Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians

Mikkel Andreassen; Kaspar Nielsen; Ilan Raymond; Lars Østergaard Kristensen; Jens Faber; Mikkel Andreassen; Kaspar Rosengreen Nielsen; Ilan Raymond; Lars Østergaard Kristensen; Jens Faber

doi:10.3109/00365510903165477

Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians

Mikkel Andreassen, Kaspar Nielsen, Ilan Raymond, Lars Østergaard Kristensen, Jens Faber, Mikkel Andreassen, Kaspar Rosengreen Nielsen, Ilan Raymond, Lars Østergaard Kristensen, Jens Faber

10 Citations (Scopus)

Abstract

BACKGROUND AND OBJECTIVE: Measurements of Insulin-Like Growth Factor-I (IGF-I) play a pivotal role in the evaluation of the growth hormone-IGF-I axis. Due to assay variation IGF-I reference ranges are assay specific. We provide serum IGF-I reference ranges for adult men and women obtained by a commercially available assay. METHOD: IGF-I was measured by an enzyme-linked immunosorbent assay (R&D Systems). Assay precision was evaluated in low, medium and high IGF-I pools and in single samples from outpatients. The reference ranges were obtained in 724 healthy Caucasians, mean age 48 years (range 19-91). RESULTS: IGF-I was measured higher in serum compared to plasma samples (p<0.001, R2=0.96). To convert EDTA plasma values to serum values a factor of 1.22 was calculated. The intraassay coefficients of variation (CV) for the low: 56.4+/-2.7 ng/mL, medium: 179.7+/-5.9 ng/mL and high pool: 445.61+/-3.2 ng/mL (mean 1 SD) were 5, 3 and 3%. Interassays CVs for the low, medium and high pool varied between 7-10, 5-7, and 6-9%. Reproducibility between 4 different lots showed a intraclass CV of 0.99 (95%CI 0.98-0.96). Logarithmically transformed IGF-I levels were linearly associated with age with a 13% reduction in IGF-I per decade in females and 11% in males. CONCLUSION: We have provided IGF-I reference ranges obtained by an assay that showed variations and reproducibility that was considered of sufficient high quality for clinical and scientific use.

Original language	English
Journal	Scandinavian Journal of Clinical & Laboratory Investigation
Volume	69
Issue number	8
Pages (from-to)	880-5
Number of pages	6
ISSN	0036-5513
DOIs	https://doi.org/10.3109/00365510903165477 https://doi.org/10.3109/00365510903165477
Publication status	Published - 2009

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Andreassen, M., Nielsen, K., Raymond, I., Kristensen, L. Ø., Faber, J., Andreassen, M., Nielsen, K. R., Raymond, I., Kristensen, L. Ø., & Faber, J. (2009). Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians. Scandinavian Journal of Clinical & Laboratory Investigation, 69(8), 880-5. https://doi.org/10.3109/00365510903165477, https://doi.org/10.3109/00365510903165477

Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians. / Andreassen, Mikkel; Nielsen, Kaspar; Raymond, Ilan et al.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 69, No. 8, 2009, p. 880-5.

Research output: Contribution to journal › Journal article › Research › peer-review

Andreassen, M, Nielsen, K, Raymond, I, Kristensen, LØ, Faber, J, Andreassen, M, Nielsen, KR, Raymond, I, Kristensen, LØ & Faber, J 2009, 'Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians', Scandinavian Journal of Clinical & Laboratory Investigation, vol. 69, no. 8, pp. 880-5. https://doi.org/10.3109/00365510903165477, https://doi.org/10.3109/00365510903165477

Andreassen M, Nielsen K, Raymond I, Kristensen LØ, Faber J, Andreassen M et al. Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians. Scandinavian Journal of Clinical & Laboratory Investigation. 2009;69(8):880-5. doi: 10.3109/00365510903165477, http://dx.doi.org/10.3109/00365510903165477

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title = "Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians",

abstract = "BACKGROUND AND OBJECTIVE: Measurements of Insulin-Like Growth Factor-I (IGF-I) play a pivotal role in the evaluation of the growth hormone-IGF-I axis. Due to assay variation IGF-I reference ranges are assay specific. We provide serum IGF-I reference ranges for adult men and women obtained by a commercially available assay. METHOD: IGF-I was measured by an enzyme-linked immunosorbent assay (R&D Systems). Assay precision was evaluated in low, medium and high IGF-I pools and in single samples from outpatients. The reference ranges were obtained in 724 healthy Caucasians, mean age 48 years (range 19-91). RESULTS: IGF-I was measured higher in serum compared to plasma samples (p<0.001, R2=0.96). To convert EDTA plasma values to serum values a factor of 1.22 was calculated. The intraassay coefficients of variation (CV) for the low: 56.4+/-2.7 ng/mL, medium: 179.7+/-5.9 ng/mL and high pool: 445.61+/-3.2 ng/mL (mean 1 SD) were 5, 3 and 3%. Interassays CVs for the low, medium and high pool varied between 7-10, 5-7, and 6-9%. Reproducibility between 4 different lots showed a intraclass CV of 0.99 (95%CI 0.98-0.96). Logarithmically transformed IGF-I levels were linearly associated with age with a 13% reduction in IGF-I per decade in females and 11% in males. CONCLUSION: We have provided IGF-I reference ranges obtained by an assay that showed variations and reproducibility that was considered of sufficient high quality for clinical and scientific use.",

author = "Mikkel Andreassen and Kaspar Nielsen and Ilan Raymond and Kristensen, {Lars {\O}stergaard} and Jens Faber and Mikkel Andreassen and Nielsen, {Kaspar Rosengreen} and Ilan Raymond and Kristensen, {Lars {\O}stergaard} and Jens Faber",

note = "Keywords: Adult; Aged; Aged, 80 and over; Blood Specimen Collection; European Continental Ancestry Group; Female; Health; Humans; Immunoassay; Insulin-Like Growth Factor I; Male; Middle Aged; Reagent Kits, Diagnostic; Reference Standards; Reference Values; Sex Characteristics; Young Adult",

year = "2009",

doi = "10.3109/00365510903165477",

language = "English",

volume = "69",

pages = "880--5",

journal = "Scandinavian Journal of Clinical & Laboratory Investigation",

issn = "0036-5513",

publisher = "Taylor & Francis",

number = "8",

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TY - JOUR

T1 - Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians

AU - Andreassen, Mikkel

AU - Nielsen, Kaspar

AU - Raymond, Ilan

AU - Kristensen, Lars Østergaard

AU - Faber, Jens

AU - Andreassen, Mikkel

AU - Nielsen, Kaspar Rosengreen

AU - Raymond, Ilan

AU - Kristensen, Lars Østergaard

AU - Faber, Jens

N1 - Keywords: Adult; Aged; Aged, 80 and over; Blood Specimen Collection; European Continental Ancestry Group; Female; Health; Humans; Immunoassay; Insulin-Like Growth Factor I; Male; Middle Aged; Reagent Kits, Diagnostic; Reference Standards; Reference Values; Sex Characteristics; Young Adult

PY - 2009

Y1 - 2009

N2 - BACKGROUND AND OBJECTIVE: Measurements of Insulin-Like Growth Factor-I (IGF-I) play a pivotal role in the evaluation of the growth hormone-IGF-I axis. Due to assay variation IGF-I reference ranges are assay specific. We provide serum IGF-I reference ranges for adult men and women obtained by a commercially available assay. METHOD: IGF-I was measured by an enzyme-linked immunosorbent assay (R&D Systems). Assay precision was evaluated in low, medium and high IGF-I pools and in single samples from outpatients. The reference ranges were obtained in 724 healthy Caucasians, mean age 48 years (range 19-91). RESULTS: IGF-I was measured higher in serum compared to plasma samples (p<0.001, R2=0.96). To convert EDTA plasma values to serum values a factor of 1.22 was calculated. The intraassay coefficients of variation (CV) for the low: 56.4+/-2.7 ng/mL, medium: 179.7+/-5.9 ng/mL and high pool: 445.61+/-3.2 ng/mL (mean 1 SD) were 5, 3 and 3%. Interassays CVs for the low, medium and high pool varied between 7-10, 5-7, and 6-9%. Reproducibility between 4 different lots showed a intraclass CV of 0.99 (95%CI 0.98-0.96). Logarithmically transformed IGF-I levels were linearly associated with age with a 13% reduction in IGF-I per decade in females and 11% in males. CONCLUSION: We have provided IGF-I reference ranges obtained by an assay that showed variations and reproducibility that was considered of sufficient high quality for clinical and scientific use.

AB - BACKGROUND AND OBJECTIVE: Measurements of Insulin-Like Growth Factor-I (IGF-I) play a pivotal role in the evaluation of the growth hormone-IGF-I axis. Due to assay variation IGF-I reference ranges are assay specific. We provide serum IGF-I reference ranges for adult men and women obtained by a commercially available assay. METHOD: IGF-I was measured by an enzyme-linked immunosorbent assay (R&D Systems). Assay precision was evaluated in low, medium and high IGF-I pools and in single samples from outpatients. The reference ranges were obtained in 724 healthy Caucasians, mean age 48 years (range 19-91). RESULTS: IGF-I was measured higher in serum compared to plasma samples (p<0.001, R2=0.96). To convert EDTA plasma values to serum values a factor of 1.22 was calculated. The intraassay coefficients of variation (CV) for the low: 56.4+/-2.7 ng/mL, medium: 179.7+/-5.9 ng/mL and high pool: 445.61+/-3.2 ng/mL (mean 1 SD) were 5, 3 and 3%. Interassays CVs for the low, medium and high pool varied between 7-10, 5-7, and 6-9%. Reproducibility between 4 different lots showed a intraclass CV of 0.99 (95%CI 0.98-0.96). Logarithmically transformed IGF-I levels were linearly associated with age with a 13% reduction in IGF-I per decade in females and 11% in males. CONCLUSION: We have provided IGF-I reference ranges obtained by an assay that showed variations and reproducibility that was considered of sufficient high quality for clinical and scientific use.

U2 - 10.3109/00365510903165477

DO - 10.3109/00365510903165477

M3 - Journal article

C2 - 19675969

SN - 0036-5513

VL - 69

SP - 880

EP - 885

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

IS - 8

ER -

Characteristics and reference ranges of Insulin-Like Growth Factor-I measured with a commercially available immunoassay in 724 healthy adult Caucasians

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