Cellular uptake of metallated cobalamins

Mai Thanh Quynh Tran; Stefan Stürup; Ian Henry Lambert; Bente Gammelgaard; Evelyne Furger; Roger Alberto

doi:10.1039/c5mt00272a

Cellular uptake of metallated cobalamins

Mai Thanh Quynh Tran, Stefan Stürup, Ian Henry Lambert, Bente Gammelgaard, Evelyne Furger, Roger Alberto

Cell Biology and Physiology

11 Citations (Scopus)

85 Downloads (Pure)

Abstract

Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-) and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[Pt(II)Cl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2](+), [{Co}-CN-{cis-PtCl(NH3)2}](+), [{Re}-{Co}-CN-{cis-PtCl(NH3)2}](+), and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}](2+) (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12.

Original language	English
Journal	Metallomics
Volume	8
Issue number	3
Pages (from-to)	298-304
Number of pages	7
ISSN	1756-5901
DOIs	https://doi.org/10.1039/c5mt00272a
Publication status	Published - Mar 2016

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@article{cea21e068de14b96872cd15fca814133,

title = "Cellular uptake of metallated cobalamins",

abstract = "Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-) and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[Pt(II)Cl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2](+), [{Co}-CN-{cis-PtCl(NH3)2}](+), [{Re}-{Co}-CN-{cis-PtCl(NH3)2}](+), and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}](2+) (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12.",

author = "Tran, {Mai Thanh Quynh} and Stefan St{\"u}rup and Lambert, {Ian Henry} and Bente Gammelgaard and Evelyne Furger and Roger Alberto",

year = "2016",

month = mar,

doi = "10.1039/c5mt00272a",

language = "English",

volume = "8",

pages = "298--304",

journal = "Metallomics",

issn = "1756-5901",

publisher = "Royal Society of Chemistry",

number = "3",

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TY - JOUR

T1 - Cellular uptake of metallated cobalamins

AU - Tran, Mai Thanh Quynh

AU - Stürup, Stefan

AU - Lambert, Ian Henry

AU - Gammelgaard, Bente

AU - Furger, Evelyne

AU - Alberto, Roger

PY - 2016/3

Y1 - 2016/3

N2 - Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-) and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[Pt(II)Cl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2](+), [{Co}-CN-{cis-PtCl(NH3)2}](+), [{Re}-{Co}-CN-{cis-PtCl(NH3)2}](+), and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}](2+) (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12.

AB - Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-) and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[Pt(II)Cl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2](+), [{Co}-CN-{cis-PtCl(NH3)2}](+), [{Re}-{Co}-CN-{cis-PtCl(NH3)2}](+), and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}](2+) (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12.

U2 - 10.1039/c5mt00272a

DO - 10.1039/c5mt00272a

M3 - Journal article

C2 - 26739575

SN - 1756-5901

VL - 8

SP - 298

EP - 304

JO - Metallomics

JF - Metallomics

IS - 3

ER -

Cellular uptake of metallated cobalamins

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