Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting

Michelle Brønniche Christensen; Rebecca Langhorn; Amelia Goddard; Eva Bartholin Andreasen; Elena Moldal; Asta Tvarijonaviciute; Jolle Kirpenteijn; Sabrina Jakobsen; Frida Persson; Mads Kjelgaard-Hansen

doi:10.1292/jvms.12-0404

Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting

Michelle Brønniche Christensen, Rebecca Langhorn, Amelia Goddard, Eva Bartholin Andreasen, Elena Moldal, Asta Tvarijonaviciute, Jolle Kirpenteijn, Sabrina Jakobsen, Frida Persson, Mads Kjelgaard-Hansen

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Abstract

Canine serum amyloid A (SAA) is a useful diagnostic marker of systemic inflammation. A latex agglutination turbidimetric immunoassay (LAT) was validated for automated measurements. The aim of the study was to evaluate the clinical applicability of SAA measured by the LAT. SAA was measured in 7 groups of dogs with and without systemic inflammation (n=247). Overlap performance was investigated. Diagnostic performance was compared to body temperature and leukocyte markers. Clinical decision limits for SAA were estimated. In dogs with neurological, neoplastic or gastrointestinal disorders (n=143), it was investigated whether a higher proportion of SAA positive dogs could be detected in cases of complications with risk of systemic inflammation. Significantly higher concentrations of SAA were measured in dogs with (range [48.75; 5,032 mg/l]), compared to dogs without systemic inflammation [0; 56.4 mg/l]. SAA was a more sensitive and specific marker of systemic inflammation (area under the receiver-operating characteristic curve (AUC) 1.00), compared to body temperature (0.6) and segmented neutrophils (best performing leukocyte marker, 0.84). A clinical decision limit of 56.4 mg/l was established giving close to perfect discrimination between dogs with and without systemic inflammation. Higher proportions of SAApositive dogs were observed in dogs with neurological, neoplastic and gastrointestinal disorders with complications known to increase risk of systemic inflammation, compared to uncomplicated cases. The automated LAT makes SAA applicable as a relevant diagnostic marker of systemic inflammation in dogs for routine random-access real-time use in a general clinical setting.

Original language	English
Journal	Journal of Veterinary Medical Science
Volume	75
Issue number	4
Pages (from-to)	459-466
Number of pages	8
ISSN	0916-7250
DOIs	https://doi.org/10.1292/jvms.12-0404
Publication status	Published - 2013

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Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical settingFinal published version, 582 KB

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Christensen, M. B., Langhorn, R., Goddard, A., Andreasen, E. B., Moldal, E., Tvarijonaviciute, A., Kirpenteijn, J., Jakobsen, S., Persson, F., & Kjelgaard-Hansen, M. (2013). Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting. Journal of Veterinary Medical Science, 75(4), 459-466. https://doi.org/10.1292/jvms.12-0404

Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting. / Christensen, Michelle Brønniche ; Langhorn, Rebecca; Goddard, Amelia et al.
In: Journal of Veterinary Medical Science, Vol. 75, No. 4, 2013, p. 459-466.

Research output: Contribution to journal › Journal article › Research › peer-review

Christensen, MB , Langhorn, R, Goddard, A, Andreasen, EB, Moldal, E, Tvarijonaviciute, A, Kirpenteijn, J, Jakobsen, S, Persson, F & Kjelgaard-Hansen, M 2013, 'Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting', Journal of Veterinary Medical Science, vol. 75, no. 4, pp. 459-466. https://doi.org/10.1292/jvms.12-0404

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title = "Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting",

abstract = "Canine serum amyloid A (SAA) is a useful diagnostic marker of systemic inflammation. A latex agglutination turbidimetric immunoassay (LAT) was validated for automated measurements. The aim of the study was to evaluate the clinical applicability of SAA measured by the LAT. SAA was measured in 7 groups of dogs with and without systemic inflammation (n=247). Overlap performance was investigated. Diagnostic performance was compared to body temperature and leukocyte markers. Clinical decision limits for SAA were estimated. In dogs with neurological, neoplastic or gastrointestinal disorders (n=143), it was investigated whether a higher proportion of SAA positive dogs could be detected in cases of complications with risk of systemic inflammation. Significantly higher concentrations of SAA were measured in dogs with (range [48.75; 5,032 mg/l]), compared to dogs without systemic inflammation [0; 56.4 mg/l]. SAA was a more sensitive and specific marker of systemic inflammation (area under the receiver-operating characteristic curve (AUC) 1.00), compared to body temperature (0.6) and segmented neutrophils (best performing leukocyte marker, 0.84). A clinical decision limit of 56.4 mg/l was established giving close to perfect discrimination between dogs with and without systemic inflammation. Higher proportions of SAApositive dogs were observed in dogs with neurological, neoplastic and gastrointestinal disorders with complications known to increase risk of systemic inflammation, compared to uncomplicated cases. The automated LAT makes SAA applicable as a relevant diagnostic marker of systemic inflammation in dogs for routine random-access real-time use in a general clinical setting.",

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AU - Goddard, Amelia

AU - Andreasen, Eva Bartholin

AU - Moldal, Elena

AU - Tvarijonaviciute, Asta

AU - Kirpenteijn, Jolle

AU - Jakobsen, Sabrina

AU - Persson, Frida

AU - Kjelgaard-Hansen, Mads

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AB - Canine serum amyloid A (SAA) is a useful diagnostic marker of systemic inflammation. A latex agglutination turbidimetric immunoassay (LAT) was validated for automated measurements. The aim of the study was to evaluate the clinical applicability of SAA measured by the LAT. SAA was measured in 7 groups of dogs with and without systemic inflammation (n=247). Overlap performance was investigated. Diagnostic performance was compared to body temperature and leukocyte markers. Clinical decision limits for SAA were estimated. In dogs with neurological, neoplastic or gastrointestinal disorders (n=143), it was investigated whether a higher proportion of SAA positive dogs could be detected in cases of complications with risk of systemic inflammation. Significantly higher concentrations of SAA were measured in dogs with (range [48.75; 5,032 mg/l]), compared to dogs without systemic inflammation [0; 56.4 mg/l]. SAA was a more sensitive and specific marker of systemic inflammation (area under the receiver-operating characteristic curve (AUC) 1.00), compared to body temperature (0.6) and segmented neutrophils (best performing leukocyte marker, 0.84). A clinical decision limit of 56.4 mg/l was established giving close to perfect discrimination between dogs with and without systemic inflammation. Higher proportions of SAApositive dogs were observed in dogs with neurological, neoplastic and gastrointestinal disorders with complications known to increase risk of systemic inflammation, compared to uncomplicated cases. The automated LAT makes SAA applicable as a relevant diagnostic marker of systemic inflammation in dogs for routine random-access real-time use in a general clinical setting.

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DO - 10.1292/jvms.12-0404

M3 - Journal article

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JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

IS - 4

ER -

Canine serum amyloid A (SAA) measured by automated latex agglutination turbidimetry is useful for routine sensitive and specific detection of systemic inflammation in a general clinical setting

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