Canine specific ELISA for coagulation factor VII

Tom Knudsen; Mads Kjelgaard-Hansen; Mikael Tranholm; Bo Wiinberg; Jes T. Clausen; Jens Jacob Hansen; Timothy C. Nichols; Marianne Kjalke; Asger Lundorff Jensen; Annemarie Thuri Kristensen

doi:10.1016/j.tvjl.2010.11.010

Canine specific ELISA for coagulation factor VII

Tom Knudsen, Mads Kjelgaard-Hansen, Mikael Tranholm, Bo Wiinberg, Jes T. Clausen, Jens Jacob Hansen, Timothy C. Nichols, Marianne Kjalke, Asger Lundorff Jensen, Annemarie Thuri Kristensen

5 Citations (Scopus)

Abstract

Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.

Original language	English
Journal	The Veterinary Journal
Volume	190
Issue number	3
Pages (from-to)	352-358
Number of pages	7
ISSN	1090-0233
DOIs	https://doi.org/10.1016/j.tvjl.2010.11.010
Publication status	Published - Dec 2011

Keywords

Former LIFE faculty

Access to Document

10.1016/j.tvjl.2010.11.010

Cite this

@article{d26a3373683d4dd99514fc96be39b6b8,

title = "Canine specific ELISA for coagulation factor VII",

abstract = "Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.",

keywords = "Former LIFE faculty, FVII deficiency, Haemostasis, Coagulation factor, ELISA, Plasma, Immunoassays",

author = "Tom Knudsen and Mads Kjelgaard-Hansen and Mikael Tranholm and Bo Wiinberg and Clausen, {Jes T.} and Hansen, {Jens Jacob} and Nichols, {Timothy C.} and Marianne Kjalke and Jensen, {Asger Lundorff} and Kristensen, {Annemarie Thuri}",

year = "2011",

month = dec,

doi = "10.1016/j.tvjl.2010.11.010",

language = "English",

volume = "190",

pages = "352--358",

journal = "The Veterinary Journal",

issn = "1090-0233",

publisher = "Elsevier",

number = "3",

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TY - JOUR

T1 - Canine specific ELISA for coagulation factor VII

AU - Knudsen, Tom

AU - Kjelgaard-Hansen, Mads

AU - Tranholm, Mikael

AU - Wiinberg, Bo

AU - Clausen, Jes T.

AU - Hansen, Jens Jacob

AU - Nichols, Timothy C.

AU - Kjalke, Marianne

AU - Jensen, Asger Lundorff

AU - Kristensen, Annemarie Thuri

PY - 2011/12

Y1 - 2011/12

N2 - Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.

AB - Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.

KW - Former LIFE faculty

KW - FVII deficiency

KW - Haemostasis

KW - Coagulation factor

KW - ELISA

KW - Plasma

KW - Immunoassays

U2 - 10.1016/j.tvjl.2010.11.010

DO - 10.1016/j.tvjl.2010.11.010

M3 - Journal article

SN - 1090-0233

VL - 190

SP - 352

EP - 358

JO - The Veterinary Journal

JF - The Veterinary Journal

IS - 3

ER -