Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

M Itkin; U Heinig; O Tzfadia; A J Bhide; B Shinde; P D Cardenas; S E Bocobza; T Unger; S Malitsky; R Finkers; Y Tikunov; A Bovy; Y Chikate; P Singh; I Rogachev; J Beekwilder; A P Giri; A Aharoni

doi:10.1126/science.1240230

Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

M Itkin, U Heinig, O Tzfadia, A J Bhide, B Shinde, P D Cardenas, S E Bocobza, T Unger, S Malitsky, R Finkers, Y Tikunov, A Bovy, Y Chikate, P Singh, I Rogachev, J Beekwilder, A P Giri, A Aharoni

Section for Plant Biochemistry

272 Citations (Scopus)

Abstract

Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

Original language	English
Journal	Science
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.1240230
Publication status	Published - 2013

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Itkin, M., Heinig, U., Tzfadia, O., Bhide, A. J., Shinde, B., Cardenas, P. D., Bocobza, S. E., Unger, T., Malitsky, S., Finkers, R., Tikunov, Y., Bovy, A., Chikate, Y., Singh, P., Rogachev, I., Beekwilder, J., Giri, A. P., & Aharoni, A. (2013). Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes. Science. https://doi.org/10.1126/science.1240230

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title = "Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes",

abstract = "Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.",

author = "M Itkin and U Heinig and O Tzfadia and Bhide, {A J} and B Shinde and Cardenas, {P D} and Bocobza, {S E} and T Unger and S Malitsky and R Finkers and Y Tikunov and A Bovy and Y Chikate and P Singh and I Rogachev and J Beekwilder and Giri, {A P} and A Aharoni",

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doi = "10.1126/science.1240230",

language = "English",

journal = "Science",

issn = "0036-8075",

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TY - JOUR

T1 - Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

AU - Itkin, M

AU - Heinig, U

AU - Tzfadia, O

AU - Bhide, A J

AU - Shinde, B

AU - Cardenas, P D

AU - Bocobza, S E

AU - Unger, T

AU - Malitsky, S

AU - Finkers, R

AU - Tikunov, Y

AU - Bovy, A

AU - Chikate, Y

AU - Singh, P

AU - Rogachev, I

AU - Beekwilder, J

AU - Giri, A P

AU - Aharoni, A

PY - 2013

Y1 - 2013

N2 - Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

AB - Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

U2 - 10.1126/science.1240230

DO - 10.1126/science.1240230

M3 - Journal article

C2 - 23788733

SN - 0036-8075

JO - Science

JF - Science

ER -

Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

Abstract

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