Biomimetic Membrane Arrays on Cast Hydrogel Supports

Monique Roerdink Lander; Sania Ibragimova; Christian Rein; Jorg Vogel; Karin Stibius; Oliver Geschke; Mark Perry; Claus Helix-Nielsen

doi:10.1021/la1050699

Biomimetic Membrane Arrays on Cast Hydrogel Supports

Monique Roerdink Lander, Sania Ibragimova, Christian Rein, Jorg Vogel, Karin Stibius, Oliver Geschke, Mark Perry, Claus Helix-Nielsen

Department of Chemistry

18 Citations (Scopus)

Abstract

Lipid bilayers are intrinsically fragile and require mechanical support in technical applications based on biomimetic membranes. Tethering the lipid bilayer membranes to solid substrates, either directly through covalent or ionic substrate lipid links or indirectly on substrate-supported cushions, provides mechanical support but at the cost of small molecule transport through the membrane support sandwich. To stabilize biomimetic membranes while allowing transport through a membrane support sandwich, we have investigated the feasibility of using an ethylene tetrafluoro ethylene (ETFE)/hydrogel sandwich as the support. The sandwich is realized as a perforated surface-treated ETFE film onto which a hydrogel composite support structure is cast. We report a simple method to prepare arrays of lipid bilayer membranes with low intrinsic electrical conductance on the highly permeable, self-supporting ETFE/hydrogel sandwiches. We demonstrate how the ETFE/hydrogel sandwich support promotes rapid self-thinning of lipid bilayers suitable for hosting membrane-spanning proteins.

Original language	English
Journal	Langmuir
Volume	27
Issue number	11
Pages (from-to)	7002-7007
Number of pages	6
ISSN	0743-7463
DOIs	https://doi.org/10.1021/la1050699
Publication status	Published - 7 Jun 2011

Access to Document

10.1021/la1050699

Cite this

@article{a8ee8c313cba4822a518332115e403d9,

title = "Biomimetic Membrane Arrays on Cast Hydrogel Supports",

abstract = "Lipid bilayers are intrinsically fragile and require mechanical support in technical applications based on biomimetic membranes. Tethering the lipid bilayer membranes to solid substrates, either directly through covalent or ionic substrate lipid links or indirectly on substrate-supported cushions, provides mechanical support but at the cost of small molecule transport through the membrane support sandwich. To stabilize biomimetic membranes while allowing transport through a membrane support sandwich, we have investigated the feasibility of using an ethylene tetrafluoro ethylene (ETFE)/hydrogel sandwich as the support. The sandwich is realized as a perforated surface-treated ETFE film onto which a hydrogel composite support structure is cast. We report a simple method to prepare arrays of lipid bilayer membranes with low intrinsic electrical conductance on the highly permeable, self-supporting ETFE/hydrogel sandwiches. We demonstrate how the ETFE/hydrogel sandwich support promotes rapid self-thinning of lipid bilayers suitable for hosting membrane-spanning proteins.",

author = "Lander, {Monique Roerdink} and Sania Ibragimova and Christian Rein and Jorg Vogel and Karin Stibius and Oliver Geschke and Mark Perry and Claus Helix-Nielsen",

year = "2011",

month = jun,

day = "7",

doi = "10.1021/la1050699",

language = "English",

volume = "27",

pages = "7002--7007",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American Chemical Society",

number = "11",

}

TY - JOUR

T1 - Biomimetic Membrane Arrays on Cast Hydrogel Supports

AU - Lander, Monique Roerdink

AU - Ibragimova, Sania

AU - Rein, Christian

AU - Vogel, Jorg

AU - Stibius, Karin

AU - Geschke, Oliver

AU - Perry, Mark

AU - Helix-Nielsen, Claus

PY - 2011/6/7

Y1 - 2011/6/7

N2 - Lipid bilayers are intrinsically fragile and require mechanical support in technical applications based on biomimetic membranes. Tethering the lipid bilayer membranes to solid substrates, either directly through covalent or ionic substrate lipid links or indirectly on substrate-supported cushions, provides mechanical support but at the cost of small molecule transport through the membrane support sandwich. To stabilize biomimetic membranes while allowing transport through a membrane support sandwich, we have investigated the feasibility of using an ethylene tetrafluoro ethylene (ETFE)/hydrogel sandwich as the support. The sandwich is realized as a perforated surface-treated ETFE film onto which a hydrogel composite support structure is cast. We report a simple method to prepare arrays of lipid bilayer membranes with low intrinsic electrical conductance on the highly permeable, self-supporting ETFE/hydrogel sandwiches. We demonstrate how the ETFE/hydrogel sandwich support promotes rapid self-thinning of lipid bilayers suitable for hosting membrane-spanning proteins.

AB - Lipid bilayers are intrinsically fragile and require mechanical support in technical applications based on biomimetic membranes. Tethering the lipid bilayer membranes to solid substrates, either directly through covalent or ionic substrate lipid links or indirectly on substrate-supported cushions, provides mechanical support but at the cost of small molecule transport through the membrane support sandwich. To stabilize biomimetic membranes while allowing transport through a membrane support sandwich, we have investigated the feasibility of using an ethylene tetrafluoro ethylene (ETFE)/hydrogel sandwich as the support. The sandwich is realized as a perforated surface-treated ETFE film onto which a hydrogel composite support structure is cast. We report a simple method to prepare arrays of lipid bilayer membranes with low intrinsic electrical conductance on the highly permeable, self-supporting ETFE/hydrogel sandwiches. We demonstrate how the ETFE/hydrogel sandwich support promotes rapid self-thinning of lipid bilayers suitable for hosting membrane-spanning proteins.

U2 - 10.1021/la1050699

DO - 10.1021/la1050699

M3 - Journal article

C2 - 21526805

SN - 0743-7463

VL - 27

SP - 7002

EP - 7007

JO - Langmuir

JF - Langmuir

IS - 11

ER -