Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties

Paul Farrow; Konstantin Khodosevich; Yechiam Sapir; Anton Schulmann; Muhammad Aslam; Yael Stern-Bach; Hannah Monyer; Jakob von Engelhardt

doi:10.7554/elife.09693

Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties

Paul Farrow, Konstantin Khodosevich, Yechiam Sapir, Anton Schulmann, Muhammad Aslam, Yael Stern-Bach, Hannah Monyer, Jakob von Engelhardt

33 Citations (Scopus)

Abstract

AMPA receptor (AMPAR) function is modulated by auxiliary subunits. Here, we report on three AMPAR interacting proteins-namely CKAMP39, CKAMP52 and CKAMP59-that, together with the previously characterized CKAMP44, constitute a novel family of auxiliary subunits distinct from other families of AMPAR interacting proteins. The new members of the CKAMP family display distinct regional and developmental expression profiles in the mouse brain. Notably, despite their structural similarities they exert diverse modulation on AMPAR gating by influencing deactivation, desensitization and recovery from desensitization, as well as glutamate and cyclothiazide potency to AMPARs. This study indicates that AMPAR function is very precisely controlled by the cell-type specific expression of the CKAMP family members.

Original language	English
Journal	eLife
Volume	4
Pages (from-to)	e09693
ISSN	2050-084X
DOIs	https://doi.org/10.7554/elife.09693
Publication status	Published - 1 Dec 2015
Externally published	Yes

Keywords

Animals
Benzothiadiazines
Brain
Carrier Proteins
Gene Expression Regulation, Developmental
Glutamic Acid
Humans
Membrane Proteins
Mice
Protein Binding
Receptors, AMPA
Sequence Analysis, DNA
Journal Article
Research Support, Non-U.S. Gov't

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title = "Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties",

abstract = "AMPA receptor (AMPAR) function is modulated by auxiliary subunits. Here, we report on three AMPAR interacting proteins-namely CKAMP39, CKAMP52 and CKAMP59-that, together with the previously characterized CKAMP44, constitute a novel family of auxiliary subunits distinct from other families of AMPAR interacting proteins. The new members of the CKAMP family display distinct regional and developmental expression profiles in the mouse brain. Notably, despite their structural similarities they exert diverse modulation on AMPAR gating by influencing deactivation, desensitization and recovery from desensitization, as well as glutamate and cyclothiazide potency to AMPARs. This study indicates that AMPAR function is very precisely controlled by the cell-type specific expression of the CKAMP family members.",

keywords = "Animals, Benzothiadiazines, Brain, Carrier Proteins, Gene Expression Regulation, Developmental, Glutamic Acid, Humans, Membrane Proteins, Mice, Protein Binding, Receptors, AMPA, Sequence Analysis, DNA, Journal Article, Research Support, Non-U.S. Gov't",

author = "Paul Farrow and Konstantin Khodosevich and Yechiam Sapir and Anton Schulmann and Muhammad Aslam and Yael Stern-Bach and Hannah Monyer and {von Engelhardt}, Jakob",

year = "2015",

month = dec,

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doi = "10.7554/elife.09693",

language = "English",

volume = "4",

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journal = "eLife",

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TY - JOUR

T1 - Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties

AU - Farrow, Paul

AU - Khodosevich, Konstantin

AU - Sapir, Yechiam

AU - Schulmann, Anton

AU - Aslam, Muhammad

AU - Stern-Bach, Yael

AU - Monyer, Hannah

AU - von Engelhardt, Jakob

PY - 2015/12/1

Y1 - 2015/12/1

N2 - AMPA receptor (AMPAR) function is modulated by auxiliary subunits. Here, we report on three AMPAR interacting proteins-namely CKAMP39, CKAMP52 and CKAMP59-that, together with the previously characterized CKAMP44, constitute a novel family of auxiliary subunits distinct from other families of AMPAR interacting proteins. The new members of the CKAMP family display distinct regional and developmental expression profiles in the mouse brain. Notably, despite their structural similarities they exert diverse modulation on AMPAR gating by influencing deactivation, desensitization and recovery from desensitization, as well as glutamate and cyclothiazide potency to AMPARs. This study indicates that AMPAR function is very precisely controlled by the cell-type specific expression of the CKAMP family members.

AB - AMPA receptor (AMPAR) function is modulated by auxiliary subunits. Here, we report on three AMPAR interacting proteins-namely CKAMP39, CKAMP52 and CKAMP59-that, together with the previously characterized CKAMP44, constitute a novel family of auxiliary subunits distinct from other families of AMPAR interacting proteins. The new members of the CKAMP family display distinct regional and developmental expression profiles in the mouse brain. Notably, despite their structural similarities they exert diverse modulation on AMPAR gating by influencing deactivation, desensitization and recovery from desensitization, as well as glutamate and cyclothiazide potency to AMPARs. This study indicates that AMPAR function is very precisely controlled by the cell-type specific expression of the CKAMP family members.

KW - Animals

KW - Benzothiadiazines

KW - Brain

KW - Carrier Proteins

KW - Gene Expression Regulation, Developmental

KW - Glutamic Acid

KW - Humans

KW - Membrane Proteins

KW - Mice

KW - Protein Binding

KW - Receptors, AMPA

KW - Sequence Analysis, DNA

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.7554/elife.09693

DO - 10.7554/elife.09693

M3 - Journal article

C2 - 26623514

SN - 2050-084X

VL - 4

SP - e09693

JO - eLife

JF - eLife

ER -

Auxiliary subunits of the CKAMP family differentially modulate AMPA receptor properties

Abstract

Keywords

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