TY - JOUR
T1 - Barley isochorismate synthase mutant is phylloquinone-deficient, but has normal basal salicylic acid level
AU - Qin, Yuan
AU - Torp, Anna Maria
AU - Glauser, Gaëtan
AU - Pedersen, Carsten
AU - Rasmussen, Søren K.
AU - Thordal-Christensen, Hans
PY - 2019/11/2
Y1 - 2019/11/2
N2 - Salicylic acid (SA) is an important signaling hormone in plant immunity. It can be synthesized by either the phenylpropanoid pathway or the isochorismate pathway, but mutant studies of this have been scarce in other species than Arabidopsis. Here we identified a mutation that introduced a stop-codon early in the barley gene for isochorismate synthase (ICS). We found that homozygous ics plants wilted if not sprayed with 1,4-dihydroxy-2-naphthoic acid, a precursor of phylloquinone, also synthesized via the isochorismate pathway. Interestingly, ics had unchanged SA, suggesting that the basal level of SA is synthesized via the phenylpropanoid pathway. Previous studies have failed seeing increased SA levels in barley after attack by the powdery mildew fungus, Blumeria graminis f.sp. hordei (Bgh), and indeed, we saw no changes in the interaction of ics with this fungus. Overall, we hope this mutant will be useful for other studies of SA in barley.
AB - Salicylic acid (SA) is an important signaling hormone in plant immunity. It can be synthesized by either the phenylpropanoid pathway or the isochorismate pathway, but mutant studies of this have been scarce in other species than Arabidopsis. Here we identified a mutation that introduced a stop-codon early in the barley gene for isochorismate synthase (ICS). We found that homozygous ics plants wilted if not sprayed with 1,4-dihydroxy-2-naphthoic acid, a precursor of phylloquinone, also synthesized via the isochorismate pathway. Interestingly, ics had unchanged SA, suggesting that the basal level of SA is synthesized via the phenylpropanoid pathway. Previous studies have failed seeing increased SA levels in barley after attack by the powdery mildew fungus, Blumeria graminis f.sp. hordei (Bgh), and indeed, we saw no changes in the interaction of ics with this fungus. Overall, we hope this mutant will be useful for other studies of SA in barley.
KW - barley
KW - isochorismate synthase
KW - phylloquinone
KW - Salicylic acid
UR - http://www.scopus.com/inward/record.url?scp=85073307380&partnerID=8YFLogxK
U2 - 10.1080/15592324.2019.1671122
DO - 10.1080/15592324.2019.1671122
M3 - Journal article
C2 - 31559895
AN - SCOPUS:85073307380
SN - 1559-2316
VL - 14
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
IS - 11
M1 - 1671122
ER -