Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

Tomas Laursen; Jonas Borch-Jensen; Camilla Knudsen Baden; Krutika Bavishi; Federico Torta; Helle Juel Martens; Daniele Silvestro; Nikos Hatzakis; Markus R Wenk; Timothy R Dafforn; Carl Erik Olsen; Mohammed Saddik Motawie; Björn Robert Hamberger; Birger Lindberg Møller; Jean-Étienne André Bassard

doi:10.1126/science.aag2347

Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

Tomas Laursen, Jonas Borch-Jensen, Camilla Knudsen Baden, Krutika Bavishi, Federico Torta, Helle Juel Martens, Daniele Silvestro, Nikos Hatzakis, Markus R Wenk, Timothy R Dafforn, Carl Erik Olsen, Mohammed Saddik Motawie, Björn Robert Hamberger, Birger Lindberg Møller, Jean-Étienne André Bassard

122 Citations (Scopus)

Abstract

Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.

Original language	English
Journal	Science (New York, N.Y.)
Volume	354
Issue number	6314
Pages (from-to)	890-893
Number of pages	4
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.aag2347
Publication status	Published - 18 Nov 2016

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Laursen, T., Borch-Jensen, J., Baden, C. K., Bavishi, K., Torta, F., Martens, H. J., Silvestro, D., Hatzakis, N., Wenk, M. R., Dafforn, T. R., Olsen, C. E., Motawie, M. S., Hamberger, B. R., Møller, B. L., & Bassard, J.-É. A. (2016). Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum. Science (New York, N.Y.), 354(6314), 890-893. https://doi.org/10.1126/science.aag2347

Laursen, T, Borch-Jensen, J, Baden, CK, Bavishi, K, Torta, F, Martens, HJ, Silvestro, D, Hatzakis, N, Wenk, MR, Dafforn, TR, Olsen, CE, Motawie, MS, Hamberger, BR, Møller, BL & Bassard, J-ÉA 2016, 'Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum', Science (New York, N.Y.), vol. 354, no. 6314, pp. 890-893. https://doi.org/10.1126/science.aag2347

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title = "Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum",

abstract = "Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.",

author = "Tomas Laursen and Jonas Borch-Jensen and Baden, {Camilla Knudsen} and Krutika Bavishi and Federico Torta and Martens, {Helle Juel} and Daniele Silvestro and Nikos Hatzakis and Wenk, {Markus R} and Dafforn, {Timothy R} and Olsen, {Carl Erik} and Motawie, {Mohammed Saddik} and Hamberger, {Bj{\"o}rn Robert} and M{\o}ller, {Birger Lindberg} and Bassard, {Jean-{\'E}tienne Andr{\'e}}",

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doi = "10.1126/science.aag2347",

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AU - Laursen, Tomas

AU - Borch-Jensen, Jonas

AU - Baden, Camilla Knudsen

AU - Bavishi, Krutika

AU - Torta, Federico

AU - Martens, Helle Juel

AU - Silvestro, Daniele

AU - Hatzakis, Nikos

AU - Wenk, Markus R

AU - Dafforn, Timothy R

AU - Olsen, Carl Erik

AU - Motawie, Mohammed Saddik

AU - Hamberger, Björn Robert

AU - Møller, Birger Lindberg

AU - Bassard, Jean-Étienne André

PY - 2016/11/18

Y1 - 2016/11/18

N2 - Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.

AB - Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.

U2 - 10.1126/science.aag2347

DO - 10.1126/science.aag2347

M3 - Journal article

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SN - 0036-8075

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SP - 890

EP - 893

JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

IS - 6314

ER -

Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

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