998",

language = "English",

volume = "180",

pages = "4967--73",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "18",

}

TY - JOUR

T1 - Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.

AU - Pedersen, Lotte Bang

AU - Murray, T

AU - Popham, D L

AU - Setlow, P

N1 - Keywords: Bacillus subtilis; Bacterial Proteins; Carrier Proteins; Escherichia coli; Genes, Bacterial; Hexosyltransferases; Lac Operon; Molecular Weight; Muramoylpentapeptide Carboxypeptidase; Mutation; Penicillin-Binding Proteins; Peptidyl Transferases; Transcription, Genetic

PY - 1998

Y1 - 1998

N2 - The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed. Udgivelsesdato: 1998-Sep

AB - The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed. Udgivelsesdato: 1998-Sep

M3 - Journal article

C2 - 9733705

SN - 0021-9193

VL - 180

SP - 4967

EP - 4973

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 18

ER -