Cellulase Inhibition by High Concentrations of Monosaccharides

Chia-Wen Hsieh; David Cannella; Henning Jørgensen; Claus Felby; Lisbeth Garbrecht Thygesen

doi:10.1021/jf5012962

Cellulase Inhibition by High Concentrations of Monosaccharides

Chia-Wen Hsieh, David Cannella, Henning Jørgensen, Claus Felby, Lisbeth Garbrecht Thygesen

Forest, Nature and Biomass

86 Citations (Scopus)

Abstract

Biological degradation of biomass on an industrial scale culminates in high concentrations of end products. It is known that the accumulation of glucose and cellobiose, end products of hydrolysis, inhibit cellulases and decrease glucose yields. Aside from these end products, however, other monosaccharides such as mannose and galactose (stereoisomers of glucose) decrease glucose yields as well. NMR relaxometry measurements showed direct correlations between the initial T2 of the liquid phase in which hydrolysis takes place and the total glucose production during cellulose hydrolysis, indicating that low free water availability contributes to cellulase inhibition. Of the hydrolytic enzymes involved, those acting on the cellulose substrate, that is, exo- and endoglucanases, were the most inhibited. The β-glucosidases were shown to be less sensitive to high monosaccharide concentrations except glucose. Protein adsorption studies showed that this inhibition effect was most likely due to catalytic, and not binding, inhibition of the cellulases.

Original language	English
Journal	Journal of Agricultural and Food Chemistry
Volume	62
Issue number	17
Pages (from-to)	3800-3805
Number of pages	6
ISSN	0021-8561
DOIs	https://doi.org/10.1021/jf5012962
Publication status	Published - 30 Apr 2014

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title = "Cellulase Inhibition by High Concentrations of Monosaccharides",

abstract = "Biological degradation of biomass on an industrial scale culminates in high concentrations of end products. It is known that the accumulation of glucose and cellobiose, end products of hydrolysis, inhibit cellulases and decrease glucose yields. Aside from these end products, however, other monosaccharides such as mannose and galactose (stereoisomers of glucose) decrease glucose yields as well. NMR relaxometry measurements showed direct correlations between the initial T2 of the liquid phase in which hydrolysis takes place and the total glucose production during cellulose hydrolysis, indicating that low free water availability contributes to cellulase inhibition. Of the hydrolytic enzymes involved, those acting on the cellulose substrate, that is, exo- and endoglucanases, were the most inhibited. The β-glucosidases were shown to be less sensitive to high monosaccharide concentrations except glucose. Protein adsorption studies showed that this inhibition effect was most likely due to catalytic, and not binding, inhibition of the cellulases.",

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T1 - Cellulase Inhibition by High Concentrations of Monosaccharides

AU - Hsieh, Chia-Wen

AU - Cannella, David

AU - Jørgensen, Henning

AU - Felby, Claus

AU - Thygesen, Lisbeth Garbrecht

PY - 2014/4/30

Y1 - 2014/4/30

N2 - Biological degradation of biomass on an industrial scale culminates in high concentrations of end products. It is known that the accumulation of glucose and cellobiose, end products of hydrolysis, inhibit cellulases and decrease glucose yields. Aside from these end products, however, other monosaccharides such as mannose and galactose (stereoisomers of glucose) decrease glucose yields as well. NMR relaxometry measurements showed direct correlations between the initial T2 of the liquid phase in which hydrolysis takes place and the total glucose production during cellulose hydrolysis, indicating that low free water availability contributes to cellulase inhibition. Of the hydrolytic enzymes involved, those acting on the cellulose substrate, that is, exo- and endoglucanases, were the most inhibited. The β-glucosidases were shown to be less sensitive to high monosaccharide concentrations except glucose. Protein adsorption studies showed that this inhibition effect was most likely due to catalytic, and not binding, inhibition of the cellulases.

AB - Biological degradation of biomass on an industrial scale culminates in high concentrations of end products. It is known that the accumulation of glucose and cellobiose, end products of hydrolysis, inhibit cellulases and decrease glucose yields. Aside from these end products, however, other monosaccharides such as mannose and galactose (stereoisomers of glucose) decrease glucose yields as well. NMR relaxometry measurements showed direct correlations between the initial T2 of the liquid phase in which hydrolysis takes place and the total glucose production during cellulose hydrolysis, indicating that low free water availability contributes to cellulase inhibition. Of the hydrolytic enzymes involved, those acting on the cellulose substrate, that is, exo- and endoglucanases, were the most inhibited. The β-glucosidases were shown to be less sensitive to high monosaccharide concentrations except glucose. Protein adsorption studies showed that this inhibition effect was most likely due to catalytic, and not binding, inhibition of the cellulases.

U2 - 10.1021/jf5012962

DO - 10.1021/jf5012962

M3 - Journal article

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JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

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Cellulase Inhibition by High Concentrations of Monosaccharides

Abstract

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