Bone regeneration in standardized bone defects with autografts or bone substitutes in combination with platelet concentrate: a histologic and histomorphometric study in the mandibles of minipigs

TY - JOUR

T1 - Bone regeneration in standardized bone defects with autografts or bone substitutes in combination with platelet concentrate

T2 - a histologic and histomorphometric study in the mandibles of minipigs

AU - Jensen, Simon Storgård

AU - Broggini, Nina

AU - Weibrich, Gernot

AU - Hjôrting-Hansen, Erik

AU - Schenk, Robert

AU - Buser, Daniel

PY - 2005/11/9

Y1 - 2005/11/9

N2 - PURPOSE: To evaluate the effect of the addition of platelet concentrate (PC) to autografts or bone substitutes on bone regeneration in standardized bone defects.MATERIALS AND METHODS: Three standardized bone defects were prepared in both mandibular angles of 12 adult minipigs. The defects were grafted with autograft, anorganic bovine bone, or synthetic beta-tricalcium phosphate (beta-TCP). PC was added to only 1 side. The animals were divided into 4 groups, which were sacrificed at 4 different time points (1, 2, 4, and 8 weeks) for histologic and histomorphometric analysis. The concentrations of platelets and growth factors were measured to identify correlation to the histologic and histomorphometric results.RESULTS: No correlation was found between platelet count in whole blood and platelet count in PC (r(p) = 0.36). Furthermore, no correlation could be demonstrated between the platelet count of the PC and the concentrations of PDGF-AB (r(p) = -0.27) and TGF-beta (r(p) = 0.34). There were no signs of a stimulating effect of PC on bone formation in combination with autografts or bone substitutes at any time point (P = .89). Addition of PC did not alter the pattern of graft degradation.DISCUSSION: The present study underlines the need for further investigation to identify the optimal concentrations of platelets and combinations of growth factors to achieve a predictable stimulatory effect on bone regeneration. One of the first steps to achieve this goal will be the development of a reliable method for the procurement of PC.CONCLUSION: PC had no impact on bone formation and graft degradation in standardized bone defects in the mandibles of minipigs.

AB - PURPOSE: To evaluate the effect of the addition of platelet concentrate (PC) to autografts or bone substitutes on bone regeneration in standardized bone defects.MATERIALS AND METHODS: Three standardized bone defects were prepared in both mandibular angles of 12 adult minipigs. The defects were grafted with autograft, anorganic bovine bone, or synthetic beta-tricalcium phosphate (beta-TCP). PC was added to only 1 side. The animals were divided into 4 groups, which were sacrificed at 4 different time points (1, 2, 4, and 8 weeks) for histologic and histomorphometric analysis. The concentrations of platelets and growth factors were measured to identify correlation to the histologic and histomorphometric results.RESULTS: No correlation was found between platelet count in whole blood and platelet count in PC (r(p) = 0.36). Furthermore, no correlation could be demonstrated between the platelet count of the PC and the concentrations of PDGF-AB (r(p) = -0.27) and TGF-beta (r(p) = 0.34). There were no signs of a stimulating effect of PC on bone formation in combination with autografts or bone substitutes at any time point (P = .89). Addition of PC did not alter the pattern of graft degradation.DISCUSSION: The present study underlines the need for further investigation to identify the optimal concentrations of platelets and combinations of growth factors to achieve a predictable stimulatory effect on bone regeneration. One of the first steps to achieve this goal will be the development of a reliable method for the procurement of PC.CONCLUSION: PC had no impact on bone formation and graft degradation in standardized bone defects in the mandibles of minipigs.

KW - Analysis of Variance

KW - Animals

KW - Blood Platelets

KW - Bone Regeneration

KW - Bone Substitutes

KW - Bone Transplantation

KW - Calcium Phosphates

KW - Cattle

KW - Guided Tissue Regeneration, Periodontal/methods

KW - Mandible/surgery

KW - Minerals

KW - Platelet Count

KW - Platelet-Derived Growth Factor/analysis

KW - Random Allocation

KW - Regression Analysis

KW - Swine

KW - Swine, Miniature

KW - Transforming Growth Factor beta/analysis

M3 - Journal article

C2 - 16274143

SN - 0882-2786

VL - 20

SP - 703

EP - 712

JO - International Journal of Oral and Maxillofacial Implants

JF - International Journal of Oral and Maxillofacial Implants

IS - 5

ER -