Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris)

Marie Pedersen; Bernadette Schoket; Roger W Godschalk; John Wright; Hans von Stedingk; Margareta Törnqvist; Jordi Sunyer; Jeanette K Nielsen; Domenico F Merlo; Michelle A Mendez; Helle M Meltzer; Viktória Lukács; Anette Landström; Soterios A Kyrtopoulos; Katalin Kovács; Lisbeth E. Knudsen; Margaretha Haugen; Laura J Hardie; Kristine B Gützkow; Sarah Fleming; Eleni Fthenou; Peter B Farmer; Aina Espinosa; Leda Chatzi; Gunnar Brunborg; Nigel J Brady; Maria Botsivali; Khelifa Arab; Lívia Anna; Jan Alexander; Silvia Agramunt; Jos C Kleinjans; Dan Segerbäck; Manolis Kogevinas

doi:10.1289/ehp.1206333

Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris)

Marie Pedersen, Bernadette Schoket, Roger W Godschalk, John Wright, Hans von Stedingk, Margareta Törnqvist, Jordi Sunyer, Jeanette K Nielsen, Domenico F Merlo, Michelle A Mendez, Helle M Meltzer, Viktória Lukács, Anette Landström, Soterios A Kyrtopoulos, Katalin Kovács, Lisbeth E. Knudsen, Margaretha Haugen, Laura J Hardie, Kristine B Gützkow, Sarah FlemingEleni Fthenou, Peter B Farmer, Aina Espinosa, Leda Chatzi, Gunnar Brunborg, Nigel J Brady, Maria Botsivali, Khelifa Arab, Lívia Anna, Jan Alexander, Silvia Agramunt, Jos C Kleinjans, Dan Segerbäck, Manolis Kogevinas

27 Citations (Scopus)

Abstract

Background: Tobacco-smoke, airborne, and dietary exposures to polycyclic aromatic hydrocarbons (PAHs) have been associated with reduced prenatal growth. Evidence from biomarker-based studies of low-exposed populations is limited. Bulky DNA adducts in cord blood reflect the prenatal effective dose to several genotoxic agents including PAHs. Objectives: We estimated the association between bulky DNA adduct levels and birth weight in a multicenter study and examined modification of this association by maternal intake of fruits and vegetables during pregnancy. Methods: Pregnant women from Denmark, England, Greece, Norway, and Spain were recruited in 2006-2010. Adduct levels were measured by the ³²P-postlabeling technique in white blood cells from 229 mothers and 612 newborns. Maternal diet was examined through questionnaires. Results: Adduct levels in maternal and cord blood samples were similar and positively correlated (median, 12.1 vs. 11.4 adducts in 10⁸ nucleotides; Spearman rank correlation coefficient = 0.66, p < 0.001). Cord blood adduct levels were negatively associated with birth weight, with an estimated difference in mean birth weight of -129 g (95% CI: -233, -25 g) for infants in the highest versus lowest tertile of adducts. The negative association with birth weight was limited to births in Norway, Denmark, and England, the countries with the lowest adduct levels, and was more pronounced in births to mothers with low intake of fruits and vegetables (-248 g; 95% CI: -405, -92 g) compared with those with high intake (-58 g; 95% CI: -206, 90 g). Conclusions: Maternal exposure to genotoxic agents that induce the formation of bulky DNA adducts may affect intrauterine growth. Maternal fruit and vegetable consumption may be protective.

Original language	English
Journal	Environmental Health Perspectives
Volume	121
Issue number	10
Pages (from-to)	1200-1206
Number of pages	7
ISSN	0091-6765
DOIs	https://doi.org/10.1289/ehp.1206333
Publication status	Published - Oct 2013

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Pedersen, M., Schoket, B., Godschalk, R. W., Wright, J., von Stedingk, H., Törnqvist, M., Sunyer, J., Nielsen, J. K., Merlo, D. F., Mendez, M. A., Meltzer, H. M., Lukács, V., Landström, A., Kyrtopoulos, S. A., Kovács, K., Knudsen, L. E., Haugen, M., Hardie, L. J., Gützkow, K. B., ... Kogevinas, M. (2013). Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris). Environmental Health Perspectives, 121(10), 1200-1206. https://doi.org/10.1289/ehp.1206333

Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris). / Pedersen, Marie; Schoket, Bernadette; Godschalk, Roger W et al.

In: Environmental Health Perspectives, Vol. 121, No. 10, 10.2013, p. 1200-1206.

Research output: Contribution to journal › Journal article › Research › peer-review

Pedersen, M, Schoket, B, Godschalk, RW, Wright, J, von Stedingk, H, Törnqvist, M, Sunyer, J, Nielsen, JK, Merlo, DF, Mendez, MA, Meltzer, HM, Lukács, V, Landström, A, Kyrtopoulos, SA, Kovács, K, Knudsen, LE, Haugen, M, Hardie, LJ, Gützkow, KB, Fleming, S, Fthenou, E, Farmer, PB, Espinosa, A, Chatzi, L, Brunborg, G, Brady, NJ, Botsivali, M, Arab, K, Anna, L, Alexander, J, Agramunt, S, Kleinjans, JC, Segerbäck, D & Kogevinas, M 2013, 'Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris)', Environmental Health Perspectives, vol. 121, no. 10, pp. 1200-1206. https://doi.org/10.1289/ehp.1206333

@article{e500e3404f1f4cc4b8e68a21785dcbf8,

title = "Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris)",

abstract = "Background: Tobacco-smoke, airborne, and dietary exposures to polycyclic aromatic hydrocarbons (PAHs) have been associated with reduced prenatal growth. Evidence from biomarker-based studies of low-exposed populations is limited. Bulky DNA adducts in cord blood reflect the prenatal effective dose to several genotoxic agents including PAHs. Objectives: We estimated the association between bulky DNA adduct levels and birth weight in a multicenter study and examined modification of this association by maternal intake of fruits and vegetables during pregnancy. Methods: Pregnant women from Denmark, England, Greece, Norway, and Spain were recruited in 2006-2010. Adduct levels were measured by the 32P-postlabeling technique in white blood cells from 229 mothers and 612 newborns. Maternal diet was examined through questionnaires. Results: Adduct levels in maternal and cord blood samples were similar and positively correlated (median, 12.1 vs. 11.4 adducts in 108 nucleotides; Spearman rank correlation coefficient = 0.66, p < 0.001). Cord blood adduct levels were negatively associated with birth weight, with an estimated difference in mean birth weight of -129 g (95% CI: -233, -25 g) for infants in the highest versus lowest tertile of adducts. The negative association with birth weight was limited to births in Norway, Denmark, and England, the countries with the lowest adduct levels, and was more pronounced in births to mothers with low intake of fruits and vegetables (-248 g; 95% CI: -405, -92 g) compared with those with high intake (-58 g; 95% CI: -206, 90 g). Conclusions: Maternal exposure to genotoxic agents that induce the formation of bulky DNA adducts may affect intrauterine growth. Maternal fruit and vegetable consumption may be protective.",

author = "Marie Pedersen and Bernadette Schoket and Godschalk, {Roger W} and John Wright and {von Stedingk}, Hans and Margareta T{\"o}rnqvist and Jordi Sunyer and Nielsen, {Jeanette K} and Merlo, {Domenico F} and Mendez, {Michelle A} and Meltzer, {Helle M} and Vikt{\'o}ria Luk{\'a}cs and Anette Landstr{\"o}m and Kyrtopoulos, {Soterios A} and Katalin Kov{\'a}cs and Knudsen, {Lisbeth E.} and Margaretha Haugen and Hardie, {Laura J} and G{\"u}tzkow, {Kristine B} and Sarah Fleming and Eleni Fthenou and Farmer, {Peter B} and Aina Espinosa and Leda Chatzi and Gunnar Brunborg and Brady, {Nigel J} and Maria Botsivali and Khelifa Arab and L{\'i}via Anna and Jan Alexander and Silvia Agramunt and Kleinjans, {Jos C} and Dan Segerb{\"a}ck and Manolis Kogevinas",

year = "2013",

month = oct,

doi = "10.1289/ehp.1206333",

language = "English",

volume = "121",

pages = "1200--1206",

journal = "Environmental Health Perspectives",

issn = "0091-6765",

publisher = "National Institute of Environmental Health Sciences",

number = "10",

}

TY - JOUR

T1 - Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris)

AU - Pedersen, Marie

AU - Schoket, Bernadette

AU - Godschalk, Roger W

AU - Wright, John

AU - von Stedingk, Hans

AU - Törnqvist, Margareta

AU - Sunyer, Jordi

AU - Nielsen, Jeanette K

AU - Merlo, Domenico F

AU - Mendez, Michelle A

AU - Meltzer, Helle M

AU - Lukács, Viktória

AU - Landström, Anette

AU - Kyrtopoulos, Soterios A

AU - Kovács, Katalin

AU - Knudsen, Lisbeth E.

AU - Haugen, Margaretha

AU - Hardie, Laura J

AU - Gützkow, Kristine B

AU - Fleming, Sarah

AU - Fthenou, Eleni

AU - Farmer, Peter B

AU - Espinosa, Aina

AU - Chatzi, Leda

AU - Brunborg, Gunnar

AU - Brady, Nigel J

AU - Botsivali, Maria

AU - Arab, Khelifa

AU - Anna, Lívia

AU - Alexander, Jan

AU - Agramunt, Silvia

AU - Kleinjans, Jos C

AU - Segerbäck, Dan

AU - Kogevinas, Manolis

PY - 2013/10

Y1 - 2013/10

N2 - Background: Tobacco-smoke, airborne, and dietary exposures to polycyclic aromatic hydrocarbons (PAHs) have been associated with reduced prenatal growth. Evidence from biomarker-based studies of low-exposed populations is limited. Bulky DNA adducts in cord blood reflect the prenatal effective dose to several genotoxic agents including PAHs. Objectives: We estimated the association between bulky DNA adduct levels and birth weight in a multicenter study and examined modification of this association by maternal intake of fruits and vegetables during pregnancy. Methods: Pregnant women from Denmark, England, Greece, Norway, and Spain were recruited in 2006-2010. Adduct levels were measured by the 32P-postlabeling technique in white blood cells from 229 mothers and 612 newborns. Maternal diet was examined through questionnaires. Results: Adduct levels in maternal and cord blood samples were similar and positively correlated (median, 12.1 vs. 11.4 adducts in 108 nucleotides; Spearman rank correlation coefficient = 0.66, p < 0.001). Cord blood adduct levels were negatively associated with birth weight, with an estimated difference in mean birth weight of -129 g (95% CI: -233, -25 g) for infants in the highest versus lowest tertile of adducts. The negative association with birth weight was limited to births in Norway, Denmark, and England, the countries with the lowest adduct levels, and was more pronounced in births to mothers with low intake of fruits and vegetables (-248 g; 95% CI: -405, -92 g) compared with those with high intake (-58 g; 95% CI: -206, 90 g). Conclusions: Maternal exposure to genotoxic agents that induce the formation of bulky DNA adducts may affect intrauterine growth. Maternal fruit and vegetable consumption may be protective.

AB - Background: Tobacco-smoke, airborne, and dietary exposures to polycyclic aromatic hydrocarbons (PAHs) have been associated with reduced prenatal growth. Evidence from biomarker-based studies of low-exposed populations is limited. Bulky DNA adducts in cord blood reflect the prenatal effective dose to several genotoxic agents including PAHs. Objectives: We estimated the association between bulky DNA adduct levels and birth weight in a multicenter study and examined modification of this association by maternal intake of fruits and vegetables during pregnancy. Methods: Pregnant women from Denmark, England, Greece, Norway, and Spain were recruited in 2006-2010. Adduct levels were measured by the 32P-postlabeling technique in white blood cells from 229 mothers and 612 newborns. Maternal diet was examined through questionnaires. Results: Adduct levels in maternal and cord blood samples were similar and positively correlated (median, 12.1 vs. 11.4 adducts in 108 nucleotides; Spearman rank correlation coefficient = 0.66, p < 0.001). Cord blood adduct levels were negatively associated with birth weight, with an estimated difference in mean birth weight of -129 g (95% CI: -233, -25 g) for infants in the highest versus lowest tertile of adducts. The negative association with birth weight was limited to births in Norway, Denmark, and England, the countries with the lowest adduct levels, and was more pronounced in births to mothers with low intake of fruits and vegetables (-248 g; 95% CI: -405, -92 g) compared with those with high intake (-58 g; 95% CI: -206, 90 g). Conclusions: Maternal exposure to genotoxic agents that induce the formation of bulky DNA adducts may affect intrauterine growth. Maternal fruit and vegetable consumption may be protective.

U2 - 10.1289/ehp.1206333

DO - 10.1289/ehp.1206333

M3 - Journal article

C2 - 23906905

SN - 0091-6765

VL - 121

SP - 1200

EP - 1206

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

IS - 10

ER -

Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris)

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