CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins

Wolf J Jockusch; Dina Speidel; Albrecht Sigler; Jakob B Sørensen; Frederique Varoqueaux; Jeong-Seop Rhee; Nils Brose

doi:10.1016/j.cell.2007.11.002

CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins

Wolf J Jockusch, Dina Speidel, Albrecht Sigler, Jakob B Sørensen, Frederique Varoqueaux, Jeong-Seop Rhee, Nils Brose

135 Citations (Scopus)

Abstract

Before transmitter-filled synaptic vesicles can fuse with the plasma membrane upon stimulation they have to be primed to fusion competence. The regulation of this priming process controls the strength and plasticity of synaptic transmission between neurons, which in turn determines many complex brain functions. We show that CAPS-1 and CAPS-2 are essential components of the synaptic vesicle priming machinery. CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, which causes a selective impairment of fast phasic transmitter release. Increases in the intracellular Ca(2+) levels can transiently revert this defect. Our findings demonstrate that CAPS proteins generate and maintain a highly fusion competent synaptic vesicle pool that supports phasic Ca(2+) triggered release of transmitters.

Original language	English
Journal	Cell
Volume	131
Issue number	4
Pages (from-to)	796-808
Number of pages	12
ISSN	0092-8674
DOIs	https://doi.org/10.1016/j.cell.2007.11.002
Publication status	Published - 2007
Externally published	Yes

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10.1016/j.cell.2007.11.002

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@article{ee28e520fb7011de825d000ea68e967b,

title = "CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins",

abstract = "Before transmitter-filled synaptic vesicles can fuse with the plasma membrane upon stimulation they have to be primed to fusion competence. The regulation of this priming process controls the strength and plasticity of synaptic transmission between neurons, which in turn determines many complex brain functions. We show that CAPS-1 and CAPS-2 are essential components of the synaptic vesicle priming machinery. CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, which causes a selective impairment of fast phasic transmitter release. Increases in the intracellular Ca(2+) levels can transiently revert this defect. Our findings demonstrate that CAPS proteins generate and maintain a highly fusion competent synaptic vesicle pool that supports phasic Ca(2+) triggered release of transmitters.",

author = "Jockusch, {Wolf J} and Dina Speidel and Albrecht Sigler and S{\o}rensen, {Jakob B} and Frederique Varoqueaux and Jeong-Seop Rhee and Nils Brose",

note = "Keywords: Animals; Calcium; Calcium-Binding Proteins; Hippocampus; Membrane Fusion; Mice; Mice, Knockout; Nerve Tissue Proteins; Neuronal Plasticity; Neurons; Neurotransmitter Agents; Patch-Clamp Techniques; Presynaptic Terminals; Synaptic Transmission; Synaptic Vesicles",

year = "2007",

doi = "10.1016/j.cell.2007.11.002",

language = "English",

volume = "131",

pages = "796--808",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

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TY - JOUR

T1 - CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins

AU - Jockusch, Wolf J

AU - Speidel, Dina

AU - Sigler, Albrecht

AU - Sørensen, Jakob B

AU - Varoqueaux, Frederique

AU - Rhee, Jeong-Seop

AU - Brose, Nils

N1 - Keywords: Animals; Calcium; Calcium-Binding Proteins; Hippocampus; Membrane Fusion; Mice; Mice, Knockout; Nerve Tissue Proteins; Neuronal Plasticity; Neurons; Neurotransmitter Agents; Patch-Clamp Techniques; Presynaptic Terminals; Synaptic Transmission; Synaptic Vesicles

PY - 2007

Y1 - 2007

N2 - Before transmitter-filled synaptic vesicles can fuse with the plasma membrane upon stimulation they have to be primed to fusion competence. The regulation of this priming process controls the strength and plasticity of synaptic transmission between neurons, which in turn determines many complex brain functions. We show that CAPS-1 and CAPS-2 are essential components of the synaptic vesicle priming machinery. CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, which causes a selective impairment of fast phasic transmitter release. Increases in the intracellular Ca(2+) levels can transiently revert this defect. Our findings demonstrate that CAPS proteins generate and maintain a highly fusion competent synaptic vesicle pool that supports phasic Ca(2+) triggered release of transmitters.

AB - Before transmitter-filled synaptic vesicles can fuse with the plasma membrane upon stimulation they have to be primed to fusion competence. The regulation of this priming process controls the strength and plasticity of synaptic transmission between neurons, which in turn determines many complex brain functions. We show that CAPS-1 and CAPS-2 are essential components of the synaptic vesicle priming machinery. CAPS-deficient neurons contain no or very few fusion competent synaptic vesicles, which causes a selective impairment of fast phasic transmitter release. Increases in the intracellular Ca(2+) levels can transiently revert this defect. Our findings demonstrate that CAPS proteins generate and maintain a highly fusion competent synaptic vesicle pool that supports phasic Ca(2+) triggered release of transmitters.

U2 - 10.1016/j.cell.2007.11.002

DO - 10.1016/j.cell.2007.11.002

M3 - Journal article

C2 - 18022372

SN - 0092-8674

VL - 131

SP - 796

EP - 808

JO - Cell

JF - Cell

IS - 4

ER -

CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins

Abstract

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