Biological Trojan horse: Antigen 43 provides specific bacterial uptake and survival in human neutrophils

Sara Fexby; Thomas Bjarnsholt; Peter Østrup Jensen; Viktoria Roos; Niels Høiby; Michael Givskov; Per Klemm

doi:10.1128/IAI.01117-06

Biological Trojan horse: Antigen 43 provides specific bacterial uptake and survival in human neutrophils

Sara Fexby, Thomas Bjarnsholt, Peter Østrup Jensen, Viktoria Roos, Niels Høiby, Michael Givskov, Per Klemm

39 Citations (Scopus)

Abstract

Escherichia coli is a versatile pathogen causing millions of infections in humans every year. This bacterium can form multicellular aggregates when it expresses a self-associating protein, antigen 43 (Ag43), on its surface. We have discovered that Ag43-expressing E. coli cells are efficiently taken up by human defense cells, polymorphonuclear neutrophils (PMNs), in an opsonin-independent manner. Surprisingly, the phagocytosed bacteria were not immediately killed but resided as tight aggregates within the PMNs. Our observations indicate that Ag43-mediated uptake and survival in PMNs constitute a mechanism to subvert one of the primary defense mechanisms of the human body.

Original language	English
Journal	Infection and Immunity
Volume	75
Issue number	1
Pages (from-to)	30-4
Number of pages	4
ISSN	0019-9567
DOIs	https://doi.org/10.1128/IAI.01117-06
Publication status	Published - 2006

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title = "Biological Trojan horse: Antigen 43 provides specific bacterial uptake and survival in human neutrophils",

abstract = "Escherichia coli is a versatile pathogen causing millions of infections in humans every year. This bacterium can form multicellular aggregates when it expresses a self-associating protein, antigen 43 (Ag43), on its surface. We have discovered that Ag43-expressing E. coli cells are efficiently taken up by human defense cells, polymorphonuclear neutrophils (PMNs), in an opsonin-independent manner. Surprisingly, the phagocytosed bacteria were not immediately killed but resided as tight aggregates within the PMNs. Our observations indicate that Ag43-mediated uptake and survival in PMNs constitute a mechanism to subvert one of the primary defense mechanisms of the human body.",

author = "Sara Fexby and Thomas Bjarnsholt and Jensen, {Peter {\O}strup} and Viktoria Roos and Niels H{\o}iby and Michael Givskov and Per Klemm",

note = "Keywords: Adhesins, Bacterial; Antigens, Bacterial; Bacterial Outer Membrane Proteins; Cells, Cultured; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Humans; Neutrophils; Phagocytosis",

year = "2006",

doi = "10.1128/IAI.01117-06",

language = "English",

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T1 - Biological Trojan horse: Antigen 43 provides specific bacterial uptake and survival in human neutrophils

AU - Fexby, Sara

AU - Bjarnsholt, Thomas

AU - Jensen, Peter Østrup

AU - Roos, Viktoria

AU - Høiby, Niels

AU - Givskov, Michael

AU - Klemm, Per

N1 - Keywords: Adhesins, Bacterial; Antigens, Bacterial; Bacterial Outer Membrane Proteins; Cells, Cultured; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Humans; Neutrophils; Phagocytosis

PY - 2006

Y1 - 2006

N2 - Escherichia coli is a versatile pathogen causing millions of infections in humans every year. This bacterium can form multicellular aggregates when it expresses a self-associating protein, antigen 43 (Ag43), on its surface. We have discovered that Ag43-expressing E. coli cells are efficiently taken up by human defense cells, polymorphonuclear neutrophils (PMNs), in an opsonin-independent manner. Surprisingly, the phagocytosed bacteria were not immediately killed but resided as tight aggregates within the PMNs. Our observations indicate that Ag43-mediated uptake and survival in PMNs constitute a mechanism to subvert one of the primary defense mechanisms of the human body.

AB - Escherichia coli is a versatile pathogen causing millions of infections in humans every year. This bacterium can form multicellular aggregates when it expresses a self-associating protein, antigen 43 (Ag43), on its surface. We have discovered that Ag43-expressing E. coli cells are efficiently taken up by human defense cells, polymorphonuclear neutrophils (PMNs), in an opsonin-independent manner. Surprisingly, the phagocytosed bacteria were not immediately killed but resided as tight aggregates within the PMNs. Our observations indicate that Ag43-mediated uptake and survival in PMNs constitute a mechanism to subvert one of the primary defense mechanisms of the human body.

U2 - 10.1128/IAI.01117-06

DO - 10.1128/IAI.01117-06

M3 - Journal article

C2 - 17030570

SN - 0019-9567

VL - 75

SP - 30

EP - 34

JO - Infection and Immunity

JF - Infection and Immunity

IS - 1

ER -

Biological Trojan horse: Antigen 43 provides specific bacterial uptake and survival in human neutrophils

Abstract

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