Assessment of the biological variation of plasma tissue inhibitor of metalloproteinases-1

C.B. Frederiksen; Anne Fog Lomholt; Tine Lottenburger; G.J. Davis; B.L. Dowell; M.A. Blankenstein; Ib Jarle Christensen; Nils Brunner; H.J. Nielsen; Camilla Frederiksen; A F Lomholt; T Lottenburger; G J Davis; B L Dowell; M A Blankenstein; Ib Jarle Christensen; N Brunner; Hans Jørgen Nielsen

Assessment of the biological variation of plasma tissue inhibitor of metalloproteinases-1

C.B. Frederiksen, Anne Fog Lomholt, Tine Lottenburger, G.J. Davis, B.L. Dowell, M.A. Blankenstein, Ib Jarle Christensen, Nils Brunner, H.J. Nielsen, Camilla Frederiksen, A F Lomholt, T Lottenburger, G J Davis, B L Dowell, M A Blankenstein, Ib Jarle Christensen, N Brunner, Hans Jørgen Nielsen

Department of Clinical Medicine

25 Citations (Scopus)

Abstract

BACKGROUND: Tissue inhibitor of metalloproteinases-1 (TIMP-1) measurements in plasma may be useful for the early detection and prognosis of colorectal cancer (CRC). Data on analytical performance and normal intra- and interindividual biological variation are required in order to interpret the utility of TIMP-1 in CRC. The aim of this study was to establish the biological and analytical variation of plasma TIMP-1 in volunteers. MATERIAL AND METHODS: Three separate studies were undertaken. 1: Plasma was collected from 23 volunteers 6 times within a 3-week period, first in September 2004 (round [R] 1), then repeated in May 2005 (R2) and May 2006 (R3) in the same group of individuals. TIMP-1 levels were determined by the MAC15 ELISA assay and with the Abbott ARCHITECT i2000 Immunoanalyzer. 2: Circadian variation was evaluated in plasma collected 7 times within a 24-hour period (n=16). 3: Effects of physical exercise were evaluated in plasma collected before and after bicycling (n=14). In studies 2 and 3 TIMP-1 levels were determined with the MAC15 ELISA assay only. RESULTS: A significant correlation between TIMP-1 MAC15 and ARCHITECT i2000 was shown (rs=0.78, p<0.002), with consistently higher levels being detected by the ARCHITECT i2000. Median levels of TIMP-1 (ARCHITECT) at 8 a.m. in each round were 74.9 ng/mL (range 65.7-89.9) (R1), 87.3 ng/mL (range 72.7-127.9) (R2), and 81.9 ng/mL (range 66.8-113.6) (R3). The within-subject variation was 10.7%, the variation between rounds was 7.4%, and the intraclass correlation was 46.2%. Comparison between the 3 rounds and time of collection showed that TIMP-1 values decreased by 11% after storage for more than 16 months (p=0.0002). A systematic circadian variation in plasma TIMP-1 levels was not observed (p=0.17). No significant variation of plasma TIMP-1 was found in relation to physical exercise (p=0.92 [global test]). CONCLUSION: Levels of plasma TIMP-1 in volunteers show limited circadian, day-to-day, week-to-week and season-to-season variation. In addition, physical exercise has no impact on plasma TIMP-1 levels. Possible storage-dependent decreases in plasma TIMP-1 levels warrant further investigation.

Original language	English
Journal	International Journal of Biological Markers
Volume	23
Issue number	1
Pages (from-to)	42-7
Number of pages	5
ISSN	0393-6155
Publication status	Published - 2008

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Frederiksen, C. B., Lomholt, A. F., Lottenburger, T., Davis, G. J., Dowell, B. L., Blankenstein, M. A., Christensen, I. J., Brunner, N., Nielsen, H. J., Frederiksen, C., Lomholt, A. F., Lottenburger, T., Davis, G. J., Dowell, B. L., Blankenstein, M. A., Christensen, I. J., Brunner, N., & Nielsen, H. J. (2008). Assessment of the biological variation of plasma tissue inhibitor of metalloproteinases-1. International Journal of Biological Markers, 23(1), 42-7.

Frederiksen, CB, Lomholt, AF, Lottenburger, T, Davis, GJ, Dowell, BL, Blankenstein, MA, Christensen, IJ, Brunner, N, Nielsen, HJ, Frederiksen, C, Lomholt, AF, Lottenburger, T, Davis, GJ, Dowell, BL, Blankenstein, MA, Christensen, IJ, Brunner, N & Nielsen, HJ 2008, 'Assessment of the biological variation of plasma tissue inhibitor of metalloproteinases-1', International Journal of Biological Markers, vol. 23, no. 1, pp. 42-7.

@article{22331710f83d11ddb219000ea68e967b,

title = "Assessment of the biological variation of plasma tissue inhibitor of metalloproteinases-1",

abstract = "BACKGROUND: Tissue inhibitor of metalloproteinases-1 (TIMP-1) measurements in plasma may be useful for the early detection and prognosis of colorectal cancer (CRC). Data on analytical performance and normal intra- and interindividual biological variation are required in order to interpret the utility of TIMP-1 in CRC. The aim of this study was to establish the biological and analytical variation of plasma TIMP-1 in volunteers. MATERIAL AND METHODS: Three separate studies were undertaken. 1: Plasma was collected from 23 volunteers 6 times within a 3-week period, first in September 2004 (round [R] 1), then repeated in May 2005 (R2) and May 2006 (R3) in the same group of individuals. TIMP-1 levels were determined by the MAC15 ELISA assay and with the Abbott ARCHITECT i2000 Immunoanalyzer. 2: Circadian variation was evaluated in plasma collected 7 times within a 24-hour period (n=16). 3: Effects of physical exercise were evaluated in plasma collected before and after bicycling (n=14). In studies 2 and 3 TIMP-1 levels were determined with the MAC15 ELISA assay only. RESULTS: A significant correlation between TIMP-1 MAC15 and ARCHITECT i2000 was shown (rs=0.78, p<0.002), with consistently higher levels being detected by the ARCHITECT i2000. Median levels of TIMP-1 (ARCHITECT) at 8 a.m. in each round were 74.9 ng/mL (range 65.7-89.9) (R1), 87.3 ng/mL (range 72.7-127.9) (R2), and 81.9 ng/mL (range 66.8-113.6) (R3). The within-subject variation was 10.7%, the variation between rounds was 7.4%, and the intraclass correlation was 46.2%. Comparison between the 3 rounds and time of collection showed that TIMP-1 values decreased by 11% after storage for more than 16 months (p=0.0002). A systematic circadian variation in plasma TIMP-1 levels was not observed (p=0.17). No significant variation of plasma TIMP-1 was found in relation to physical exercise (p=0.92 [global test]). CONCLUSION: Levels of plasma TIMP-1 in volunteers show limited circadian, day-to-day, week-to-week and season-to-season variation. In addition, physical exercise has no impact on plasma TIMP-1 levels. Possible storage-dependent decreases in plasma TIMP-1 levels warrant further investigation.",

author = "C.B. Frederiksen and Lomholt, {Anne Fog} and Tine Lottenburger and G.J. Davis and B.L. Dowell and M.A. Blankenstein and Christensen, {Ib Jarle} and Nils Brunner and H.J. Nielsen and Camilla Frederiksen and Lomholt, {A F} and T Lottenburger and Davis, {G J} and Dowell, {B L} and Blankenstein, {M A} and Christensen, {Ib Jarle} and N Brunner and Nielsen, {Hans J{\o}rgen}",

note = "Keywords: Adult; Aged; Analysis of Variance; Blood Chemical Analysis; Circadian Rhythm; Colorectal Neoplasms; Enzyme-Linked Immunosorbent Assay; Exercise; Female; Humans; Immunoassay; Male; Middle Aged; Reference Values; Time Factors; Tissue Inhibitor of Metalloproteinase-1; Tumor Markers, Biological",

year = "2008",

language = "English",

volume = "23",

pages = "42--7",

journal = "International Journal of Biological Markers",

issn = "0393-6155",

publisher = "Wichtig Publishing",

number = "1",

}

TY - JOUR

T1 - Assessment of the biological variation of plasma tissue inhibitor of metalloproteinases-1

AU - Frederiksen, C.B.

AU - Lomholt, Anne Fog

AU - Lottenburger, Tine

AU - Davis, G.J.

AU - Dowell, B.L.

AU - Blankenstein, M.A.

AU - Christensen, Ib Jarle

AU - Brunner, Nils

AU - Nielsen, H.J.

AU - Frederiksen, Camilla

AU - Lomholt, A F

AU - Lottenburger, T

AU - Davis, G J

AU - Dowell, B L

AU - Blankenstein, M A

AU - Christensen, Ib Jarle

AU - Brunner, N

AU - Nielsen, Hans Jørgen

N1 - Keywords: Adult; Aged; Analysis of Variance; Blood Chemical Analysis; Circadian Rhythm; Colorectal Neoplasms; Enzyme-Linked Immunosorbent Assay; Exercise; Female; Humans; Immunoassay; Male; Middle Aged; Reference Values; Time Factors; Tissue Inhibitor of Metalloproteinase-1; Tumor Markers, Biological

PY - 2008

Y1 - 2008

N2 - BACKGROUND: Tissue inhibitor of metalloproteinases-1 (TIMP-1) measurements in plasma may be useful for the early detection and prognosis of colorectal cancer (CRC). Data on analytical performance and normal intra- and interindividual biological variation are required in order to interpret the utility of TIMP-1 in CRC. The aim of this study was to establish the biological and analytical variation of plasma TIMP-1 in volunteers. MATERIAL AND METHODS: Three separate studies were undertaken. 1: Plasma was collected from 23 volunteers 6 times within a 3-week period, first in September 2004 (round [R] 1), then repeated in May 2005 (R2) and May 2006 (R3) in the same group of individuals. TIMP-1 levels were determined by the MAC15 ELISA assay and with the Abbott ARCHITECT i2000 Immunoanalyzer. 2: Circadian variation was evaluated in plasma collected 7 times within a 24-hour period (n=16). 3: Effects of physical exercise were evaluated in plasma collected before and after bicycling (n=14). In studies 2 and 3 TIMP-1 levels were determined with the MAC15 ELISA assay only. RESULTS: A significant correlation between TIMP-1 MAC15 and ARCHITECT i2000 was shown (rs=0.78, p<0.002), with consistently higher levels being detected by the ARCHITECT i2000. Median levels of TIMP-1 (ARCHITECT) at 8 a.m. in each round were 74.9 ng/mL (range 65.7-89.9) (R1), 87.3 ng/mL (range 72.7-127.9) (R2), and 81.9 ng/mL (range 66.8-113.6) (R3). The within-subject variation was 10.7%, the variation between rounds was 7.4%, and the intraclass correlation was 46.2%. Comparison between the 3 rounds and time of collection showed that TIMP-1 values decreased by 11% after storage for more than 16 months (p=0.0002). A systematic circadian variation in plasma TIMP-1 levels was not observed (p=0.17). No significant variation of plasma TIMP-1 was found in relation to physical exercise (p=0.92 [global test]). CONCLUSION: Levels of plasma TIMP-1 in volunteers show limited circadian, day-to-day, week-to-week and season-to-season variation. In addition, physical exercise has no impact on plasma TIMP-1 levels. Possible storage-dependent decreases in plasma TIMP-1 levels warrant further investigation.

AB - BACKGROUND: Tissue inhibitor of metalloproteinases-1 (TIMP-1) measurements in plasma may be useful for the early detection and prognosis of colorectal cancer (CRC). Data on analytical performance and normal intra- and interindividual biological variation are required in order to interpret the utility of TIMP-1 in CRC. The aim of this study was to establish the biological and analytical variation of plasma TIMP-1 in volunteers. MATERIAL AND METHODS: Three separate studies were undertaken. 1: Plasma was collected from 23 volunteers 6 times within a 3-week period, first in September 2004 (round [R] 1), then repeated in May 2005 (R2) and May 2006 (R3) in the same group of individuals. TIMP-1 levels were determined by the MAC15 ELISA assay and with the Abbott ARCHITECT i2000 Immunoanalyzer. 2: Circadian variation was evaluated in plasma collected 7 times within a 24-hour period (n=16). 3: Effects of physical exercise were evaluated in plasma collected before and after bicycling (n=14). In studies 2 and 3 TIMP-1 levels were determined with the MAC15 ELISA assay only. RESULTS: A significant correlation between TIMP-1 MAC15 and ARCHITECT i2000 was shown (rs=0.78, p<0.002), with consistently higher levels being detected by the ARCHITECT i2000. Median levels of TIMP-1 (ARCHITECT) at 8 a.m. in each round were 74.9 ng/mL (range 65.7-89.9) (R1), 87.3 ng/mL (range 72.7-127.9) (R2), and 81.9 ng/mL (range 66.8-113.6) (R3). The within-subject variation was 10.7%, the variation between rounds was 7.4%, and the intraclass correlation was 46.2%. Comparison between the 3 rounds and time of collection showed that TIMP-1 values decreased by 11% after storage for more than 16 months (p=0.0002). A systematic circadian variation in plasma TIMP-1 levels was not observed (p=0.17). No significant variation of plasma TIMP-1 was found in relation to physical exercise (p=0.92 [global test]). CONCLUSION: Levels of plasma TIMP-1 in volunteers show limited circadian, day-to-day, week-to-week and season-to-season variation. In addition, physical exercise has no impact on plasma TIMP-1 levels. Possible storage-dependent decreases in plasma TIMP-1 levels warrant further investigation.

M3 - Journal article

SN - 0393-6155

VL - 23

SP - 42

EP - 47

JO - International Journal of Biological Markers

JF - International Journal of Biological Markers

IS - 1

ER -

Assessment of the biological variation of plasma tissue inhibitor of metalloproteinases-1

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