Can a Charged Surfactant Unfold an Uncharged Protein?

Casper Højgaard; Henrik Vinther Sørensen; Jan Skov Pedersen; Jakob Rahr Winther; Daniel Erik Otzen

doi:10.1016/j.bpj.2018.10.022

Can a Charged Surfactant Unfold an Uncharged Protein?

Casper Højgaard, Henrik Vinther Sørensen, Jan Skov Pedersen, Jakob Rahr Winther, Daniel Erik Otzen^*

^*Corresponding author for this work

Biomolecular Sciences

9 Citations (Scopus)

Abstract

Does sodium dodecyl sulfate (SDS) denature proteins through electrostatic SDS-protein interactions? We show that a protein completely lacking charged side chains is unfolded by SDS in a manner similar to charged proteins, revealing that formal protein charges are not required for SDS-induced protein unfolding or binding.

Original language	English
Journal	Biophysical Journal
Volume	115
Issue number	11
Pages (from-to)	2081-2086
Number of pages	6
ISSN	0006-3495
DOIs	https://doi.org/10.1016/j.bpj.2018.10.022
Publication status	Published - 2018

Access to Document

10.1016/j.bpj.2018.10.022

http://www.cell.com/article/S0006349518312116/pdf

Cite this

Højgaard, C., Sørensen, H. V., Pedersen, J. S., Winther, J. R., & Otzen, D. E. (2018). Can a Charged Surfactant Unfold an Uncharged Protein? Biophysical Journal, 115(11), 2081-2086. https://doi.org/10.1016/j.bpj.2018.10.022

@article{f063d7efbc0e461b91b13c1957c72d88,

title = "Can a Charged Surfactant Unfold an Uncharged Protein?",

abstract = "Does sodium dodecyl sulfate (SDS) denature proteins through electrostatic SDS-protein interactions? We show that a protein completely lacking charged side chains is unfolded by SDS in a manner similar to charged proteins, revealing that formal protein charges are not required for SDS-induced protein unfolding or binding.",

author = "Casper H{\o}jgaard and S{\o}rensen, {Henrik Vinther} and Pedersen, {Jan Skov} and Winther, {Jakob Rahr} and Otzen, {Daniel Erik}",

year = "2018",

doi = "10.1016/j.bpj.2018.10.022",

language = "English",

volume = "115",

pages = "2081--2086",

journal = "Biophysical Journal",

issn = "0006-3495",