CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium

Maren Mönnich; Louise Borgeskov; Loretta Breslin; Lis Jakobsen; Michaela Rogowski; Canan Doganli; Jacob Morville Schrøder; Johanne Bay Mogensen; Louise Blinkenkjær; Lea M. Harder; Emma Lundberg; Stefan Geimer; Søren Tvorup Christensen; Jens S. Andersen; Lars Allan Larsen; Lotte Bang Pedersen

doi:10.1016/j.celrep.2018.02.043

CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium

Maren Mönnich, Louise Borgeskov, Loretta Breslin, Lis Jakobsen, Michaela Rogowski, Canan Doganli, Jacob Morville Schrøder, Johanne Bay Mogensen, Louise Blinkenkjær, Lea M. Harder, Emma Lundberg, Stefan Geimer, Søren Tvorup Christensen, Jens S. Andersen, Lars Allan Larsen, Lotte Bang Pedersen

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Abstract

The centrosome is the main microtubule-organizing center in animal cells and comprises a mother and daughter centriole surrounded by pericentriolar material. During formation of primary cilia, the mother centriole transforms into a basal body that templates the ciliary axoneme. Ciliogenesis depends on mother centriole-specific distal appendages, whereas the role of subdistal appendages in ciliary function is unclear. Here, we identify CEP128 as a centriole subdistal appendage protein required for regulating ciliary signaling. Loss of CEP128 did not grossly affect centrosomal or ciliary structure but caused impaired transforming growth factor-β/bone morphogenetic protein (TGF-β/BMP) signaling in zebrafish and at the primary cilium in cultured mammalian cells. This phenotype is likely the result of defective vesicle trafficking at the cilium as ciliary localization of RAB11 was impaired upon loss of CEP128, and quantitative phosphoproteomics revealed that CEP128 loss affects TGF-β1-induced phosphorylation of multiple proteins that regulate cilium-associated vesicle trafficking. Mönnich et al. show that CEP128 localizes to the subdistal appendages of the mother centriole and basal body of the primary cilium. CEP128 regulates vesicular trafficking and targeting of RAB11 to the primary cilium. CEP128 loss leads to impaired TGF-β/BMP signaling, which, in zebrafish, is associated with defective organ development.

Original language	English
Journal	Cell Reports
Volume	22
Issue number	10
Pages (from-to)	2584-2592
Number of pages	9
ISSN	2211-1247
DOIs	https://doi.org/10.1016/j.celrep.2018.02.043
Publication status	Published - 6 Mar 2018

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10.1016/j.celrep.2018.02.043Licence: CC BY-NC-ND

CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and RegulatesFinal published version, 2.66 MBLicence: CC BY-NC-ND

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Mönnich, M., Borgeskov, L., Breslin, L., Jakobsen, L., Rogowski, M., Doganli, C., Schrøder, J. M., Mogensen, J. B., Blinkenkjær, L., Harder, L. M., Lundberg, E., Geimer, S., Christensen, S. T., Andersen, J. S., Larsen, L. A., & Pedersen, L. B. (2018). CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium. Cell Reports, 22(10), 2584-2592. https://doi.org/10.1016/j.celrep.2018.02.043

Mönnich, M, Borgeskov, L, Breslin, L, Jakobsen, L, Rogowski, M, Doganli, C, Schrøder, JM, Mogensen, JB, Blinkenkjær, L, Harder, LM, Lundberg, E, Geimer, S, Christensen, ST, Andersen, JS, Larsen, LA & Pedersen, LB 2018, 'CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium', Cell Reports, vol. 22, no. 10, pp. 2584-2592. https://doi.org/10.1016/j.celrep.2018.02.043

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title = "CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium",

abstract = "The centrosome is the main microtubule-organizing center in animal cells and comprises a mother and daughter centriole surrounded by pericentriolar material. During formation of primary cilia, the mother centriole transforms into a basal body that templates the ciliary axoneme. Ciliogenesis depends on mother centriole-specific distal appendages, whereas the role of subdistal appendages in ciliary function is unclear. Here, we identify CEP128 as a centriole subdistal appendage protein required for regulating ciliary signaling. Loss of CEP128 did not grossly affect centrosomal or ciliary structure but caused impaired transforming growth factor-β/bone morphogenetic protein (TGF-β/BMP) signaling in zebrafish and at the primary cilium in cultured mammalian cells. This phenotype is likely the result of defective vesicle trafficking at the cilium as ciliary localization of RAB11 was impaired upon loss of CEP128, and quantitative phosphoproteomics revealed that CEP128 loss affects TGF-β1-induced phosphorylation of multiple proteins that regulate cilium-associated vesicle trafficking. M{\"o}nnich et al. show that CEP128 localizes to the subdistal appendages of the mother centriole and basal body of the primary cilium. CEP128 regulates vesicular trafficking and targeting of RAB11 to the primary cilium. CEP128 loss leads to impaired TGF-β/BMP signaling, which, in zebrafish, is associated with defective organ development.",

author = "Maren M{\"o}nnich and Louise Borgeskov and Loretta Breslin and Lis Jakobsen and Michaela Rogowski and Canan Doganli and Schr{\o}der, {Jacob Morville} and Mogensen, {Johanne Bay} and Louise Blinkenkj{\ae}r and Harder, {Lea M.} and Emma Lundberg and Stefan Geimer and Christensen, {S{\o}ren Tvorup} and Andersen, {Jens S.} and Larsen, {Lars Allan} and Pedersen, {Lotte Bang}",

year = "2018",

month = mar,

day = "6",

doi = "10.1016/j.celrep.2018.02.043",

language = "English",

volume = "22",

pages = "2584--2592",

journal = "Cell Reports",

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T1 - CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium

AU - Mönnich, Maren

AU - Borgeskov, Louise

AU - Breslin, Loretta

AU - Jakobsen, Lis

AU - Rogowski, Michaela

AU - Doganli, Canan

AU - Schrøder, Jacob Morville

AU - Mogensen, Johanne Bay

AU - Blinkenkjær, Louise

AU - Harder, Lea M.

AU - Lundberg, Emma

AU - Geimer, Stefan

AU - Christensen, Søren Tvorup

AU - Andersen, Jens S.

AU - Larsen, Lars Allan

AU - Pedersen, Lotte Bang

PY - 2018/3/6

Y1 - 2018/3/6

N2 - The centrosome is the main microtubule-organizing center in animal cells and comprises a mother and daughter centriole surrounded by pericentriolar material. During formation of primary cilia, the mother centriole transforms into a basal body that templates the ciliary axoneme. Ciliogenesis depends on mother centriole-specific distal appendages, whereas the role of subdistal appendages in ciliary function is unclear. Here, we identify CEP128 as a centriole subdistal appendage protein required for regulating ciliary signaling. Loss of CEP128 did not grossly affect centrosomal or ciliary structure but caused impaired transforming growth factor-β/bone morphogenetic protein (TGF-β/BMP) signaling in zebrafish and at the primary cilium in cultured mammalian cells. This phenotype is likely the result of defective vesicle trafficking at the cilium as ciliary localization of RAB11 was impaired upon loss of CEP128, and quantitative phosphoproteomics revealed that CEP128 loss affects TGF-β1-induced phosphorylation of multiple proteins that regulate cilium-associated vesicle trafficking. Mönnich et al. show that CEP128 localizes to the subdistal appendages of the mother centriole and basal body of the primary cilium. CEP128 regulates vesicular trafficking and targeting of RAB11 to the primary cilium. CEP128 loss leads to impaired TGF-β/BMP signaling, which, in zebrafish, is associated with defective organ development.

AB - The centrosome is the main microtubule-organizing center in animal cells and comprises a mother and daughter centriole surrounded by pericentriolar material. During formation of primary cilia, the mother centriole transforms into a basal body that templates the ciliary axoneme. Ciliogenesis depends on mother centriole-specific distal appendages, whereas the role of subdistal appendages in ciliary function is unclear. Here, we identify CEP128 as a centriole subdistal appendage protein required for regulating ciliary signaling. Loss of CEP128 did not grossly affect centrosomal or ciliary structure but caused impaired transforming growth factor-β/bone morphogenetic protein (TGF-β/BMP) signaling in zebrafish and at the primary cilium in cultured mammalian cells. This phenotype is likely the result of defective vesicle trafficking at the cilium as ciliary localization of RAB11 was impaired upon loss of CEP128, and quantitative phosphoproteomics revealed that CEP128 loss affects TGF-β1-induced phosphorylation of multiple proteins that regulate cilium-associated vesicle trafficking. Mönnich et al. show that CEP128 localizes to the subdistal appendages of the mother centriole and basal body of the primary cilium. CEP128 regulates vesicular trafficking and targeting of RAB11 to the primary cilium. CEP128 loss leads to impaired TGF-β/BMP signaling, which, in zebrafish, is associated with defective organ development.

U2 - 10.1016/j.celrep.2018.02.043

DO - 10.1016/j.celrep.2018.02.043

M3 - Journal article

C2 - 29514088

SN - 2211-1247

VL - 22

SP - 2584

EP - 2592

JO - Cell Reports

JF - Cell Reports

IS - 10

ER -

CEP128 Localizes to the Subdistal Appendages of the Mother Centriole and Regulates TGF-β/BMP Signaling at the Primary Cilium

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