Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids

Juha Matti Alakoskela; Arimatti Jutila; Adam C. Simonsen; Jussi Pirneskoski; Sinikka Pyhäjoki; Raija Turunen; Sani Marttila; Ole G. Mouritsen; Erik Goormaghtigh; Paavo K.J. Kinnunen

doi:10.1021/bi0611107

Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids

Juha Matti Alakoskela, Arimatti Jutila, Adam C. Simonsen, Jussi Pirneskoski, Sinikka Pyhäjoki, Raija Turunen, Sani Marttila, Ole G. Mouritsen, Erik Goormaghtigh, Paavo K.J. Kinnunen^*

^*Corresponding author for this work

31 Citations (Scopus)

Abstract

Recent publications described the formation of millimeter-length fibers by diverse lipid-binding proteins (e.g., histone H1, cytochrome c, indolicidin, and endostatin) when they are mixed with 80:20 phosphatidylcholine/ phosphatidylserine vesicles. Further, these fibers displayed amyloid characteristics when stained with Congo Red. In the study presented here, we found by FTIR the amide I absorption band to reveal significant variation in fibers formed by cytochrome c, with some consisting of cytochrome c in a nativelike conformation and some exhibiting strong amyloid (β-sheet) characteristics. Protein structure also varied from amyloid to nearly native within single fibers. Fibers were frequently blue or bluish and sometimes iridescent, likely due to interference of light in the fibers. The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing.

Original language	English
Journal	Biochemistry
Volume	45
Issue number	45
Pages (from-to)	13447-13453
Number of pages	7
ISSN	0006-2960
DOIs	https://doi.org/10.1021/bi0611107
Publication status	Published - 14 Nov 2006

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title = "Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids",

abstract = "Recent publications described the formation of millimeter-length fibers by diverse lipid-binding proteins (e.g., histone H1, cytochrome c, indolicidin, and endostatin) when they are mixed with 80:20 phosphatidylcholine/ phosphatidylserine vesicles. Further, these fibers displayed amyloid characteristics when stained with Congo Red. In the study presented here, we found by FTIR the amide I absorption band to reveal significant variation in fibers formed by cytochrome c, with some consisting of cytochrome c in a nativelike conformation and some exhibiting strong amyloid (β-sheet) characteristics. Protein structure also varied from amyloid to nearly native within single fibers. Fibers were frequently blue or bluish and sometimes iridescent, likely due to interference of light in the fibers. The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing.",

author = "Alakoskela, {Juha Matti} and Arimatti Jutila and Simonsen, {Adam C.} and Jussi Pirneskoski and Sinikka Pyh{\"a}joki and Raija Turunen and Sani Marttila and Mouritsen, {Ole G.} and Erik Goormaghtigh and Kinnunen, {Paavo K.J.}",

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T1 - Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids

AU - Alakoskela, Juha Matti

AU - Jutila, Arimatti

AU - Simonsen, Adam C.

AU - Pirneskoski, Jussi

AU - Pyhäjoki, Sinikka

AU - Turunen, Raija

AU - Marttila, Sani

AU - Mouritsen, Ole G.

AU - Goormaghtigh, Erik

AU - Kinnunen, Paavo K.J.

PY - 2006/11/14

Y1 - 2006/11/14

N2 - Recent publications described the formation of millimeter-length fibers by diverse lipid-binding proteins (e.g., histone H1, cytochrome c, indolicidin, and endostatin) when they are mixed with 80:20 phosphatidylcholine/ phosphatidylserine vesicles. Further, these fibers displayed amyloid characteristics when stained with Congo Red. In the study presented here, we found by FTIR the amide I absorption band to reveal significant variation in fibers formed by cytochrome c, with some consisting of cytochrome c in a nativelike conformation and some exhibiting strong amyloid (β-sheet) characteristics. Protein structure also varied from amyloid to nearly native within single fibers. Fibers were frequently blue or bluish and sometimes iridescent, likely due to interference of light in the fibers. The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing.

AB - Recent publications described the formation of millimeter-length fibers by diverse lipid-binding proteins (e.g., histone H1, cytochrome c, indolicidin, and endostatin) when they are mixed with 80:20 phosphatidylcholine/ phosphatidylserine vesicles. Further, these fibers displayed amyloid characteristics when stained with Congo Red. In the study presented here, we found by FTIR the amide I absorption band to reveal significant variation in fibers formed by cytochrome c, with some consisting of cytochrome c in a nativelike conformation and some exhibiting strong amyloid (β-sheet) characteristics. Protein structure also varied from amyloid to nearly native within single fibers. Fibers were frequently blue or bluish and sometimes iridescent, likely due to interference of light in the fibers. The amyloid-type amide I band was observed for blue fibers only. AFM shows that fibers consist of smaller 3-4 nm diameter fibers with 10 nm lateral spacing.

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VL - 45

SP - 13447

EP - 13453

JO - Biochemistry

JF - Biochemistry

IS - 45

ER -

Characteristics of fibers formed by cytochrome c and induced by anionic phospholipids

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