Associations between xerostomia, histopathological alterations, and autonomic innervation of labial salivary glands in men in late midlife

Christiane Elisabeth Sørensen; Jytte Overgaard Larsen; Jesper Reibel; Martin Lauritzen; Erik Lykke Mortensen; Merete Osler; Anne Marie Lynge Pedersen

doi:10.1016/j.exger.2014.06.004

Associations between xerostomia, histopathological alterations, and autonomic innervation of labial salivary glands in men in late midlife

Christiane Elisabeth Sørensen, Jytte Overgaard Larsen, Jesper Reibel, Martin Lauritzen, Erik Lykke Mortensen, Merete Osler, Anne Marie Lynge Pedersen

9 Citations (Scopus)

Abstract

OBJECTIVE: One aim of the present study was to investigate whether symptoms of oral dryness (xerostomia) during daytime, assessed in a study group of middle-aged male positive and negative outliers in cognition scores, were associated with age-related degenerative changes in human labial salivary glands and with quantitative measures of the glandular autonomic innervation. Another aim was to study the relation between the autonomic innervation and loss of secretory acinar cells in these glands.

METHODS: Labial salivary gland biopsies were taken from the lower lip from 190 men, born in 1953 and members of the Danish Metropolit birth cohort, who were examined for age-related changes in cognitive function and dental health as part of the Copenhagen University Center for Healthy Aging clinical neuroscience project. The glands were routinely processed and semi-quantitatively analyzed for inflammation, acinar atrophy, fibrosis, and adipocyte infiltration. Sections of labial salivary gland tissue were stained with the panneuronal marker PGP 9.5. In a subsample of 51 participants, the autonomic innervation of the glands was analyzed quantitatively by use of stereology.

RESULTS: Labial salivary gland tissue samples from 33% of all participants displayed moderate to severe acinar atrophy and fibrosis (31%). Xerostomia was not significantly associated with structural changes of labial salivary glands, but in the subsample it was inversely related to the total nerve length in the glandular connective tissue. Acinar atrophy and fibrosis were negatively correlated with the parenchymal innervation and positively related to diffuse inflammation.

CONCLUSIONS: The results from the present study indicate that aspects of the autonomic innervation of labial salivary glands may play a role in the occurrence of xerostomia which in the present study group was not significantly associated with degenerative changes in these glands. The findings further indicate that the integrity of labial salivary gland acini is related to the parenchymal autonomic innervation, whereas inflammatory processes may compromise it by alternative mechanisms.

Original language	English
Journal	Experimental Gerontology
Volume	57
Pages (from-to)	211-217
Number of pages	8
ISSN	0531-5565
DOIs	https://doi.org/10.1016/j.exger.2014.06.004
Publication status	Published - Sept 2014

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10.1016/j.exger.2014.06.004

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Associations between xerostomia, histopathological alterations, and autonomic innervation of labial salivary glands in men in late midlife. / Sørensen, Christiane Elisabeth; Larsen, Jytte Overgaard; Reibel, Jesper et al.

In: Experimental Gerontology, Vol. 57, 09.2014, p. 211-217.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{c3fc8360f4b948c9b1b8608248f73c9c,

title = "Associations between xerostomia, histopathological alterations, and autonomic innervation of labial salivary glands in men in late midlife",

abstract = "OBJECTIVE: One aim of the present study was to investigate whether symptoms of oral dryness (xerostomia) during daytime, assessed in a study group of middle-aged male positive and negative outliers in cognition scores, were associated with age-related degenerative changes in human labial salivary glands and with quantitative measures of the glandular autonomic innervation. Another aim was to study the relation between the autonomic innervation and loss of secretory acinar cells in these glands.METHODS: Labial salivary gland biopsies were taken from the lower lip from 190 men, born in 1953 and members of the Danish Metropolit birth cohort, who were examined for age-related changes in cognitive function and dental health as part of the Copenhagen University Center for Healthy Aging clinical neuroscience project. The glands were routinely processed and semi-quantitatively analyzed for inflammation, acinar atrophy, fibrosis, and adipocyte infiltration. Sections of labial salivary gland tissue were stained with the panneuronal marker PGP 9.5. In a subsample of 51 participants, the autonomic innervation of the glands was analyzed quantitatively by use of stereology.RESULTS: Labial salivary gland tissue samples from 33% of all participants displayed moderate to severe acinar atrophy and fibrosis (31%). Xerostomia was not significantly associated with structural changes of labial salivary glands, but in the subsample it was inversely related to the total nerve length in the glandular connective tissue. Acinar atrophy and fibrosis were negatively correlated with the parenchymal innervation and positively related to diffuse inflammation.CONCLUSIONS: The results from the present study indicate that aspects of the autonomic innervation of labial salivary glands may play a role in the occurrence of xerostomia which in the present study group was not significantly associated with degenerative changes in these glands. The findings further indicate that the integrity of labial salivary gland acini is related to the parenchymal autonomic innervation, whereas inflammatory processes may compromise it by alternative mechanisms.",

author = "S{\o}rensen, {Christiane Elisabeth} and Larsen, {Jytte Overgaard} and Jesper Reibel and Martin Lauritzen and Mortensen, {Erik Lykke} and Merete Osler and Pedersen, {Anne Marie Lynge}",

note = "Copyright {\textcopyright} 2014. Published by Elsevier Inc.",

year = "2014",

month = sep,

doi = "10.1016/j.exger.2014.06.004",

language = "English",

volume = "57",

pages = "211--217",

journal = "Experimental Gerontology",

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TY - JOUR

T1 - Associations between xerostomia, histopathological alterations, and autonomic innervation of labial salivary glands in men in late midlife

AU - Sørensen, Christiane Elisabeth

AU - Larsen, Jytte Overgaard

AU - Reibel, Jesper

AU - Lauritzen, Martin

AU - Mortensen, Erik Lykke

AU - Osler, Merete

AU - Pedersen, Anne Marie Lynge

PY - 2014/9

Y1 - 2014/9

N2 - OBJECTIVE: One aim of the present study was to investigate whether symptoms of oral dryness (xerostomia) during daytime, assessed in a study group of middle-aged male positive and negative outliers in cognition scores, were associated with age-related degenerative changes in human labial salivary glands and with quantitative measures of the glandular autonomic innervation. Another aim was to study the relation between the autonomic innervation and loss of secretory acinar cells in these glands.METHODS: Labial salivary gland biopsies were taken from the lower lip from 190 men, born in 1953 and members of the Danish Metropolit birth cohort, who were examined for age-related changes in cognitive function and dental health as part of the Copenhagen University Center for Healthy Aging clinical neuroscience project. The glands were routinely processed and semi-quantitatively analyzed for inflammation, acinar atrophy, fibrosis, and adipocyte infiltration. Sections of labial salivary gland tissue were stained with the panneuronal marker PGP 9.5. In a subsample of 51 participants, the autonomic innervation of the glands was analyzed quantitatively by use of stereology.RESULTS: Labial salivary gland tissue samples from 33% of all participants displayed moderate to severe acinar atrophy and fibrosis (31%). Xerostomia was not significantly associated with structural changes of labial salivary glands, but in the subsample it was inversely related to the total nerve length in the glandular connective tissue. Acinar atrophy and fibrosis were negatively correlated with the parenchymal innervation and positively related to diffuse inflammation.CONCLUSIONS: The results from the present study indicate that aspects of the autonomic innervation of labial salivary glands may play a role in the occurrence of xerostomia which in the present study group was not significantly associated with degenerative changes in these glands. The findings further indicate that the integrity of labial salivary gland acini is related to the parenchymal autonomic innervation, whereas inflammatory processes may compromise it by alternative mechanisms.

AB - OBJECTIVE: One aim of the present study was to investigate whether symptoms of oral dryness (xerostomia) during daytime, assessed in a study group of middle-aged male positive and negative outliers in cognition scores, were associated with age-related degenerative changes in human labial salivary glands and with quantitative measures of the glandular autonomic innervation. Another aim was to study the relation between the autonomic innervation and loss of secretory acinar cells in these glands.METHODS: Labial salivary gland biopsies were taken from the lower lip from 190 men, born in 1953 and members of the Danish Metropolit birth cohort, who were examined for age-related changes in cognitive function and dental health as part of the Copenhagen University Center for Healthy Aging clinical neuroscience project. The glands were routinely processed and semi-quantitatively analyzed for inflammation, acinar atrophy, fibrosis, and adipocyte infiltration. Sections of labial salivary gland tissue were stained with the panneuronal marker PGP 9.5. In a subsample of 51 participants, the autonomic innervation of the glands was analyzed quantitatively by use of stereology.RESULTS: Labial salivary gland tissue samples from 33% of all participants displayed moderate to severe acinar atrophy and fibrosis (31%). Xerostomia was not significantly associated with structural changes of labial salivary glands, but in the subsample it was inversely related to the total nerve length in the glandular connective tissue. Acinar atrophy and fibrosis were negatively correlated with the parenchymal innervation and positively related to diffuse inflammation.CONCLUSIONS: The results from the present study indicate that aspects of the autonomic innervation of labial salivary glands may play a role in the occurrence of xerostomia which in the present study group was not significantly associated with degenerative changes in these glands. The findings further indicate that the integrity of labial salivary gland acini is related to the parenchymal autonomic innervation, whereas inflammatory processes may compromise it by alternative mechanisms.

U2 - 10.1016/j.exger.2014.06.004

DO - 10.1016/j.exger.2014.06.004

M3 - Journal article

C2 - 24905142

SN - 0531-5565

VL - 57

SP - 211

EP - 217

JO - Experimental Gerontology

JF - Experimental Gerontology

ER -

Associations between xerostomia, histopathological alterations, and autonomic innervation of labial salivary glands in men in late midlife

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