Characterization of an ex vivo femoral head model assessed by markers of bone and cartilage turnover

Suzi Hoegh Madsen; Anne Sofie Goettrup; Gedske  Thomsen; Søren Tvorup Christensen; Nikolaj Schultz; Kim Henriksen; Anne-Christine Bay-Jensen; Morten Asser Karsdal

doi:10.1177/1947603510383855

Characterization of an ex vivo femoral head model assessed by markers of bone and cartilage turnover

Suzi Hoegh Madsen, Anne Sofie Goettrup, Gedske Thomsen, Søren Tvorup Christensen, Nikolaj Schultz, Kim Henriksen, Anne-Christine Bay-Jensen, Morten Asser Karsdal

Cell Biology and Physiology

10 Citations (Scopus)

Abstract

Objective: The pathophysiology of osteoarthritis involves the whole joint and is characterized by cartilage degradation and altered subchondral bone turnover. At present, there is a need for biological models that allow investigation of the interactions between the key cellular players in bone/cartilage: osteoblasts, osteoclasts, and chondrocytes. Methods: Femoral heads from 3-, 6-, 9-, and 12-week-old female mice were isolated and cultured for 10 days in serum-free media in the absence or presence of IGF-I (100 nM) (anabolic stimulation) or OSM (10 ng/mL) + TNF-α (20 ng/mL) (catabolic stimulation). Histology on femoral heads before and after culture was performed, and the growth plate size was examined to evaluate the effects on cell metabolism. The conditioned medium was examined for biochemical markers of bone and cartilage degradation/ formation. Results: Each age group represented a unique system regarding the interest of bone or cartilage metabolism. Stimulation over 10 days with OSM + TNF-α resulted in depletion of proteoglycans from the cartilage surface in all ages. Furthermore, OSM + TNF-α decreased growth plate size, whereas IGF-I increased the size. Measurements from the conditioned media showed that OSM + TNF-α increased the number of osteoclasts by approximately 80% and induced bone and cartilage degradation by approximately 1200% and approximately 2600%, respectively. Stimulation with IGF-I decreased the osteoclast number and increased cartilage formation by approximately 30%. Conclusion: Biochemical markers and histology together showed that the catabolic stimulation induced degradation and the anabolic stimulation induced formation in the femoral heads. We propose that we have established an explant whole-tissue model for investigating cellcell interactions, reflecting parts of the processes in the pathogenesis of joint degenerative diseases.

Original language	English
Journal	Cartilage
Volume	2
Issue number	3
Pages (from-to)	265-278
Number of pages	14
DOIs	https://doi.org/10.1177/1947603510383855
Publication status	Published - Jul 2011

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10.1177/1947603510383855

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@article{209f5bb4891644b8b3de32f386eff002,

title = "Characterization of an ex vivo femoral head model assessed by markers of bone and cartilage turnover",

abstract = "Objective: The pathophysiology of osteoarthritis involves the whole joint and is characterized by cartilage degradation and altered subchondral bone turnover. At present, there is a need for biological models that allow investigation of the interactions between the key cellular players in bone/cartilage: osteoblasts, osteoclasts, and chondrocytes. Methods: Femoral heads from 3-, 6-, 9-, and 12-week-old female mice were isolated and cultured for 10 days in serum-free media in the absence or presence of IGF-I (100 nM) (anabolic stimulation) or OSM (10 ng/mL) + TNF-α (20 ng/mL) (catabolic stimulation). Histology on femoral heads before and after culture was performed, and the growth plate size was examined to evaluate the effects on cell metabolism. The conditioned medium was examined for biochemical markers of bone and cartilage degradation/ formation. Results: Each age group represented a unique system regarding the interest of bone or cartilage metabolism. Stimulation over 10 days with OSM + TNF-α resulted in depletion of proteoglycans from the cartilage surface in all ages. Furthermore, OSM + TNF-α decreased growth plate size, whereas IGF-I increased the size. Measurements from the conditioned media showed that OSM + TNF-α increased the number of osteoclasts by approximately 80% and induced bone and cartilage degradation by approximately 1200% and approximately 2600%, respectively. Stimulation with IGF-I decreased the osteoclast number and increased cartilage formation by approximately 30%. Conclusion: Biochemical markers and histology together showed that the catabolic stimulation induced degradation and the anabolic stimulation induced formation in the femoral heads. We propose that we have established an explant whole-tissue model for investigating cellcell interactions, reflecting parts of the processes in the pathogenesis of joint degenerative diseases.",

author = "Madsen, {Suzi Hoegh} and Goettrup, {Anne Sofie} and Gedske Thomsen and Christensen, {S{\o}ren Tvorup} and Nikolaj Schultz and Kim Henriksen and Anne-Christine Bay-Jensen and Karsdal, {Morten Asser}",

year = "2011",

month = jul,

doi = "10.1177/1947603510383855",

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pages = "265--278",

journal = "Cartilage",

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T1 - Characterization of an ex vivo femoral head model assessed by markers of bone and cartilage turnover

AU - Madsen, Suzi Hoegh

AU - Goettrup, Anne Sofie

AU - Thomsen, Gedske

AU - Christensen, Søren Tvorup

AU - Schultz, Nikolaj

AU - Henriksen, Kim

AU - Bay-Jensen, Anne-Christine

AU - Karsdal, Morten Asser

PY - 2011/7

Y1 - 2011/7

N2 - Objective: The pathophysiology of osteoarthritis involves the whole joint and is characterized by cartilage degradation and altered subchondral bone turnover. At present, there is a need for biological models that allow investigation of the interactions between the key cellular players in bone/cartilage: osteoblasts, osteoclasts, and chondrocytes. Methods: Femoral heads from 3-, 6-, 9-, and 12-week-old female mice were isolated and cultured for 10 days in serum-free media in the absence or presence of IGF-I (100 nM) (anabolic stimulation) or OSM (10 ng/mL) + TNF-α (20 ng/mL) (catabolic stimulation). Histology on femoral heads before and after culture was performed, and the growth plate size was examined to evaluate the effects on cell metabolism. The conditioned medium was examined for biochemical markers of bone and cartilage degradation/ formation. Results: Each age group represented a unique system regarding the interest of bone or cartilage metabolism. Stimulation over 10 days with OSM + TNF-α resulted in depletion of proteoglycans from the cartilage surface in all ages. Furthermore, OSM + TNF-α decreased growth plate size, whereas IGF-I increased the size. Measurements from the conditioned media showed that OSM + TNF-α increased the number of osteoclasts by approximately 80% and induced bone and cartilage degradation by approximately 1200% and approximately 2600%, respectively. Stimulation with IGF-I decreased the osteoclast number and increased cartilage formation by approximately 30%. Conclusion: Biochemical markers and histology together showed that the catabolic stimulation induced degradation and the anabolic stimulation induced formation in the femoral heads. We propose that we have established an explant whole-tissue model for investigating cellcell interactions, reflecting parts of the processes in the pathogenesis of joint degenerative diseases.

AB - Objective: The pathophysiology of osteoarthritis involves the whole joint and is characterized by cartilage degradation and altered subchondral bone turnover. At present, there is a need for biological models that allow investigation of the interactions between the key cellular players in bone/cartilage: osteoblasts, osteoclasts, and chondrocytes. Methods: Femoral heads from 3-, 6-, 9-, and 12-week-old female mice were isolated and cultured for 10 days in serum-free media in the absence or presence of IGF-I (100 nM) (anabolic stimulation) or OSM (10 ng/mL) + TNF-α (20 ng/mL) (catabolic stimulation). Histology on femoral heads before and after culture was performed, and the growth plate size was examined to evaluate the effects on cell metabolism. The conditioned medium was examined for biochemical markers of bone and cartilage degradation/ formation. Results: Each age group represented a unique system regarding the interest of bone or cartilage metabolism. Stimulation over 10 days with OSM + TNF-α resulted in depletion of proteoglycans from the cartilage surface in all ages. Furthermore, OSM + TNF-α decreased growth plate size, whereas IGF-I increased the size. Measurements from the conditioned media showed that OSM + TNF-α increased the number of osteoclasts by approximately 80% and induced bone and cartilage degradation by approximately 1200% and approximately 2600%, respectively. Stimulation with IGF-I decreased the osteoclast number and increased cartilage formation by approximately 30%. Conclusion: Biochemical markers and histology together showed that the catabolic stimulation induced degradation and the anabolic stimulation induced formation in the femoral heads. We propose that we have established an explant whole-tissue model for investigating cellcell interactions, reflecting parts of the processes in the pathogenesis of joint degenerative diseases.

U2 - 10.1177/1947603510383855

DO - 10.1177/1947603510383855

M3 - Journal article

C2 - 26069585

SN - 1947-6043

VL - 2

SP - 265

EP - 278

JO - Cartilage

JF - Cartilage

IS - 3

ER -

Characterization of an ex vivo femoral head model assessed by markers of bone and cartilage turnover

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