BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation

TY - JOUR

T1 - BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation

AU - Feng, Yunpeng

AU - Vlassis, Arsenios

AU - Roques, Céline

AU - Lalonde, Marie Eve

AU - Gonzalez Aguilera, Cristina

AU - Lambert, Jean Philippe

AU - Lee, Sung-Bau

AU - Zhao, Xiaobei

AU - Alabert, Constance

AU - Johansen, Jens Vilstrup

AU - Paquet, Eric

AU - Yang, Xiang Jiao

AU - Gingras, Anne Claude

AU - Côté, Jacques

AU - Groth, Anja

PY - 2016/1/1

Y1 - 2016/1/1

N2 - During DNA replication, thousands of replication origins are activated across the genome. Chromatin architecture contributes to origin specification and usage, yet it remains unclear which chromatin features impact on DNA replication. Here, we perform a RNAi screen for chromatin regulators implicated in replication control by measuring RPA accumulation upon replication stress. We identify six factors required for normal rates of DNA replication and characterize a function of the bromodomain and PHD finger-containing protein 3 (BRPF3) in replication initiation. BRPF3 forms a complex with HBO1 that specifically acetylates histone H3K14, and genomewide analysis shows high enrichment of BRPF3, HBO1 and H3K14ac at ORC1-binding sites and replication origins found in the vicinity of TSSs. Consistent with this, BRPF3 is necessary for H3K14ac at selected origins and efficient origin activation. CDC45 recruitment, but not MCM2-7 loading, is impaired in BRPF3-depleted cells, identifying a BRPF3-dependent function of HBO1 in origin activation that is complementary to its role in licencing. We thus propose that BRPF3-HBO1 acetylation of histone H3K14 around TSS facilitates efficient activation of nearby replication origins. Synopsis A distinct origin activation role of HBO1 acetyltransferase provides new insight into how demarcation of chromatin surrounding transcription start sites affects the regulation of nearby replication origins. siRNA screen identifies chromatin regulators important for DNA replication, including the scaffold protein BRPF3. BRPF3 regulates origin firing by directing the acetyltransferase HBO1 to target histone H3K14 in chromatin surrounding replication origins. HBO1-BRPF3 complex function in origin activation is separate from and complementary to HBO1-JADE1 function in origin licensing. Reduced origin activation upon BRPF3 depletion protects cells against replication stress-induced DNA damage. An RNAi screen for chromatin regulators of replication control reveals an origin activation role of HBO1 acetyltransferase role that is separate from its function in origin licensing.

AB - During DNA replication, thousands of replication origins are activated across the genome. Chromatin architecture contributes to origin specification and usage, yet it remains unclear which chromatin features impact on DNA replication. Here, we perform a RNAi screen for chromatin regulators implicated in replication control by measuring RPA accumulation upon replication stress. We identify six factors required for normal rates of DNA replication and characterize a function of the bromodomain and PHD finger-containing protein 3 (BRPF3) in replication initiation. BRPF3 forms a complex with HBO1 that specifically acetylates histone H3K14, and genomewide analysis shows high enrichment of BRPF3, HBO1 and H3K14ac at ORC1-binding sites and replication origins found in the vicinity of TSSs. Consistent with this, BRPF3 is necessary for H3K14ac at selected origins and efficient origin activation. CDC45 recruitment, but not MCM2-7 loading, is impaired in BRPF3-depleted cells, identifying a BRPF3-dependent function of HBO1 in origin activation that is complementary to its role in licencing. We thus propose that BRPF3-HBO1 acetylation of histone H3K14 around TSS facilitates efficient activation of nearby replication origins. Synopsis A distinct origin activation role of HBO1 acetyltransferase provides new insight into how demarcation of chromatin surrounding transcription start sites affects the regulation of nearby replication origins. siRNA screen identifies chromatin regulators important for DNA replication, including the scaffold protein BRPF3. BRPF3 regulates origin firing by directing the acetyltransferase HBO1 to target histone H3K14 in chromatin surrounding replication origins. HBO1-BRPF3 complex function in origin activation is separate from and complementary to HBO1-JADE1 function in origin licensing. Reduced origin activation upon BRPF3 depletion protects cells against replication stress-induced DNA damage. An RNAi screen for chromatin regulators of replication control reveals an origin activation role of HBO1 acetyltransferase role that is separate from its function in origin licensing.

KW - BRPF

KW - DNA replication

KW - H3K14ac

KW - HBO1

KW - Origin activation

U2 - 10.15252/embj.201591293

DO - 10.15252/embj.201591293

M3 - Journal article

C2 - 26620551

SN - 0261-4189

VL - 35

SP - 176

EP - 192

JO - EMBO Journal

JF - EMBO Journal

IS - 2

ER -