TY - JOUR
T1 - Changes in S100 Proteins Identified in Healthy Skin following Electrical Stimulation
T2 - Relevance for Wound Healing
AU - Lallyett, Chloe
AU - Yeung, Ching-Yan Chloé
AU - Nielson, Rie Harboe
AU - Zeef, Leo A H
AU - Chapman-Jones, David
AU - Kjaer, Michael
AU - Kadler, Karl E
PY - 2018/7/1
Y1 - 2018/7/1
N2 - OBJECTIVE: Targeted electrical energy applied to wounds has been shown to improve wound-healing rates. However, the mechanisms are poorly understood. The aim of this study was to identify genes that are responsive to electrical stimulation (ES) in healthy subjects with undamaged skin.METHODS: To achieve this objective, study authors used a small, noninvasive ES medical device to deliver a continuous, specific, set sequence of electrical energy impulses over a 48-hour period to the skin of healthy volunteers and compared resultant gene expression by microarray analysis.MAIN RESULTS: Application of this specific ES resulted in differential expression of 105 genes, the majority of which were down-regulated. Postmicroarray analyses revealed there was commonality with a small number of genes that have previously been shown to be up-regulated in skin wounds, including venous leg ulcers.CONCLUSIONS: The specific sequence of ES applied continuously for 48 hours to the skin of healthy patients has the effect of modifying expression in a number of identified genes. The identification of the differential expression in this subset of genes in healthy subjects provides new potential lines of scientific inquiry for identifying similar responses in subjects with slow or poorly healing wounds.
AB - OBJECTIVE: Targeted electrical energy applied to wounds has been shown to improve wound-healing rates. However, the mechanisms are poorly understood. The aim of this study was to identify genes that are responsive to electrical stimulation (ES) in healthy subjects with undamaged skin.METHODS: To achieve this objective, study authors used a small, noninvasive ES medical device to deliver a continuous, specific, set sequence of electrical energy impulses over a 48-hour period to the skin of healthy volunteers and compared resultant gene expression by microarray analysis.MAIN RESULTS: Application of this specific ES resulted in differential expression of 105 genes, the majority of which were down-regulated. Postmicroarray analyses revealed there was commonality with a small number of genes that have previously been shown to be up-regulated in skin wounds, including venous leg ulcers.CONCLUSIONS: The specific sequence of ES applied continuously for 48 hours to the skin of healthy patients has the effect of modifying expression in a number of identified genes. The identification of the differential expression in this subset of genes in healthy subjects provides new potential lines of scientific inquiry for identifying similar responses in subjects with slow or poorly healing wounds.
KW - Electric Stimulation/methods
KW - Healthy Volunteers
KW - Humans
KW - S100 Proteins/physiology
KW - Skin/injuries
KW - Wound Healing/physiology
U2 - 10.1097/01.ASW.0000533722.06780.03
DO - 10.1097/01.ASW.0000533722.06780.03
M3 - Journal article
C2 - 29923902
SN - 1527-7941
VL - 31
SP - 322
EP - 327
JO - Advances in Skin and Wound Care
JF - Advances in Skin and Wound Care
IS - 7
ER -