Cell cycle regulation of the cyclin A gene promoter is mediated by a variant E2F site.

TY - JOUR

T1 - Cell cycle regulation of the cyclin A gene promoter is mediated by a variant E2F site.

AU - Schulze, A

AU - Zerfass, K

AU - Spitkovsky, D

AU - Middendorp, S

AU - Bergès, J

AU - Helin, K

AU - Jansen-Dürr, P

AU - Henglein, B

N1 - Keywords: 3T3 Cells; Animals; Base Sequence; Carrier Proteins; Cell Cycle; Cell Cycle Proteins; Cyclin D1; Cyclin-Dependent Kinase 4; Cyclin-Dependent Kinase Inhibitor p16; Cyclin-Dependent Kinases; Cyclins; DNA-Binding Proteins; E2F Transcription Factors; Gene Expression; Mice; Molecular Sequence Data; Oligodeoxyribonucleotides; Oncogene Proteins; Promoter Regions (Genetics); Proto-Oncogene Proteins; RNA, Messenger; Trans-Activation (Genetics); Transcription Factor DP1; Transcription Factors; Transcription, Genetic

PY - 1995

Y1 - 1995

N2 - Cyclin A is involved in the control of S phase and mitosis in mammalian cells. Expression of the cyclin A gene in nontransformed cells is characterized by repression of its promoter during the G1 phase of the cell cycle and its induction at S-phase entry. We show that this mode of regulation is mediated by the transcription factor E2F, which binds to a specific site in the cyclin A promoter. It differs from the prototype E2F site in nucleotide sequence and protein binding; it is bound by E2F complexes containing cyclin E and p107 but not pRB. Ectopic expression of cyclin D1 triggers premature activation of the cyclin A promoter by E2F, and this effect is blocked by the tumor suppressor protein p16INK4.

AB - Cyclin A is involved in the control of S phase and mitosis in mammalian cells. Expression of the cyclin A gene in nontransformed cells is characterized by repression of its promoter during the G1 phase of the cell cycle and its induction at S-phase entry. We show that this mode of regulation is mediated by the transcription factor E2F, which binds to a specific site in the cyclin A promoter. It differs from the prototype E2F site in nucleotide sequence and protein binding; it is bound by E2F complexes containing cyclin E and p107 but not pRB. Ectopic expression of cyclin D1 triggers premature activation of the cyclin A promoter by E2F, and this effect is blocked by the tumor suppressor protein p16INK4.

M3 - Journal article

C2 - 7479977

SN - 0027-8424

VL - 92

SP - 11264

EP - 11268

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -