Capture ELISA for IgM antibodies against Plasmodium falciparum glutamate rich protein

Morten Hanefeld Dziegiel, M B Borre, E Petersen, B Högh, S Jepsen, J Vuust, N Axelsen

    3 Citations (Scopus)

    Abstract

    This report describes a novel mu chain capture ELISA for the detection of IgM antibodies against a Plasmodium falciparum antigen. A fragment of the 220 kDa P. falciparum glutamate rich protein containing amino acid residues 489-1271 was expressed in E. coli as a recombinant chimeric beta-galactosidase fusion protein and used as antigen after purification and biotinylation. Specific IgM antibodies were found in 51% (39/77) of sera from adult Liberians immune to malaria. The binding of IgM antibodies was specific for the malaria portion of the fusion protein and no cross-reactivity was found in sera from patients with IgM antibodies due to other diseases. Inhibition studies with a fusion protein containing amino acid residues 816-1134 (GLURP816-1134) representing the carboxy-terminal repeat region suggested a different use of epitopes for IgM antibodies in different individuals.
    Original languageEnglish
    JournalJournal of Immunological Methods
    Volume155
    Issue number2
    Pages (from-to)207-13
    Number of pages7
    ISSN0022-1759
    Publication statusPublished - 5 Nov 1992

    Keywords

    • Animals
    • Antibodies, Protozoan
    • Antigens, Protozoan
    • Enzyme-Linked Immunosorbent Assay
    • Humans
    • Immunoglobulin M
    • Malaria, Falciparum
    • Plasmodium falciparum
    • Protozoan Proteins

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