Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates

C J Grimmelikhuijzen; Ditte Graff Ebbesen

Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates

C J Grimmelikhuijzen, Ditte Graff Ebbesen

Cell and Neurobiology

38 Citations (Scopus)

Abstract

By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.

Original language	English
Journal	Peptides
Volume	6 Suppl 3
Pages (from-to)	477-83
Number of pages	7
ISSN	0196-9781
Publication status	Published - 1 Jan 1985

Keywords

Animals
Chromatography
Cnidaria
FMRFamide
Fluorescent Antibody Technique
Nerve Tissue Proteins
Nervous System Physiological Phenomena
Oligopeptides
Radioimmunoassay

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title = "Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates",

abstract = "By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.",

keywords = "Animals, Chromatography, Cnidaria, FMRFamide, Fluorescent Antibody Technique, Nerve Tissue Proteins, Nervous System Physiological Phenomena, Oligopeptides, Radioimmunoassay",

author = "Grimmelikhuijzen, {C J} and Ebbesen, {Ditte Graff}",

year = "1985",

month = jan,

day = "1",

language = "English",

volume = "6 Suppl 3",

pages = "477--83",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier",

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TY - JOUR

T1 - Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates

AU - Grimmelikhuijzen, C J

AU - Ebbesen, Ditte Graff

PY - 1985/1/1

Y1 - 1985/1/1

N2 - By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.

AB - By using immunocytochemistry and radioimmunoassays, several substances resembling vertebrate or invertebrate neuropeptides have been found in the nervous systems of coelenterates. The most abundant neuropeptides were those related to the molluscan neuropeptide Phe-Met-Arg-Phe-amide (FMRFamide). Of antisera against different fragments of FMRFamide, those against RFamide were superior in recognizing the coelenterate peptide. Incubation of whole mounts with these RFamide antisera visualized the coelenterate nervous system in such a detail as has previously not been possible. By using a radioimmunoassay with a RFamide antiserum and [J-125]-YFMRFamide as tracer, the RFamide-like peptide from sea anemones was isolated. After cation-exchange chromatography, gelfiltration and HPLC, this peptide was obtained in a pure form.

KW - Animals

KW - Chromatography

KW - Cnidaria

KW - FMRFamide

KW - Fluorescent Antibody Technique

KW - Nerve Tissue Proteins

KW - Nervous System Physiological Phenomena

KW - Oligopeptides

KW - Radioimmunoassay

M3 - Journal article

C2 - 2870476

SN - 0196-9781

VL - 6 Suppl 3

SP - 477

EP - 483

JO - Peptides

JF - Peptides

ER -

Arg-Phe-amide-like peptides in the primitive nervous systems of coelenterates

Abstract

Keywords

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