Assessment of peptidylarginine deiminase activity by ELISA using human fibrinogen as substrate

Dres Damgaard*, Claus H. Nielsen

*Corresponding author for this work
1 Citation (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) for measurement of the activity of peptidylarginine deiminases (PADs), the enzymes responsible for citrullination, is described. It uses fibrinogen as substrate for the enzyme and a commercial antibody specific for the citrullinated form of fibrinogen.

Original languageEnglish
Title of host publicationAutoantibodies : Methods and Protocols
EditorsGunnar Houen
Number of pages4
PublisherHumana Press
Publication date2019
Pages239-242
Chapter20
ISBN (Print)978-1-4939-8948-5
ISBN (Electronic)978-1-4939-8949-2
DOIs
Publication statusPublished - 2019
SeriesMethods in Molecular Biology
Volume1901
ISSN1064-3745

Keywords

  • Citrullination
  • ELISA
  • Fibrinogen
  • Peptidylarginine deiminase
  • Rheumatoid arthritis

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