Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark

Maria Wessman; Kristina Thorsteinsson; Jørgen S. Jensen; Merete Storgaard; Frederikke F. Rönsholt; Isik S. Johansen; Gitte Pedersen; Lars Nørregård Nielsen; Jesper Bonde; Terese L. Katzenstein; Nina Weis; Anne Mette Lebech

doi:10.1186/s12879-017-2477-7

Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark

Maria Wessman^*, Kristina Thorsteinsson, Jørgen S. Jensen, Merete Storgaard, Frederikke F. Rönsholt, Isik S. Johansen, Gitte Pedersen, Lars Nørregård Nielsen, Jesper Bonde, Terese L. Katzenstein, Nina Weis, Anne Mette Lebech

^*Corresponding author for this work

Department of Clinical Medicine

6 Citations (Scopus)

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Abstract

Background: Bacterial vaginosis (BV) has been found to be associated with HIV acquisition and transmission. This is suggested to be due to higher HIV RNA levels in cervicovaginal fluids in women living with HIV (WLWH) with BV, as bacteria associated with BV may induce viral replication and shedding in the genital tract despite undetectable HIV RNA plasma viral load. We examined the prevalence and diagnostic predictors of BV and HIV-1 RNA vaginal shedding in women living with HIV (WLWH) in Denmark, taking into account the presence of human papillomavirus (HPV) and herpes viridae. Methods: WLWH between 18-51 years were recruited from six Departments of Infectious Diseases in Denmark during enrolment in the SHADE cohort; a prospective cohort study of WLWH attending regular outpatient care. BV was diagnosed by microscopy of vaginal swabs and PCR was used for detection of BV-associated bacteria, HPV, herpes viridae, and vaginal HIV viral load. Results: Median age of the 150 included women was 41 years; ethnicity was predominantly White (35%) or Black (47%). The majority (96%) was on ART and had undetectable (85%) plasma HIV RNA (<40 copies/mL). BV was diagnosed in 32%. Overall, 11% had detectable vaginal HIV RNA. Both before and after adjustment for BV, age, ethnicity, plasma HIV RNA, CD4 cell count, herpes viridae and HPV, we found no significant predictors of HIV RNA vaginal shedding. Conclusion: In well-treated WLWH, BV, herpes viridae or HPV do not predict vaginal HIV RNA shedding. This implies that HIV shedding does not seem to be increased by BV.

Original language	English
Article number	376
Journal	BMC Infectious Diseases
Volume	17
Number of pages	10
ISSN	1471-2334
DOIs	https://doi.org/10.1186/s12879-017-2477-7
Publication status	Published - 31 May 2017

Keywords

Bacterial vaginosis
Herpes viridae
HIV RNA vaginal shedding
Human papilloma virus
Women living with HIV

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Wessman, M., Thorsteinsson, K., Jensen, J. S., Storgaard, M., Rönsholt, F. F., Johansen, I. S., Pedersen, G., Nørregård Nielsen, L., Bonde, J., Katzenstein, T. L., Weis, N., & Lebech, A. M. (2017). Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark. BMC Infectious Diseases, 17, Article 376. https://doi.org/10.1186/s12879-017-2477-7

Wessman, M, Thorsteinsson, K, Jensen, JS, Storgaard, M, Rönsholt, FF, Johansen, IS, Pedersen, G, Nørregård Nielsen, L, Bonde, J, Katzenstein, TL, Weis, N & Lebech, AM 2017, 'Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark', BMC Infectious Diseases, vol. 17, 376. https://doi.org/10.1186/s12879-017-2477-7

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title = "Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark",

abstract = "Background: Bacterial vaginosis (BV) has been found to be associated with HIV acquisition and transmission. This is suggested to be due to higher HIV RNA levels in cervicovaginal fluids in women living with HIV (WLWH) with BV, as bacteria associated with BV may induce viral replication and shedding in the genital tract despite undetectable HIV RNA plasma viral load. We examined the prevalence and diagnostic predictors of BV and HIV-1 RNA vaginal shedding in women living with HIV (WLWH) in Denmark, taking into account the presence of human papillomavirus (HPV) and herpes viridae. Methods: WLWH between 18-51 years were recruited from six Departments of Infectious Diseases in Denmark during enrolment in the SHADE cohort; a prospective cohort study of WLWH attending regular outpatient care. BV was diagnosed by microscopy of vaginal swabs and PCR was used for detection of BV-associated bacteria, HPV, herpes viridae, and vaginal HIV viral load. Results: Median age of the 150 included women was 41 years; ethnicity was predominantly White (35%) or Black (47%). The majority (96%) was on ART and had undetectable (85%) plasma HIV RNA (<40 copies/mL). BV was diagnosed in 32%. Overall, 11% had detectable vaginal HIV RNA. Both before and after adjustment for BV, age, ethnicity, plasma HIV RNA, CD4 cell count, herpes viridae and HPV, we found no significant predictors of HIV RNA vaginal shedding. Conclusion: In well-treated WLWH, BV, herpes viridae or HPV do not predict vaginal HIV RNA shedding. This implies that HIV shedding does not seem to be increased by BV.",

keywords = "Bacterial vaginosis, Herpes viridae, HIV RNA vaginal shedding, Human papilloma virus, Women living with HIV",

author = "Maria Wessman and Kristina Thorsteinsson and Jensen, {J{\o}rgen S.} and Merete Storgaard and R{\"o}nsholt, {Frederikke F.} and Johansen, {Isik S.} and Gitte Pedersen and {N{\o}rreg{\aa}rd Nielsen}, Lars and Jesper Bonde and Katzenstein, {Terese L.} and Nina Weis and Lebech, {Anne Mette}",

year = "2017",

month = may,

day = "31",

doi = "10.1186/s12879-017-2477-7",

language = "English",

volume = "17",

journal = "BMC Infectious Diseases",

issn = "1471-2334",

publisher = "BioMed Central Ltd.",

}

TY - JOUR

T1 - Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark

AU - Wessman, Maria

AU - Thorsteinsson, Kristina

AU - Jensen, Jørgen S.

AU - Storgaard, Merete

AU - Rönsholt, Frederikke F.

AU - Johansen, Isik S.

AU - Pedersen, Gitte

AU - Nørregård Nielsen, Lars

AU - Bonde, Jesper

AU - Katzenstein, Terese L.

AU - Weis, Nina

AU - Lebech, Anne Mette

PY - 2017/5/31

Y1 - 2017/5/31

N2 - Background: Bacterial vaginosis (BV) has been found to be associated with HIV acquisition and transmission. This is suggested to be due to higher HIV RNA levels in cervicovaginal fluids in women living with HIV (WLWH) with BV, as bacteria associated with BV may induce viral replication and shedding in the genital tract despite undetectable HIV RNA plasma viral load. We examined the prevalence and diagnostic predictors of BV and HIV-1 RNA vaginal shedding in women living with HIV (WLWH) in Denmark, taking into account the presence of human papillomavirus (HPV) and herpes viridae. Methods: WLWH between 18-51 years were recruited from six Departments of Infectious Diseases in Denmark during enrolment in the SHADE cohort; a prospective cohort study of WLWH attending regular outpatient care. BV was diagnosed by microscopy of vaginal swabs and PCR was used for detection of BV-associated bacteria, HPV, herpes viridae, and vaginal HIV viral load. Results: Median age of the 150 included women was 41 years; ethnicity was predominantly White (35%) or Black (47%). The majority (96%) was on ART and had undetectable (85%) plasma HIV RNA (<40 copies/mL). BV was diagnosed in 32%. Overall, 11% had detectable vaginal HIV RNA. Both before and after adjustment for BV, age, ethnicity, plasma HIV RNA, CD4 cell count, herpes viridae and HPV, we found no significant predictors of HIV RNA vaginal shedding. Conclusion: In well-treated WLWH, BV, herpes viridae or HPV do not predict vaginal HIV RNA shedding. This implies that HIV shedding does not seem to be increased by BV.

AB - Background: Bacterial vaginosis (BV) has been found to be associated with HIV acquisition and transmission. This is suggested to be due to higher HIV RNA levels in cervicovaginal fluids in women living with HIV (WLWH) with BV, as bacteria associated with BV may induce viral replication and shedding in the genital tract despite undetectable HIV RNA plasma viral load. We examined the prevalence and diagnostic predictors of BV and HIV-1 RNA vaginal shedding in women living with HIV (WLWH) in Denmark, taking into account the presence of human papillomavirus (HPV) and herpes viridae. Methods: WLWH between 18-51 years were recruited from six Departments of Infectious Diseases in Denmark during enrolment in the SHADE cohort; a prospective cohort study of WLWH attending regular outpatient care. BV was diagnosed by microscopy of vaginal swabs and PCR was used for detection of BV-associated bacteria, HPV, herpes viridae, and vaginal HIV viral load. Results: Median age of the 150 included women was 41 years; ethnicity was predominantly White (35%) or Black (47%). The majority (96%) was on ART and had undetectable (85%) plasma HIV RNA (<40 copies/mL). BV was diagnosed in 32%. Overall, 11% had detectable vaginal HIV RNA. Both before and after adjustment for BV, age, ethnicity, plasma HIV RNA, CD4 cell count, herpes viridae and HPV, we found no significant predictors of HIV RNA vaginal shedding. Conclusion: In well-treated WLWH, BV, herpes viridae or HPV do not predict vaginal HIV RNA shedding. This implies that HIV shedding does not seem to be increased by BV.

KW - Bacterial vaginosis

KW - Herpes viridae

KW - HIV RNA vaginal shedding

KW - Human papilloma virus

KW - Women living with HIV

U2 - 10.1186/s12879-017-2477-7

DO - 10.1186/s12879-017-2477-7

M3 - Journal article

C2 - 28569142

AN - SCOPUS:85020039505

SN - 1471-2334

VL - 17

JO - BMC Infectious Diseases

JF - BMC Infectious Diseases

M1 - 376

ER -

Bacterial vaginosis, human papilloma virus and herpes viridae do not predict vaginal HIV RNA shedding in women living with HIV in Denmark

Abstract

Keywords

UN SDGs

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