Capillary electrophoresis of psilocybin in hallucinogenic mushrooms

Stig Pedersen-Bjergaard; Knut Einar Rasmussen

Capillary electrophoresis of psilocybin in hallucinogenic mushrooms

Stig Pedersen-Bjergaard^*, Knut Einar Rasmussen

^*Corresponding author for this work

Abstract

Capillary electrophoresis was utilized for the rapid determination of psilocybin in the hallucinogenic mushroom Psilocybe semilanceata. Following a simple two step extraction with 3.0 + 2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components utilizing capillary zone electrophoresis (CZE) with a 10 mM borate/phosphate running buffer adjusted to pH 11.5. Quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01 - 1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.and 2.5 % RSD respectively. For identity confirmation, rapid derivatization with propyl chloroformate in the methanolic extract and re-analysis was performed in order to effectively support the identification of psilocybin. In addition to this alkaloid, the method was capable also for the determination of the structurally related compounds baecystin and psilocin.

Original language	English
Journal	Journal de Pharmacie de Belgique
Volume	53
Issue number	3
Number of pages	1
ISSN	0047-2166
Publication status	Published - 1 Dec 1998

Cite this

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title = "Capillary electrophoresis of psilocybin in hallucinogenic mushrooms",

abstract = "Capillary electrophoresis was utilized for the rapid determination of psilocybin in the hallucinogenic mushroom Psilocybe semilanceata. Following a simple two step extraction with 3.0 + 2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components utilizing capillary zone electrophoresis (CZE) with a 10 mM borate/phosphate running buffer adjusted to pH 11.5. Quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01 - 1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.and 2.5 % RSD respectively. For identity confirmation, rapid derivatization with propyl chloroformate in the methanolic extract and re-analysis was performed in order to effectively support the identification of psilocybin. In addition to this alkaloid, the method was capable also for the determination of the structurally related compounds baecystin and psilocin.",

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T1 - Capillary electrophoresis of psilocybin in hallucinogenic mushrooms

AU - Pedersen-Bjergaard, Stig

AU - Rasmussen, Knut Einar

PY - 1998/12/1

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N2 - Capillary electrophoresis was utilized for the rapid determination of psilocybin in the hallucinogenic mushroom Psilocybe semilanceata. Following a simple two step extraction with 3.0 + 2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components utilizing capillary zone electrophoresis (CZE) with a 10 mM borate/phosphate running buffer adjusted to pH 11.5. Quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01 - 1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.and 2.5 % RSD respectively. For identity confirmation, rapid derivatization with propyl chloroformate in the methanolic extract and re-analysis was performed in order to effectively support the identification of psilocybin. In addition to this alkaloid, the method was capable also for the determination of the structurally related compounds baecystin and psilocin.

AB - Capillary electrophoresis was utilized for the rapid determination of psilocybin in the hallucinogenic mushroom Psilocybe semilanceata. Following a simple two step extraction with 3.0 + 2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components utilizing capillary zone electrophoresis (CZE) with a 10 mM borate/phosphate running buffer adjusted to pH 11.5. Quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01 - 1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.and 2.5 % RSD respectively. For identity confirmation, rapid derivatization with propyl chloroformate in the methanolic extract and re-analysis was performed in order to effectively support the identification of psilocybin. In addition to this alkaloid, the method was capable also for the determination of the structurally related compounds baecystin and psilocin.

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Capillary electrophoresis of psilocybin in hallucinogenic mushrooms

Abstract

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