Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase

Amer Elias; Itay Spector; Ilana Sogolovsky-Bard; Natalia Gritsenko; Lene Rask; Yuli Mainbakh; Yael Zilberstein; Ezra Yagil; Mikhail Kolot

doi:10.1038/srep24971

Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase

Amer Elias, Itay Spector, Ilana Sogolovsky-Bard, Natalia Gritsenko, Lene Rask, Yuli Mainbakh, Yael Zilberstein, Ezra Yagil, Mikhail Kolot^*

^*Corresponding author for this work

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Abstract

Binary systems based on site-specific recombination have been used for tumor specific transcription targeting of suicide genes in animal models. In these binary systems a site specific recombinase or integrase that is expressed from a tumor specific promoter drives tumor specific expression of a cytotoxic gene. In the present study we developed a new cancer specific binary expression system activated by the Integrase (Int) of the lambdoid phage HK022. We demonstrate the validity of this system by the specific expression of a luciferase (luc) reporter in human embryonic kidney 293T (HEK293T) cells and in a lung cancer mouse model. Due to the absence viral vectors and of cytotoxicity the Int based binary system offers advantages over previously described counterparts and may therefore be developed into a safer cancer cell killing system.

Original language	English
Article number	24971
Journal	Scientific Reports
Volume	6
Number of pages	10
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep24971
Publication status	Published - 27 Apr 2016

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Cancer-specific binary expression system activated in mice by bacteriophage HK022 IntegraseFinal published version, 1.76 MBLicence: CC BY

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title = "Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase",

abstract = "Binary systems based on site-specific recombination have been used for tumor specific transcription targeting of suicide genes in animal models. In these binary systems a site specific recombinase or integrase that is expressed from a tumor specific promoter drives tumor specific expression of a cytotoxic gene. In the present study we developed a new cancer specific binary expression system activated by the Integrase (Int) of the lambdoid phage HK022. We demonstrate the validity of this system by the specific expression of a luciferase (luc) reporter in human embryonic kidney 293T (HEK293T) cells and in a lung cancer mouse model. Due to the absence viral vectors and of cytotoxicity the Int based binary system offers advantages over previously described counterparts and may therefore be developed into a safer cancer cell killing system.",

author = "Amer Elias and Itay Spector and Ilana Sogolovsky-Bard and Natalia Gritsenko and Lene Rask and Yuli Mainbakh and Yael Zilberstein and Ezra Yagil and Mikhail Kolot",

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T1 - Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase

AU - Elias, Amer

AU - Spector, Itay

AU - Sogolovsky-Bard, Ilana

AU - Gritsenko, Natalia

AU - Rask, Lene

AU - Mainbakh, Yuli

AU - Zilberstein, Yael

AU - Yagil, Ezra

AU - Kolot, Mikhail

PY - 2016/4/27

Y1 - 2016/4/27

N2 - Binary systems based on site-specific recombination have been used for tumor specific transcription targeting of suicide genes in animal models. In these binary systems a site specific recombinase or integrase that is expressed from a tumor specific promoter drives tumor specific expression of a cytotoxic gene. In the present study we developed a new cancer specific binary expression system activated by the Integrase (Int) of the lambdoid phage HK022. We demonstrate the validity of this system by the specific expression of a luciferase (luc) reporter in human embryonic kidney 293T (HEK293T) cells and in a lung cancer mouse model. Due to the absence viral vectors and of cytotoxicity the Int based binary system offers advantages over previously described counterparts and may therefore be developed into a safer cancer cell killing system.

AB - Binary systems based on site-specific recombination have been used for tumor specific transcription targeting of suicide genes in animal models. In these binary systems a site specific recombinase or integrase that is expressed from a tumor specific promoter drives tumor specific expression of a cytotoxic gene. In the present study we developed a new cancer specific binary expression system activated by the Integrase (Int) of the lambdoid phage HK022. We demonstrate the validity of this system by the specific expression of a luciferase (luc) reporter in human embryonic kidney 293T (HEK293T) cells and in a lung cancer mouse model. Due to the absence viral vectors and of cytotoxicity the Int based binary system offers advantages over previously described counterparts and may therefore be developed into a safer cancer cell killing system.

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DO - 10.1038/srep24971

M3 - Journal article

C2 - 27117628

AN - SCOPUS:84964869898

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 24971

ER -

Cancer-specific binary expression system activated in mice by bacteriophage HK022 Integrase

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