Blood-retinal barrier glycerol permeability in diabetic macular edema and healthy eyes: estimations from macular volume changes after peroral glycerol

Dorte Nellemann Thornit; Carl Martin Vinten; Birgit Sander; Henrik Lund-Andersen; Morten la Cour; Dorte Nellemann Thornit; Carl Martin Vinten; Birgit Sander; Henrik Lund-Andersen; Morten la Cour

doi:10.1167/iovs.09-4172

Blood-retinal barrier glycerol permeability in diabetic macular edema and healthy eyes: estimations from macular volume changes after peroral glycerol

Dorte Nellemann Thornit, Carl Martin Vinten, Birgit Sander, Henrik Lund-Andersen, Morten la Cour, Dorte Nellemann Thornit, Carl Martin Vinten, Birgit Sander, Henrik Lund-Andersen, Morten la Cour

12 Citations (Scopus)

Abstract

PURPOSE. To compare the changes in macular volume (MV) between healthy subjects and patients with diabetic macular edema (DME) after an osmotic load and to determine the glycerol permeability (P_gly) of the blood-retinal barrier (BRB). METHODS. In this unmasked study, 13 patients with DME and 5 healthy control subjects ingested a glycerol solution (0.57 g/mL) of 3 mL/kg body weight (maximum, 250 mL). Subsequently, the MV determined by the retinal maps provided by the optical coherence tomography (OCT) fast macular thickness protocol was monitored at 12 time points for 180 minutes. A mathematical model of glycerol and osmotic water movements across the BRB was constructed to estimate P_gly. RESULTS. Median MV decreased from 7.30 mm³3 (range, 6.68- 7.35) to the maximum median ΔMV of -0.30 mm³3 (25%-75% quartile: -0.34 to -0.25) in the healthy volunteers and from 7.97 mm³3 (range, 6.85-9.89) to ΔMV of _0.14 mm³3 (25%-75% quartile: -0.19 to -0.08) in the diabetic group (intergroup difference: P< 0.05). Pgly was 6.1 × 10^-6 (SE 1.8 × 10^-6) and 74 × 10^-6 (SE 42 × 10^-6) cm/s in the healthy and diabetic participants, respectively (P × 0.0001). No rebound phenomenon was observed in either group. CONCLUSIONS. The maximum reduction in MV was doubled in the healthy group compared with the diabetic group, whereas the glycerol permeability was 12 times higher in the diabetic participants. These findings confirm the paradigm of BRB breakdown in DME, but also suggest a novel procedure for the determination of retinal permeability to various agents, which is independent of the vitreous condition (ClinicalTrials.gov number, NCT00333671).

Original language	English
Journal	Investigative Ophthalmology & Visual Science
Volume	51
Issue number	6
Pages (from-to)	2827-34
Number of pages	8
ISSN	0146-0404
DOIs	https://doi.org/10.1167/iovs.09-4172
Publication status	Published - 1 Jun 2010

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10.1167/iovs.09-4172

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Thornit, D. N., Vinten, C. M., Sander, B., Lund-Andersen, H., la Cour, M., Thornit, D. N., Vinten, C. M., Sander, B., Lund-Andersen, H., & la Cour, M. (2010). Blood-retinal barrier glycerol permeability in diabetic macular edema and healthy eyes: estimations from macular volume changes after peroral glycerol. Investigative Ophthalmology & Visual Science, 51(6), 2827-34. https://doi.org/10.1167/iovs.09-4172

Blood-retinal barrier glycerol permeability in diabetic macular edema and healthy eyes: estimations from macular volume changes after peroral glycerol. / Thornit, Dorte Nellemann; Vinten, Carl Martin; Sander, Birgit et al.
In: Investigative Ophthalmology & Visual Science, Vol. 51, No. 6, 01.06.2010, p. 2827-34.

Research output: Contribution to journal › Journal article › Research › peer-review

Thornit, DN, Vinten, CM, Sander, B, Lund-Andersen, H, la Cour, M, Thornit, DN, Vinten, CM, Sander, B, Lund-Andersen, H & la Cour, M 2010, 'Blood-retinal barrier glycerol permeability in diabetic macular edema and healthy eyes: estimations from macular volume changes after peroral glycerol', Investigative Ophthalmology & Visual Science, vol. 51, no. 6, pp. 2827-34. https://doi.org/10.1167/iovs.09-4172

@article{aa07865088cc11df928f000ea68e967b,

title = "Blood-retinal barrier glycerol permeability in diabetic macular edema and healthy eyes: estimations from macular volume changes after peroral glycerol",

abstract = "PURPOSE. To compare the changes in macular volume (MV) between healthy subjects and patients with diabetic macular edema (DME) after an osmotic load and to determine the glycerol permeability (Pgly) of the blood-retinal barrier (BRB). METHODS. In this unmasked study, 13 patients with DME and 5 healthy control subjects ingested a glycerol solution (0.57 g/mL) of 3 mL/kg body weight (maximum, 250 mL). Subsequently, the MV determined by the retinal maps provided by the optical coherence tomography (OCT) fast macular thickness protocol was monitored at 12 time points for 180 minutes. A mathematical model of glycerol and osmotic water movements across the BRB was constructed to estimate Pgly. RESULTS. Median MV decreased from 7.30 mm33 (range, 6.68- 7.35) to the maximum median ΔMV of -0.30 mm33 (25%-75% quartile: -0.34 to -0.25) in the healthy volunteers and from 7.97 mm33 (range, 6.85-9.89) to ΔMV of _0.14 mm33 (25%-75% quartile: -0.19 to -0.08) in the diabetic group (intergroup difference: P< 0.05). Pgly was 6.1 × 10-6 (SE 1.8 × 10-6) and 74 × 10-6 (SE 42 × 10-6) cm/s in the healthy and diabetic participants, respectively (P × 0.0001). No rebound phenomenon was observed in either group. CONCLUSIONS. The maximum reduction in MV was doubled in the healthy group compared with the diabetic group, whereas the glycerol permeability was 12 times higher in the diabetic participants. These findings confirm the paradigm of BRB breakdown in DME, but also suggest a novel procedure for the determination of retinal permeability to various agents, which is independent of the vitreous condition (ClinicalTrials.gov number, NCT00333671).",

author = "Thornit, {Dorte Nellemann} and Vinten, {Carl Martin} and Birgit Sander and Henrik Lund-Andersen and {la Cour}, Morten and Thornit, {Dorte Nellemann} and Vinten, {Carl Martin} and Birgit Sander and Henrik Lund-Andersen and {la Cour}, Morten",

note = "Keywords: Aged; Biological Transport; Blood Pressure; Blood-Retinal Barrier; Body Water; Capillary Permeability; Creatinine; Diabetic Retinopathy; Female; Glycerol; Hemoglobin A, Glycosylated; Humans; Macular Edema; Male; Middle Aged; Models, Theoretical; Osmolar Concentration; Prospective Studies; Retina; Tomography, Optical Coherence; Visual Acuity",

year = "2010",

month = jun,

day = "1",

doi = "10.1167/iovs.09-4172",

language = "English",

volume = "51",

pages = "2827--34",

journal = "Investigative Ophthalmology & Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology",

number = "6",

}

TY - JOUR

T1 - Blood-retinal barrier glycerol permeability in diabetic macular edema and healthy eyes: estimations from macular volume changes after peroral glycerol

AU - Thornit, Dorte Nellemann

AU - Vinten, Carl Martin

AU - Sander, Birgit

AU - Lund-Andersen, Henrik

AU - la Cour, Morten

AU - Thornit, Dorte Nellemann

AU - Vinten, Carl Martin

AU - Sander, Birgit

AU - Lund-Andersen, Henrik

AU - la Cour, Morten

N1 - Keywords: Aged; Biological Transport; Blood Pressure; Blood-Retinal Barrier; Body Water; Capillary Permeability; Creatinine; Diabetic Retinopathy; Female; Glycerol; Hemoglobin A, Glycosylated; Humans; Macular Edema; Male; Middle Aged; Models, Theoretical; Osmolar Concentration; Prospective Studies; Retina; Tomography, Optical Coherence; Visual Acuity

PY - 2010/6/1

Y1 - 2010/6/1

N2 - PURPOSE. To compare the changes in macular volume (MV) between healthy subjects and patients with diabetic macular edema (DME) after an osmotic load and to determine the glycerol permeability (Pgly) of the blood-retinal barrier (BRB). METHODS. In this unmasked study, 13 patients with DME and 5 healthy control subjects ingested a glycerol solution (0.57 g/mL) of 3 mL/kg body weight (maximum, 250 mL). Subsequently, the MV determined by the retinal maps provided by the optical coherence tomography (OCT) fast macular thickness protocol was monitored at 12 time points for 180 minutes. A mathematical model of glycerol and osmotic water movements across the BRB was constructed to estimate Pgly. RESULTS. Median MV decreased from 7.30 mm33 (range, 6.68- 7.35) to the maximum median ΔMV of -0.30 mm33 (25%-75% quartile: -0.34 to -0.25) in the healthy volunteers and from 7.97 mm33 (range, 6.85-9.89) to ΔMV of _0.14 mm33 (25%-75% quartile: -0.19 to -0.08) in the diabetic group (intergroup difference: P< 0.05). Pgly was 6.1 × 10-6 (SE 1.8 × 10-6) and 74 × 10-6 (SE 42 × 10-6) cm/s in the healthy and diabetic participants, respectively (P × 0.0001). No rebound phenomenon was observed in either group. CONCLUSIONS. The maximum reduction in MV was doubled in the healthy group compared with the diabetic group, whereas the glycerol permeability was 12 times higher in the diabetic participants. These findings confirm the paradigm of BRB breakdown in DME, but also suggest a novel procedure for the determination of retinal permeability to various agents, which is independent of the vitreous condition (ClinicalTrials.gov number, NCT00333671).

AB - PURPOSE. To compare the changes in macular volume (MV) between healthy subjects and patients with diabetic macular edema (DME) after an osmotic load and to determine the glycerol permeability (Pgly) of the blood-retinal barrier (BRB). METHODS. In this unmasked study, 13 patients with DME and 5 healthy control subjects ingested a glycerol solution (0.57 g/mL) of 3 mL/kg body weight (maximum, 250 mL). Subsequently, the MV determined by the retinal maps provided by the optical coherence tomography (OCT) fast macular thickness protocol was monitored at 12 time points for 180 minutes. A mathematical model of glycerol and osmotic water movements across the BRB was constructed to estimate Pgly. RESULTS. Median MV decreased from 7.30 mm33 (range, 6.68- 7.35) to the maximum median ΔMV of -0.30 mm33 (25%-75% quartile: -0.34 to -0.25) in the healthy volunteers and from 7.97 mm33 (range, 6.85-9.89) to ΔMV of _0.14 mm33 (25%-75% quartile: -0.19 to -0.08) in the diabetic group (intergroup difference: P< 0.05). Pgly was 6.1 × 10-6 (SE 1.8 × 10-6) and 74 × 10-6 (SE 42 × 10-6) cm/s in the healthy and diabetic participants, respectively (P × 0.0001). No rebound phenomenon was observed in either group. CONCLUSIONS. The maximum reduction in MV was doubled in the healthy group compared with the diabetic group, whereas the glycerol permeability was 12 times higher in the diabetic participants. These findings confirm the paradigm of BRB breakdown in DME, but also suggest a novel procedure for the determination of retinal permeability to various agents, which is independent of the vitreous condition (ClinicalTrials.gov number, NCT00333671).

U2 - 10.1167/iovs.09-4172

DO - 10.1167/iovs.09-4172

M3 - Journal article

C2 - 20042642

SN - 0146-0404

VL - 51

SP - 2827

EP - 2834

JO - Investigative Ophthalmology & Visual Science

JF - Investigative Ophthalmology & Visual Science

IS - 6

ER -

Blood-retinal barrier glycerol permeability in diabetic macular edema and healthy eyes: estimations from macular volume changes after peroral glycerol

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