Arabinoxylan oligosaccharides and polyunsaturated fatty acid effects on gut microbiota and metabolic markers in overweight individuals with signs of metabolic syndrome: A randomized cross-over trial

TY - JOUR

T1 - Arabinoxylan oligosaccharides and polyunsaturated fatty acid effects on gut microbiota and metabolic markers in overweight individuals with signs of metabolic syndrome

T2 - A randomized cross-over trial

AU - Kjølbæk, Louise

AU - Benítez-Páez, Alfonso

AU - Gómez Del Pulgar, Eva M

AU - Brahe, Lena Kirchner

AU - Liebisch, Gerhard

AU - Matysik, Silke

AU - Rampelli, Simone

AU - Vermeiren, Joan

AU - Brigidi, Patrizia

AU - Larsen, Lesli Hingstrup

AU - Astrup, Arne

AU - Sanz, Yolanda

N1 - CURIS 2019 NEXS 083 Copyright © 2019 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

PY - 2020/1

Y1 - 2020/1

N2 - Background & aims: Gut microbiota composition is linked to obesity and metabolic syndrome. The nutrients and doses required to modulate the gut microbiota towards beneficially influence components of the metabolic syndrome are unclear. This study aimed to investigate diet-induced effects on the gut microbiota and metabolic markers in overweight individuals with indices of the metabolic syndrome. Methods: A twelve-week randomized cross-over trial was conducted with two intervention periods separated by a washout period. The dietary intakes of interest were wheat bran extract, rich in arabinoxylan oligosaccharides (AXOS) (10.4 g/d AXOS) and polyunsaturated fatty acids (PUFA) (3.6 g/d n-3 PUFA). Dietary records, fecal and blood samples, as well as anthropometric data, were collected before and after intervention. Anthropometry and gastrointestinal symptoms were evaluated weekly. Gut microbiota composition was analyzed by massive sequencing of 16S ribosomal RNA gene V3[sbnd]V4 amplicons. Results: Twenty-seven participants completed the study (90%). Intake of AXOS induced an expected bifidogenic effect on gut microbiota (p < 0.01) and increased butyrate-producing bacterial species as well (p < 0.05). Beta-diversity analysis indicated that the structure of the gut microbiota only changed as a result of the AXOS intervention (Permanova = 1.90, p < 0.02) and no changes in metabolic markers were observed after any of the interventions. Conclusions: AXOS intake has a bifidogenic effect and also increases butyrate producers in the gut microbiota; even though this type of dietary fiber did not modulate lipid or glucose metabolic parameters related to metabolic syndrome. Four-week PUFA intake did not induce any notable effect on the gut microbiota composition or metabolic risk markers. Registration: Registered under ClinicalTrials.gov Identifier no. NCT02215343. Clinical trial registration: Registered at https://www.clinicaltrials.gov/ (NCT02215343). Ethical committee: H-4-2014-052. The Danish Data Protection Agency: 2013-54-0522.

AB - Background & aims: Gut microbiota composition is linked to obesity and metabolic syndrome. The nutrients and doses required to modulate the gut microbiota towards beneficially influence components of the metabolic syndrome are unclear. This study aimed to investigate diet-induced effects on the gut microbiota and metabolic markers in overweight individuals with indices of the metabolic syndrome. Methods: A twelve-week randomized cross-over trial was conducted with two intervention periods separated by a washout period. The dietary intakes of interest were wheat bran extract, rich in arabinoxylan oligosaccharides (AXOS) (10.4 g/d AXOS) and polyunsaturated fatty acids (PUFA) (3.6 g/d n-3 PUFA). Dietary records, fecal and blood samples, as well as anthropometric data, were collected before and after intervention. Anthropometry and gastrointestinal symptoms were evaluated weekly. Gut microbiota composition was analyzed by massive sequencing of 16S ribosomal RNA gene V3[sbnd]V4 amplicons. Results: Twenty-seven participants completed the study (90%). Intake of AXOS induced an expected bifidogenic effect on gut microbiota (p < 0.01) and increased butyrate-producing bacterial species as well (p < 0.05). Beta-diversity analysis indicated that the structure of the gut microbiota only changed as a result of the AXOS intervention (Permanova = 1.90, p < 0.02) and no changes in metabolic markers were observed after any of the interventions. Conclusions: AXOS intake has a bifidogenic effect and also increases butyrate producers in the gut microbiota; even though this type of dietary fiber did not modulate lipid or glucose metabolic parameters related to metabolic syndrome. Four-week PUFA intake did not induce any notable effect on the gut microbiota composition or metabolic risk markers. Registration: Registered under ClinicalTrials.gov Identifier no. NCT02215343. Clinical trial registration: Registered at https://www.clinicaltrials.gov/ (NCT02215343). Ethical committee: H-4-2014-052. The Danish Data Protection Agency: 2013-54-0522.

KW - Faculty of Science

KW - Gut microbiota

KW - Arabinoxylan oligosaccharide

KW - Fiber

KW - Fish oil

KW - Metabolic syndrome

KW - Obesity

U2 - 10.1016/j.clnu.2019.01.012

DO - 10.1016/j.clnu.2019.01.012

M3 - Journal article

C2 - 30827722

SN - 0261-5614

JO - Clinical Nutrition

JF - Clinical Nutrition

ER -