TY - JOUR
T1 - Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis
AU - Wierzbicki, Mateusz
AU - Sawosz, Ewa
AU - Grodzik, Marta
AU - Hotowy, Anna
AU - Prasek, Marta
AU - Jaworski, Slawomir
AU - Sawosz, Filip
AU - Chwalibog, André
PY - 2013/9/5
Y1 - 2013/9/5
N2 - Carbon nanoparticles, with their high biocompatibility and low toxicity, have recently been considered for biomedical applications, including antiangiogenic therapy. Critical to normal development and tumor formation, angiogenesis is the process of forming capillary blood vessels from preexisting vessels. In the present study, we evaluated the effects of diamond and graphite nanoparticles on the development of chicken embryos, as well as vascularization of the chorioallantoic membrane and heart at the morphological and molecular level. Nanoparticles did not affect either body/heart weight or serum indices of the embryos' health. However, vascularization of the heart and the density of branched vessels were significantly reduced after treatment with diamond nanoparticles and, to a lesser extent, graphite nanoparticles. Application of nanoparticles significantly downregulated gene and protein expression of the proangiogenic basic fibroblast growth factor, indicating that both diamond and graphite nanoparticles inhibit angiogenesis.
AB - Carbon nanoparticles, with their high biocompatibility and low toxicity, have recently been considered for biomedical applications, including antiangiogenic therapy. Critical to normal development and tumor formation, angiogenesis is the process of forming capillary blood vessels from preexisting vessels. In the present study, we evaluated the effects of diamond and graphite nanoparticles on the development of chicken embryos, as well as vascularization of the chorioallantoic membrane and heart at the morphological and molecular level. Nanoparticles did not affect either body/heart weight or serum indices of the embryos' health. However, vascularization of the heart and the density of branched vessels were significantly reduced after treatment with diamond nanoparticles and, to a lesser extent, graphite nanoparticles. Application of nanoparticles significantly downregulated gene and protein expression of the proangiogenic basic fibroblast growth factor, indicating that both diamond and graphite nanoparticles inhibit angiogenesis.
UR - http://www.scopus.com/inward/record.url?scp=84884548825&partnerID=8YFLogxK
U2 - 10.2147/IJN.S49745
DO - 10.2147/IJN.S49745
M3 - Journal article
C2 - 24039425
AN - SCOPUS:84884548825
SN - 1176-9114
VL - 8
SP - 3427
EP - 3435
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
IS - 1
ER -