Bioaugmentation with Petroleum-Degrading Consortia Has a Selective Growth-Promoting Impact on Crop Plants Germinated in Diesel Oil-Contaminated Soil

TY - JOUR

T1 - Bioaugmentation with Petroleum-Degrading Consortia Has a Selective Growth-Promoting Impact on Crop Plants Germinated in Diesel Oil-Contaminated Soil

AU - Czaban, Weronika

PY - 2013/9

Y1 - 2013/9

N2 - Rhizoremediation is a complex type of greenclean-up technology that involves both plants and therhizosphere-associated microorganisms to decomposehazardous compounds. The success of the strategystrongly depends on plant tolerance towards the pollutant,as well as plant's interactions with the rhizosphericmicrobes. The microorganisms may be stimulated by thesecreted root exudates, which results in an increasedbreakdown of contaminants in the rhizosphere. The maingoal of this study was to establish a potentialrhizoremediation combination for a diesel-polluted site.Inoculation of plant roots or seeds with indigenousrhizospheric populations is a common approach in therhizoremediation. However, we introduced hydrocarbondegradingconsortia (M10, R3, and K52) that were previouslyisolated from crude oil-contaminated soil insteadof indigenous microbes. Bioaugmentation with thesepetroleum degraders was applied to screen four highbiomass crop species (Indian mustard, alfalfa, high erucicacid rapeseed, HEAR, and low erucic acid rapeseed,LEAR) for their tolerance towards diesel oil. At nopollution, a promoting effect of M10 bacteria could beobserved on germination and root elongation of all plantspecies. Moreover, M10 consortiums increased the germinationindex at 6,000 mg diesel oil per kilogram drysoil in the case of Indian mustard, alfalfa, and HEAR.The latter species was found to increment its dry weightupon bioaugmentation with M10 bacteria and all dieseloil treatments (6,000 and 24,000 mg diesel oil per kilogramdry soil). The initial results indicate HEAR and theM10 bacterial consortium as a promising plant–microbetandem for a long-term rhizoremediation process.

AB - Rhizoremediation is a complex type of greenclean-up technology that involves both plants and therhizosphere-associated microorganisms to decomposehazardous compounds. The success of the strategystrongly depends on plant tolerance towards the pollutant,as well as plant's interactions with the rhizosphericmicrobes. The microorganisms may be stimulated by thesecreted root exudates, which results in an increasedbreakdown of contaminants in the rhizosphere. The maingoal of this study was to establish a potentialrhizoremediation combination for a diesel-polluted site.Inoculation of plant roots or seeds with indigenousrhizospheric populations is a common approach in therhizoremediation. However, we introduced hydrocarbondegradingconsortia (M10, R3, and K52) that were previouslyisolated from crude oil-contaminated soil insteadof indigenous microbes. Bioaugmentation with thesepetroleum degraders was applied to screen four highbiomass crop species (Indian mustard, alfalfa, high erucicacid rapeseed, HEAR, and low erucic acid rapeseed,LEAR) for their tolerance towards diesel oil. At nopollution, a promoting effect of M10 bacteria could beobserved on germination and root elongation of all plantspecies. Moreover, M10 consortiums increased the germinationindex at 6,000 mg diesel oil per kilogram drysoil in the case of Indian mustard, alfalfa, and HEAR.The latter species was found to increment its dry weightupon bioaugmentation with M10 bacteria and all dieseloil treatments (6,000 and 24,000 mg diesel oil per kilogramdry soil). The initial results indicate HEAR and theM10 bacterial consortium as a promising plant–microbetandem for a long-term rhizoremediation process.

M3 - Journal article

VL - 224

JO - Water, Air, & Soil Polution

JF - Water, Air, & Soil Polution

IS - 9

ER -