Carry-over of host nutrients during sampling enhances undesired growth of Staphylococcus aureus in liquid Amies transport medium

Md Zohorul Islam; Jesper Larsen; Robert Skov; Øystein Angen

doi:10.1016/j.diagmicrobio.2018.07.009

Carry-over of host nutrients during sampling enhances undesired growth of Staphylococcus aureus in liquid Amies transport medium

Md Zohorul Islam^*, Jesper Larsen, Robert Skov, Øystein Angen

^*Corresponding author for this work

1 Citation (Scopus)

Abstract

Optimal transportation of bacteria is important for accurate clinical interpretation, quantitative assays, and microbiome studies. A transport medium should ideally keep the bacteria alive without supporting growth or altering the relative proportions of the constituent species. We investigated the effect of nasal mucus and mucin on the growth and survival of two Staphylococcus aureus strains in liquid Amies transport medium at room temperature and 4 °C for 14 days. The study showed that the presence of nasal mucus in microbiological samples stimulated undesired S. aureus growth at room temperature in a dose-dependent manner. These findings underscore that microbiological samples from humans and animals should be stored at 4 °C until analysis to avoid undesired S. aureus growth.

Original language	English
Journal	Diagnostic Microbiology and Infectious Disease
Volume	93
Issue number	1
Pages (from-to)	5-8
ISSN	0732-8893
DOIs	https://doi.org/10.1016/j.diagmicrobio.2018.07.009
Publication status	Published - Jan 2019

Keywords

Bacterial growth
Clinical specimens
S. aureus
Transport medium

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10.1016/j.diagmicrobio.2018.07.009Licence: CC BY-NC-ND

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title = "Carry-over of host nutrients during sampling enhances undesired growth of Staphylococcus aureus in liquid Amies transport medium",

abstract = "Optimal transportation of bacteria is important for accurate clinical interpretation, quantitative assays, and microbiome studies. A transport medium should ideally keep the bacteria alive without supporting growth or altering the relative proportions of the constituent species. We investigated the effect of nasal mucus and mucin on the growth and survival of two Staphylococcus aureus strains in liquid Amies transport medium at room temperature and 4 °C for 14 days. The study showed that the presence of nasal mucus in microbiological samples stimulated undesired S. aureus growth at room temperature in a dose-dependent manner. These findings underscore that microbiological samples from humans and animals should be stored at 4 °C until analysis to avoid undesired S. aureus growth.",

keywords = "Bacterial growth, Clinical specimens, S. aureus, Transport medium",

author = "Islam, {Md Zohorul} and Jesper Larsen and Robert Skov and {\O}ystein Angen",

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doi = "10.1016/j.diagmicrobio.2018.07.009",

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T1 - Carry-over of host nutrients during sampling enhances undesired growth of Staphylococcus aureus in liquid Amies transport medium

AU - Islam, Md Zohorul

AU - Larsen, Jesper

AU - Skov, Robert

AU - Angen, Øystein

PY - 2019/1

Y1 - 2019/1

N2 - Optimal transportation of bacteria is important for accurate clinical interpretation, quantitative assays, and microbiome studies. A transport medium should ideally keep the bacteria alive without supporting growth or altering the relative proportions of the constituent species. We investigated the effect of nasal mucus and mucin on the growth and survival of two Staphylococcus aureus strains in liquid Amies transport medium at room temperature and 4 °C for 14 days. The study showed that the presence of nasal mucus in microbiological samples stimulated undesired S. aureus growth at room temperature in a dose-dependent manner. These findings underscore that microbiological samples from humans and animals should be stored at 4 °C until analysis to avoid undesired S. aureus growth.

AB - Optimal transportation of bacteria is important for accurate clinical interpretation, quantitative assays, and microbiome studies. A transport medium should ideally keep the bacteria alive without supporting growth or altering the relative proportions of the constituent species. We investigated the effect of nasal mucus and mucin on the growth and survival of two Staphylococcus aureus strains in liquid Amies transport medium at room temperature and 4 °C for 14 days. The study showed that the presence of nasal mucus in microbiological samples stimulated undesired S. aureus growth at room temperature in a dose-dependent manner. These findings underscore that microbiological samples from humans and animals should be stored at 4 °C until analysis to avoid undesired S. aureus growth.

KW - Bacterial growth

KW - Clinical specimens

KW - S. aureus

KW - Transport medium

U2 - 10.1016/j.diagmicrobio.2018.07.009

DO - 10.1016/j.diagmicrobio.2018.07.009

M3 - Journal article

C2 - 30098850

AN - SCOPUS:85051070169

SN - 0732-8893

VL - 93

SP - 5

EP - 8

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 1

ER -

Carry-over of host nutrients during sampling enhances undesired growth of Staphylococcus aureus in liquid Amies transport medium

Abstract

Keywords

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