Arginine vasopressin stimulates phosphoinositide turnover in an enriched rat Leydig cell preparation

J.R. Nielsen; Harald S. Hansen; B. Jensen

Arginine vasopressin stimulates phosphoinositide turnover in an enriched rat Leydig cell preparation

J.R. Nielsen, Harald S. Hansen, B. Jensen

7 Citations (Scopus)

Abstract

An enriched rat Leydig cell preparation was preincubated with [C]arachidonic acid. Stimulation of the cells with arginine vasopressin (AVP) (1 µM) for 2 min caused a significant increase in labelled phosphatidic acid and a significant fall in radioactivity in phosphatidylinositol and phosphatidylinositol 4-monophosphate + phosphatidylinositol 4,5-bisphosphate. Preincubation with dibutyryl cyclic AMP had no effect on the AVP-induced phospholipid turnover. Leydig cells were preincubated with myo-[2-H]inositol for 22 h and then with 10 mM LiCl for 10 min. Exposure to AVP (1 µM) induced a rise in labelled inositol phosphates. The response was inhibited when the cells were preincubated with the phorbol ester, 4ß-phorbol 12-myristate 13-acetate (0.16 µM) for 10 min. These results provide evidence for an AVP-induced phospholipase C stimulation in rat Leydig cells and suggest a protein kinase C-dependent feedback inhibition of the stimulation. Other agonists that might have a regulatory function in the testis were tested for possible effects on phosphoinositide metabolism. Of prostaglandin E (10 µm), angiotensin II (0.1 µM), and bradykinin (0.9 µM), only the latter induced a significant increase in the labelled inositol phosphates. This suggests that Leydig cells possess a bradykinin receptor which can activate phospholipase C.

Original language	English
Journal	Molecular and Cellular Endocrinology
Volume	61
Issue number	2
Pages (from-to)	181-188
Number of pages	8
ISSN	0303-7207
Publication status	Published - 1 Jan 1989

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title = "Arginine vasopressin stimulates phosphoinositide turnover in an enriched rat Leydig cell preparation",

abstract = "An enriched rat Leydig cell preparation was preincubated with [C]arachidonic acid. Stimulation of the cells with arginine vasopressin (AVP) (1 µM) for 2 min caused a significant increase in labelled phosphatidic acid and a significant fall in radioactivity in phosphatidylinositol and phosphatidylinositol 4-monophosphate + phosphatidylinositol 4,5-bisphosphate. Preincubation with dibutyryl cyclic AMP had no effect on the AVP-induced phospholipid turnover. Leydig cells were preincubated with myo-[2-H]inositol for 22 h and then with 10 mM LiCl for 10 min. Exposure to AVP (1 µM) induced a rise in labelled inositol phosphates. The response was inhibited when the cells were preincubated with the phorbol ester, 4{\ss}-phorbol 12-myristate 13-acetate (0.16 µM) for 10 min. These results provide evidence for an AVP-induced phospholipase C stimulation in rat Leydig cells and suggest a protein kinase C-dependent feedback inhibition of the stimulation. Other agonists that might have a regulatory function in the testis were tested for possible effects on phosphoinositide metabolism. Of prostaglandin E (10 µm), angiotensin II (0.1 µM), and bradykinin (0.9 µM), only the latter induced a significant increase in the labelled inositol phosphates. This suggests that Leydig cells possess a bradykinin receptor which can activate phospholipase C.",

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AU - Nielsen, J.R.

AU - Hansen, Harald S.

AU - Jensen, B.

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N2 - An enriched rat Leydig cell preparation was preincubated with [C]arachidonic acid. Stimulation of the cells with arginine vasopressin (AVP) (1 µM) for 2 min caused a significant increase in labelled phosphatidic acid and a significant fall in radioactivity in phosphatidylinositol and phosphatidylinositol 4-monophosphate + phosphatidylinositol 4,5-bisphosphate. Preincubation with dibutyryl cyclic AMP had no effect on the AVP-induced phospholipid turnover. Leydig cells were preincubated with myo-[2-H]inositol for 22 h and then with 10 mM LiCl for 10 min. Exposure to AVP (1 µM) induced a rise in labelled inositol phosphates. The response was inhibited when the cells were preincubated with the phorbol ester, 4ß-phorbol 12-myristate 13-acetate (0.16 µM) for 10 min. These results provide evidence for an AVP-induced phospholipase C stimulation in rat Leydig cells and suggest a protein kinase C-dependent feedback inhibition of the stimulation. Other agonists that might have a regulatory function in the testis were tested for possible effects on phosphoinositide metabolism. Of prostaglandin E (10 µm), angiotensin II (0.1 µM), and bradykinin (0.9 µM), only the latter induced a significant increase in the labelled inositol phosphates. This suggests that Leydig cells possess a bradykinin receptor which can activate phospholipase C.

AB - An enriched rat Leydig cell preparation was preincubated with [C]arachidonic acid. Stimulation of the cells with arginine vasopressin (AVP) (1 µM) for 2 min caused a significant increase in labelled phosphatidic acid and a significant fall in radioactivity in phosphatidylinositol and phosphatidylinositol 4-monophosphate + phosphatidylinositol 4,5-bisphosphate. Preincubation with dibutyryl cyclic AMP had no effect on the AVP-induced phospholipid turnover. Leydig cells were preincubated with myo-[2-H]inositol for 22 h and then with 10 mM LiCl for 10 min. Exposure to AVP (1 µM) induced a rise in labelled inositol phosphates. The response was inhibited when the cells were preincubated with the phorbol ester, 4ß-phorbol 12-myristate 13-acetate (0.16 µM) for 10 min. These results provide evidence for an AVP-induced phospholipase C stimulation in rat Leydig cells and suggest a protein kinase C-dependent feedback inhibition of the stimulation. Other agonists that might have a regulatory function in the testis were tested for possible effects on phosphoinositide metabolism. Of prostaglandin E (10 µm), angiotensin II (0.1 µM), and bradykinin (0.9 µM), only the latter induced a significant increase in the labelled inositol phosphates. This suggests that Leydig cells possess a bradykinin receptor which can activate phospholipase C.

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AN - SCOPUS:0024501126

SN - 0303-7207

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JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

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ER -

Arginine vasopressin stimulates phosphoinositide turnover in an enriched rat Leydig cell preparation

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