Assumed non-persistent environmental chemicals in human adipose tissue; matrix stability and correlation with levels measured in urine and serum

F Artacho-Cordón; J P Arrebola; O Nielsen; P Hernández; N E Skakkebaek; M F Fernández; A M Andersson; N Olea; H Frederiksen

doi:10.1016/j.envres.2017.03.030

Assumed non-persistent environmental chemicals in human adipose tissue; matrix stability and correlation with levels measured in urine and serum

F Artacho-Cordón, J P Arrebola, O Nielsen, P Hernández, N E Skakkebaek, M F Fernández, A M Andersson, N Olea, H Frederiksen

Department of Clinical Medicine

30 Citations (Scopus)

Abstract

The aim of this study was to (1) optimize a method for the measurement of parabens and phenols in adipose tissue, (2) evaluate the stability of chemical residues in adipose tissue samples, and (3) study correlations of these compounds in urine, serum, and adipose tissue. Samples were obtained from adults undergoing trauma surgery. Nine phenols and seven parabens were determined by isotope diluted TurboFlow-LC-MS/MS. The analytical method showed good accuracy and precision. Limits of detection (LOD) for parabens and phenols ranged from 0.05 to 1.83ng/g tissue. Good recovery rates were found, even when biological samples remained defrosted up to 24h. Benzophenone-3 (BP-3; range of values: <LOD-1.48ng/g tissue) and methylparaben (MeP; <LOD-1.78ng/g tissue) were detected in >70% of adipose tissue samples, while bisphenol-A (BPA; <LOD-3.28ng/g tissue) and 2-phenylphenol (2-PP; <LOD-0.78ng/g tissue) were detected in >40% of adipose tissue samples. In general, levels were similar between adipose tissue and serum, while a correlation between adipose tissue and urine was only found for BP-3. In conclusion, adipose tissue samples in this study were found to contain environmental chemicals considered to be non-persistent, whose levels were weakly or not at all correlated with the urine burden. Therefore, adipose tissue may potentially provide additional information to that obtained from other biological matrices. Further investigations are warranted to explore whether adipose tissue might be a suitable matrix for assessment of the consequences for human health of mid/long-term exposure to these chemicals.

Original language	English
Journal	Environmental Research
Volume	156
Pages (from-to)	120-127
Number of pages	8
ISSN	0013-9351
DOIs	https://doi.org/10.1016/j.envres.2017.03.030
Publication status	Published - Jul 2017

Keywords

Adipose Tissue/chemistry
Adolescent
Adult
Aged
Chromatography, Liquid
Endocrine Disruptors/blood
Environmental Exposure
Environmental Monitoring/methods
Environmental Pollutants/blood
Female
Humans
Male
Middle Aged
Parabens/metabolism
Phenols/blood
Pilot Projects
Tandem Mass Spectrometry
Young Adult

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.envres.2017.03.030

Cite this

Artacho-Cordón, F., Arrebola, J. P., Nielsen, O., Hernández, P., Skakkebaek, N. E., Fernández, M. F., Andersson, A. M., Olea, N., & Frederiksen, H. (2017). Assumed non-persistent environmental chemicals in human adipose tissue; matrix stability and correlation with levels measured in urine and serum. Environmental Research, 156, 120-127. https://doi.org/10.1016/j.envres.2017.03.030

Artacho-Cordón, F, Arrebola, JP, Nielsen, O, Hernández, P, Skakkebaek, NE, Fernández, MF, Andersson, AM, Olea, N & Frederiksen, H 2017, 'Assumed non-persistent environmental chemicals in human adipose tissue; matrix stability and correlation with levels measured in urine and serum', Environmental Research, vol. 156, pp. 120-127. https://doi.org/10.1016/j.envres.2017.03.030

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title = "Assumed non-persistent environmental chemicals in human adipose tissue; matrix stability and correlation with levels measured in urine and serum",

abstract = "The aim of this study was to (1) optimize a method for the measurement of parabens and phenols in adipose tissue, (2) evaluate the stability of chemical residues in adipose tissue samples, and (3) study correlations of these compounds in urine, serum, and adipose tissue. Samples were obtained from adults undergoing trauma surgery. Nine phenols and seven parabens were determined by isotope diluted TurboFlow-LC-MS/MS. The analytical method showed good accuracy and precision. Limits of detection (LOD) for parabens and phenols ranged from 0.05 to 1.83ng/g tissue. Good recovery rates were found, even when biological samples remained defrosted up to 24h. Benzophenone-3 (BP-3; range of values: 70% of adipose tissue samples, while bisphenol-A (BPA; 40% of adipose tissue samples. In general, levels were similar between adipose tissue and serum, while a correlation between adipose tissue and urine was only found for BP-3. In conclusion, adipose tissue samples in this study were found to contain environmental chemicals considered to be non-persistent, whose levels were weakly or not at all correlated with the urine burden. Therefore, adipose tissue may potentially provide additional information to that obtained from other biological matrices. Further investigations are warranted to explore whether adipose tissue might be a suitable matrix for assessment of the consequences for human health of mid/long-term exposure to these chemicals.",

keywords = "Adipose Tissue/chemistry, Adolescent, Adult, Aged, Chromatography, Liquid, Endocrine Disruptors/blood, Environmental Exposure, Environmental Monitoring/methods, Environmental Pollutants/blood, Female, Humans, Male, Middle Aged, Parabens/metabolism, Phenols/blood, Pilot Projects, Tandem Mass Spectrometry, Young Adult",

author = "F Artacho-Cord{\'o}n and Arrebola, {J P} and O Nielsen and P Hern{\'a}ndez and Skakkebaek, {N E} and Fern{\'a}ndez, {M F} and Andersson, {A M} and N Olea and H Frederiksen",

year = "2017",

month = jul,

doi = "10.1016/j.envres.2017.03.030",

language = "English",

volume = "156",

pages = "120--127",

journal = "Environmental Research",

issn = "0013-9351",

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T1 - Assumed non-persistent environmental chemicals in human adipose tissue; matrix stability and correlation with levels measured in urine and serum

AU - Artacho-Cordón, F

AU - Arrebola, J P

AU - Nielsen, O

AU - Hernández, P

AU - Skakkebaek, N E

AU - Fernández, M F

AU - Andersson, A M

AU - Olea, N

AU - Frederiksen, H

PY - 2017/7

Y1 - 2017/7

N2 - The aim of this study was to (1) optimize a method for the measurement of parabens and phenols in adipose tissue, (2) evaluate the stability of chemical residues in adipose tissue samples, and (3) study correlations of these compounds in urine, serum, and adipose tissue. Samples were obtained from adults undergoing trauma surgery. Nine phenols and seven parabens were determined by isotope diluted TurboFlow-LC-MS/MS. The analytical method showed good accuracy and precision. Limits of detection (LOD) for parabens and phenols ranged from 0.05 to 1.83ng/g tissue. Good recovery rates were found, even when biological samples remained defrosted up to 24h. Benzophenone-3 (BP-3; range of values: 70% of adipose tissue samples, while bisphenol-A (BPA; 40% of adipose tissue samples. In general, levels were similar between adipose tissue and serum, while a correlation between adipose tissue and urine was only found for BP-3. In conclusion, adipose tissue samples in this study were found to contain environmental chemicals considered to be non-persistent, whose levels were weakly or not at all correlated with the urine burden. Therefore, adipose tissue may potentially provide additional information to that obtained from other biological matrices. Further investigations are warranted to explore whether adipose tissue might be a suitable matrix for assessment of the consequences for human health of mid/long-term exposure to these chemicals.

AB - The aim of this study was to (1) optimize a method for the measurement of parabens and phenols in adipose tissue, (2) evaluate the stability of chemical residues in adipose tissue samples, and (3) study correlations of these compounds in urine, serum, and adipose tissue. Samples were obtained from adults undergoing trauma surgery. Nine phenols and seven parabens were determined by isotope diluted TurboFlow-LC-MS/MS. The analytical method showed good accuracy and precision. Limits of detection (LOD) for parabens and phenols ranged from 0.05 to 1.83ng/g tissue. Good recovery rates were found, even when biological samples remained defrosted up to 24h. Benzophenone-3 (BP-3; range of values: 70% of adipose tissue samples, while bisphenol-A (BPA; 40% of adipose tissue samples. In general, levels were similar between adipose tissue and serum, while a correlation between adipose tissue and urine was only found for BP-3. In conclusion, adipose tissue samples in this study were found to contain environmental chemicals considered to be non-persistent, whose levels were weakly or not at all correlated with the urine burden. Therefore, adipose tissue may potentially provide additional information to that obtained from other biological matrices. Further investigations are warranted to explore whether adipose tissue might be a suitable matrix for assessment of the consequences for human health of mid/long-term exposure to these chemicals.

KW - Adipose Tissue/chemistry

KW - Adolescent

KW - Adult

KW - Aged

KW - Chromatography, Liquid

KW - Endocrine Disruptors/blood

KW - Environmental Exposure

KW - Environmental Monitoring/methods

KW - Environmental Pollutants/blood

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Parabens/metabolism

KW - Phenols/blood

KW - Pilot Projects

KW - Tandem Mass Spectrometry

KW - Young Adult

U2 - 10.1016/j.envres.2017.03.030

DO - 10.1016/j.envres.2017.03.030

M3 - Journal article

C2 - 28342347

SN - 0013-9351

VL - 156

SP - 120

EP - 127

JO - Environmental Research

JF - Environmental Research

ER -

Assumed non-persistent environmental chemicals in human adipose tissue; matrix stability and correlation with levels measured in urine and serum

Abstract

Keywords

UN SDGs

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