Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep

Wenche Jørgensen; Christiane Marie Bourgin Gam; Jesper Løvind Andersen; Peter Schjerling; Morten Scheibye-Knudsen; Ole Hartvig Mortensen; Niels Grunnet; Mette Benedicte Olaf Nielsen; Bjørn Quistorff

doi:10.1152/ajpendo.00505.2009

Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep

Wenche Jørgensen, Christiane Marie Bourgin Gam, Jesper Løvind Andersen, Peter Schjerling, Morten Scheibye-Knudsen, Ole Hartvig Mortensen, Niels Grunnet, Mette Benedicte Olaf Nielsen, Bjørn Quistorff

20 Citations (Scopus)

Abstract

In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an approximately 30% increase (P < 0.05) of mitochondrial VO(2max) and an approximately 50% increase (P < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1alpha mRNA levels (P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower VO(2max) (approximately 40%, P < 0.05), a lower state 4 respiration (approximately 40%, P < 0.05), and lower RCR ( approximately 15%, P < 0.05). Both PGC-1alpha and UCP3 mRNA levels were increased (P < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced VO(2max) in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and VO(2max) increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood.

Original language	English
Journal	American Journal of Physiology: Endocrinology and Metabolism
Volume	297
Issue number	6
Pages (from-to)	E1349-1357
Number of pages	9
ISSN	0193-1849
DOIs	https://doi.org/10.1152/ajpendo.00505.2009
Publication status	Published - 2009

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10.1152/ajpendo.00505.2009

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@article{22e87070334611df8ed1000ea68e967b,

title = "Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep",

abstract = "In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an approximately 30% increase (P < 0.05) of mitochondrial VO(2max) and an approximately 50% increase (P < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1alpha mRNA levels (P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower VO(2max) (approximately 40%, P < 0.05), a lower state 4 respiration (approximately 40%, P < 0.05), and lower RCR ( approximately 15%, P < 0.05). Both PGC-1alpha and UCP3 mRNA levels were increased (P < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced VO(2max) in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and VO(2max) increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood.",

author = "Wenche J{\o}rgensen and Gam, {Christiane Marie Bourgin} and Andersen, {Jesper L{\o}vind} and Peter Schjerling and Morten Scheibye-Knudsen and Mortensen, {Ole Hartvig} and Niels Grunnet and Nielsen, {Mette Benedicte Olaf} and Bj{\o}rn Quistorff",

note = "Keywords: Animals; Animals, Newborn; Biopsy; DNA, Mitochondrial; Female; Fetal Development; Malnutrition; Maternal Nutritional Physiological Phenomena; Mitochondria, Muscle; Muscle Fibers, Skeletal; Muscle, Skeletal; Oxygen Consumption; PPAR delta; Pregnancy; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Sheep; Transcription Factors; Uncoupling Agents",

year = "2009",

doi = "10.1152/ajpendo.00505.2009",

language = "English",

volume = "297",

pages = "E1349--1357",

journal = "American Journal of Physiology - Endocrinology and Metabolism",

issn = "0193-1849",

publisher = "American Physiological Society",

number = "6",

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TY - JOUR

T1 - Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep

AU - Jørgensen, Wenche

AU - Gam, Christiane Marie Bourgin

AU - Andersen, Jesper Løvind

AU - Schjerling, Peter

AU - Scheibye-Knudsen, Morten

AU - Mortensen, Ole Hartvig

AU - Grunnet, Niels

AU - Nielsen, Mette Benedicte Olaf

AU - Quistorff, Bjørn

N1 - Keywords: Animals; Animals, Newborn; Biopsy; DNA, Mitochondrial; Female; Fetal Development; Malnutrition; Maternal Nutritional Physiological Phenomena; Mitochondria, Muscle; Muscle Fibers, Skeletal; Muscle, Skeletal; Oxygen Consumption; PPAR delta; Pregnancy; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Sheep; Transcription Factors; Uncoupling Agents

PY - 2009

Y1 - 2009

N2 - In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an approximately 30% increase (P < 0.05) of mitochondrial VO(2max) and an approximately 50% increase (P < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1alpha mRNA levels (P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower VO(2max) (approximately 40%, P < 0.05), a lower state 4 respiration (approximately 40%, P < 0.05), and lower RCR ( approximately 15%, P < 0.05). Both PGC-1alpha and UCP3 mRNA levels were increased (P < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced VO(2max) in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and VO(2max) increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood.

AB - In a sheep model, we investigated diet effects on skeletal muscle mitochondria to look for fetal programming. During pregnancy, ewes were fed normally (N) or were 50% food restricted (L) during the last trimester, and lambs born to these ewes received a normal (N) or a high-fat diet (H) for the first 6 mo of life. We examined mitochondrial function in permeabilized muscle fibers from the lambs at 6 mo of age (adolescence) and after 24 mo of age (adulthood). The postpartum H diet for the lambs induced an approximately 30% increase (P < 0.05) of mitochondrial VO(2max) and an approximately 50% increase (P < 0.05) of the respiratory coupling ratio (RCR) combined with lower levels of UCP3 and PGC-1alpha mRNA levels (P < 0.05). These effects proved to be reversible by a normal diet from 6 to 24 mo of age. However, at 24 mo, a long-term effect of the maternal gestational diet restriction (fetal programming) became evident as a lower VO(2max) (approximately 40%, P < 0.05), a lower state 4 respiration (approximately 40%, P < 0.05), and lower RCR ( approximately 15%, P < 0.05). Both PGC-1alpha and UCP3 mRNA levels were increased (P < 0.05). Two analyzed muscles were affected differently, and muscle rich in type I fibers was more susceptible to fetal programming. We conclude that fetal programming, seen as a reduced VO(2max) in adulthood, results from gestational undernutrition. Postnatal high-fat diet results in a pronounced RCR and VO(2max) increase in adolescence. However, these effects are reversible by diet correction and are not maintained in adulthood.

U2 - 10.1152/ajpendo.00505.2009

DO - 10.1152/ajpendo.00505.2009

M3 - Journal article

C2 - 19826104

SN - 0193-1849

VL - 297

SP - E1349-1357

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

IS - 6

ER -

Changed mitochondrial function by pre- and/or postpartum diet alterations in sheep

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