Characterisation of chitin in the cuticle of a velvet worm (Onychophora)

Hartmut Greven; Murat Kaya; Idris Sargin; Talat Baran; Reinhardt Mobjerg Kristensen; Martin Vinther Sorensen

doi:10.3906/zoo-1903-37

Characterisation of chitin in the cuticle of a velvet worm (Onychophora)

Hartmut Greven, Murat Kaya, Idris Sargin, Talat Baran, Reinhardt Mobjerg Kristensen, Martin Vinther Sorensen

1 Citation (Scopus)

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Abstract

We characterize the trunk cuticle of velvet worms of the Peripatoides novaezealandiae-group (Onychophora) using SEM, TEM, Fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA). TEM and SEM revealed a relatively uniform organization of the delicate cuticle that is covered by numerous bristled and nonbristled papillae with ribbed scales arranged in transverse rows. The cuticle consists of a very thin multilayered epicuticle of varying appearance followed by the largely fibrous procuticle. The irregularly arranged nanofibres of isolated cuticular chitin seen by SEM are considered as bundles of chitin fibres. FT-IR and TGA showed that the chitin is of the α-type. This confirms and broadens the single previous study in which the presence of α-chitin in a velvet worm was demonstrated with a single analysis (X-ray diffraction).

Original language	English
Journal	Turkish Journal of Zoology
Volume	43
Issue number	5
Pages (from-to)	416-424
ISSN	1300-0179
DOIs	https://doi.org/10.3906/zoo-1903-37
Publication status	Published - 2019

Keywords

Chitin
Fourier transform infrared spectroscopy
thermogravimetric analysis
Peripatoides
cuticle
Ecdysozoa

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10.3906/zoo-1903-37

zoo-43-5-2-1903-37Final published version, 3.07 MBLicence: CC BY

https://doi.org/10.3906/zoo-1903-37

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title = "Characterisation of chitin in the cuticle of a velvet worm (Onychophora)",

abstract = "We characterize the trunk cuticle of velvet worms of the Peripatoides novaezealandiae-group (Onychophora) using SEM, TEM, Fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA). TEM and SEM revealed a relatively uniform organization of the delicate cuticle that is covered by numerous bristled and nonbristled papillae with ribbed scales arranged in transverse rows. The cuticle consists of a very thin multilayered epicuticle of varying appearance followed by the largely fibrous procuticle. The irregularly arranged nanofibres of isolated cuticular chitin seen by SEM are considered as bundles of chitin fibres. FT-IR and TGA showed that the chitin is of the α-type. This confirms and broadens the single previous study in which the presence of α-chitin in a velvet worm was demonstrated with a single analysis (X-ray diffraction).",

keywords = "Chitin, Fourier transform infrared spectroscopy, thermogravimetric analysis, Peripatoides, cuticle, Ecdysozoa",

author = "Hartmut Greven and Murat Kaya and Idris Sargin and Talat Baran and Kristensen, {Reinhardt Mobjerg} and Sorensen, {Martin Vinther}",

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language = "English",

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pages = "416--424",

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TY - JOUR

T1 - Characterisation of chitin in the cuticle of a velvet worm (Onychophora)

AU - Greven, Hartmut

AU - Kaya, Murat

AU - Sargin, Idris

AU - Baran, Talat

AU - Kristensen, Reinhardt Mobjerg

AU - Sorensen, Martin Vinther

PY - 2019

Y1 - 2019

N2 - We characterize the trunk cuticle of velvet worms of the Peripatoides novaezealandiae-group (Onychophora) using SEM, TEM, Fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA). TEM and SEM revealed a relatively uniform organization of the delicate cuticle that is covered by numerous bristled and nonbristled papillae with ribbed scales arranged in transverse rows. The cuticle consists of a very thin multilayered epicuticle of varying appearance followed by the largely fibrous procuticle. The irregularly arranged nanofibres of isolated cuticular chitin seen by SEM are considered as bundles of chitin fibres. FT-IR and TGA showed that the chitin is of the α-type. This confirms and broadens the single previous study in which the presence of α-chitin in a velvet worm was demonstrated with a single analysis (X-ray diffraction).

AB - We characterize the trunk cuticle of velvet worms of the Peripatoides novaezealandiae-group (Onychophora) using SEM, TEM, Fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA). TEM and SEM revealed a relatively uniform organization of the delicate cuticle that is covered by numerous bristled and nonbristled papillae with ribbed scales arranged in transverse rows. The cuticle consists of a very thin multilayered epicuticle of varying appearance followed by the largely fibrous procuticle. The irregularly arranged nanofibres of isolated cuticular chitin seen by SEM are considered as bundles of chitin fibres. FT-IR and TGA showed that the chitin is of the α-type. This confirms and broadens the single previous study in which the presence of α-chitin in a velvet worm was demonstrated with a single analysis (X-ray diffraction).

KW - Chitin

KW - Fourier transform infrared spectroscopy

KW - thermogravimetric analysis

KW - Peripatoides

KW - cuticle

KW - Ecdysozoa

U2 - 10.3906/zoo-1903-37

DO - 10.3906/zoo-1903-37

M3 - Journal article

SN - 1300-0179

VL - 43

SP - 416

EP - 424

JO - Turkish Journal of Zoology

JF - Turkish Journal of Zoology

IS - 5

ER -

Characterisation of chitin in the cuticle of a velvet worm (Onychophora)

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Keywords

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