Assessment of satellite cell number and activity status in human skeletal muscle biopsies

TY - JOUR

T1 - Assessment of satellite cell number and activity status in human skeletal muscle biopsies

AU - Mackey, Abigail L

AU - Kjær, Michael

AU - Charifi, Nadia

AU - Henriksson, J.

AU - Bojsen-Moller, J.

AU - Holm, Lars

AU - Kadi, F.

N1 - Keywords: Adult; Antigens, CD56; Biopsy; Cell Count; Cell Proliferation; Exercise; Humans; Indoles; Ki-67 Antigen; Muscle Fibers, Skeletal; Muscle, Skeletal; Neural Cell Adhesion Molecules; PAX7 Transcription Factor; Satellite Cells, Skeletal Muscle; Time Factors; Weight Lifting; Young Adult

PY - 2009/9

Y1 - 2009/9

N2 - The primary aim of our study was to validate the assessment of myonuclear and satellite cell number in biopsies from human skeletal muscle. We found that 25 type I and 25 type II fibers are sufficient to estimate the mean number of myonuclei per fiber. In contrast, the assessment of satellite cells improved when more fibers were included. Second, we report that small differences in counting satellite cells using CD56 and Pax7 antibodies can be attributed to the different staining profiles. Third, we provide support for the use of Ki67 in evaluating the proportion of active satellite cells. We observed very few (up to 1.3%) active satellite cells in healthy adult skeletal muscle at rest, but they increased significantly (up to 7-fold) following muscle activity. This study provides valuable tools to assess the behavior of satellite cells, both in pathological conditions and in response to physiological stimuli.

AB - The primary aim of our study was to validate the assessment of myonuclear and satellite cell number in biopsies from human skeletal muscle. We found that 25 type I and 25 type II fibers are sufficient to estimate the mean number of myonuclei per fiber. In contrast, the assessment of satellite cells improved when more fibers were included. Second, we report that small differences in counting satellite cells using CD56 and Pax7 antibodies can be attributed to the different staining profiles. Third, we provide support for the use of Ki67 in evaluating the proportion of active satellite cells. We observed very few (up to 1.3%) active satellite cells in healthy adult skeletal muscle at rest, but they increased significantly (up to 7-fold) following muscle activity. This study provides valuable tools to assess the behavior of satellite cells, both in pathological conditions and in response to physiological stimuli.

U2 - 10.1002/mus.21369

DO - 10.1002/mus.21369

M3 - Journal article

C2 - 19705426

SN - 0148-639X

VL - 40

SP - 455

EP - 465

JO - Muscle & Nerve

JF - Muscle & Nerve

IS - 3

ER -